QF0159 Marketing Release Record

Corynebacterium diphtheriae
100 Tests primer set NCL-CD-PS O
Large outbreaks of diphtheria in eastern Europe and modern travel have led
to an increased awareness of Corynebacterium diphtheriae. Most western
isolates are still non-toxigenic, but toxin tests require experience and
expertise.
Product Specific Information
The Corynebacterium diphtheriae primer set, NCL-CD-PS, consists of a pair
of oligonucleotide primers (CD-1 and CD-2) which amplify a 246bp region of
the toxin gene alpha subunit. Also provided is an internal control DNA for
aiding in the detection of false-negative PCR results. The internal control DNA
may be added to the test PCR, where it will amplify with the same primers but
yield a product of different size.
Agarose gel: electrophoresis of PCR products using NCL-CD-PS. Lane A, 100bp ladder
(Promega). Lane B, Reagent blank. Lanes C-F and H, five toxigenic Corynebacterium
diphtheriae isolates. Lane G, non-toxigenic Corynebacterium diphtheriae isolate. The
polymerase chain reaction (PCR) process is covered by patents owned by Hoffman Laroche
Inc. and Hoffman La-Roche Ltd. Purchase of these products does not carry a licence to
perform the PCR process.
Mycobacterium tuberculosis
100 Tests primer set NCL-MT-PS O
RUO* RUO*
There is a rising incidence of tuberculosis, an emergence of drug-resistant
strains of Mycobacterium tuberculosis and an overlap with AIDS. Direct
detection and identification of Mycobacterium tuberculosis in sputum is
possible by PCR. Some low level infections may be missed (but later
detected by culture), but it is reported that PCR will certainly identify all
strains of Mycobacterium tuberculosis detected by conventional
microscopy (60 to 80 percent).
Product Specific Information
The Mycobacterium tuberculosis primer set, NCL-MT-PS, consists of a pair
of oligonucleotide primers (MT-1 and MT-2) which amplify the 123bp insertion
sequence IS6110. The sequence is unique to members of the Mycobacterium
tuberculosis complex (MTBC) and is present as a sequence in multiple copies
in many common isolates. Also provided is an internal control DNA for aiding
in the detection of false-negative PCR results.
Agarose gel: electrophoresis of PCR products using NCL-MT-PS. Lane A, 123bp ladder (Sigma).
Lane B, internal control only. Lanes C, D and E, Mycobacterium tuberculosis, Mycobacterium
avium and Mycobacterium intracellulare isolates, respectively. Lanes F and G, Mycobacterium
tuberculosis-positive human sputa. Lane H, Mycobacterium tuberculosis-positive, formalinfixed,
paraffin-embedded human lung. The polymerase chain reaction (PCR) process is
covered by patents owned by Hoffman Laroche Inc. and Hoffman La-Roche Ltd. Purchase
of these products does not carry a licence to perform the PCR process.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primer Sets