QF0159 Marketing Release Record

Primary Antibodies
Novocastra CD23
Clone 1B12 Reference Range
1 mL, 0.1 mL lyophilized NCL-CD23-1B12 F P (HIER)
1 mL liquid NCL-L-CD23-1B12 F P (HIER) C IVD
7 mL ready-to-use RTU-CD23-1B12 F P (HIER) IVD
7 mL BOND ready-to-use PA0169 P (HIER) IVD
Antigen Background
The CD23 molecule is the low affinity IgE receptor found on B cells. It is a
membrane glycoprotein of 45 kD and is reported to be found on a subpopulation
of peripheral blood cells, B lymphocytes and on EBV-transformed
B lymphoblastoid cell lines. Expression of CD23 antigen has been reported on
monocytes and dendritic cells.
Novocastra CD25 (Interleukin-2
Receptor)
Clone 4C9
1 mL lyophilized NCL-CD25-305 ASR
7 mL BOND ready-to-use PA0305 P (HIER)
See also Interleukin-2 Receptor (CD25) on page 76.
Novocastra CD29
Clone 7F10
1 mL, 0.1 mL lyophilized NCL-CD29 P (HIER)
The �1 integrins are a family of structurally-related heterodimeric molecules
and are composed of a �1 subunit (CD29 antigen) which is associated with 1
of 6 known alpha subunits. These impart the specificity to each of the
receptors and the VLA molecules which are designated according to their
alpha chain eg VLA-1 is �1/�1, VLA-2 is �2/�1. The adhesive properties of
CD29 heterodimers on T cells can be regulated by cell activation, possibly
through interactions between the cytoplasmic domain of CD29 antigen and
the cytoskeleton. CD29 antigen is reported to be expressed on most cells
including all leukocytes, although only at low levels on granulocytes. On
T cells, CD29 antigen is expressed at higher levels on memory cells than on
naive cells. The co-expression of CD4 and CD29 antigens is found in helper/
inducer subpopulation of CD4 lymphocytes. CD29 antigen is one of several
additional molecules reported to be found on the cell membrane of
hepatocytes in cases of cirrhosis, alcoholic hepatitis and hepatitis C.
Reduced expression of CD29 antigen together with the �2 integrin, CD11b,
has been reported on peripheral blood lymphocytes from Graves' disease
patients.
Human brain, cerebellum: immunohistochemical staining for CD29 antigen using NCL-CD29.
Note membrane staining of endothelial cells. Paraffin section.
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RUO*
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Novocastra CD30
Clone JCM182 Reference Range
1 mL, 0.1 mL liquid NCL-L-CD30-591 P (HIER) W
7 mL BOND ready-to-use PA0790 P (HIER) IVD
Clone 1G12
1 mL, 0.1mL lyophilized NCL-CD30 F P (HIER)
1 mL liquid NCL-L-CD30 F P (HIER)
7 mL ready-to-use RTU-CD30 F P (HIER)
7 mL BOND ready-to-use PA0153 P (HIER)
Clone 15B3
1 mL, 0.1 mL lyophilized NCL-CD30-365 F P (HIER) RUO*
Clone JCM182 was developed to be highly effective on formalin-fixed,
paraffin-embedded tissue sections.
Antigen Background
The CD30 antigen is a single chain glycoprotein with a molecular weight of
120 kD. CD30 antigen is known to act as a receptor for a cytokine ligand,
CD30L, and may also play a role in the regulation of cellular growth and
transformation. CD30 antigen is reported to be expressed on the surface of
multinucleated Reed Sternberg cells, mononuclear Hodgkin's cells and in the
majority of anaplastic large cell lymphomas. The CD30 antigen is expressed
in non-Hodgkin's lymphoma and virally transformed cells, eg EBVtransformed
B cells. Most T cell lymphomas are reported not to express CD30
antigen, with the exception of some pleo-morphic T cell lymphomas.
Product Specific Information
Using retrieval solutions other than that recommended for Clone JCM182 in
the datasheet may increase background reactivity.
Hodgkin's lymphoma: immunohistochemical staining for CD30 antigen using NCL-L-CD30-591.
Note membrane staining and characteristic staining of paranuclear hofs of Reed Sternberg
cells. Paraffin section.
Novocastra CD31 (PECAM-1)
Clone 1A10
1 mL, 0.1 mL lyophilized NCL-CD31-1A10 P (HIER)
7 mL BOND ready-to-use PA0250 P (HIER) IVD
Antigen Background
CD31 antigen (PECAM-1) is a single chain transmembrane glycoprotein with a
molecular weight of 130 to 140 kD. The CD31 molecule is expressed on the
surface of platelets, monocytes, granulocytes, B cells and at the endothelial
intracellular junction. The molecule has an extracellular domain that contains
six Ig-like homology units of C2 subclass, typical of cell to cell adhesion
molecules. This domain mediates endothelial cell to cell adhesion, plays a role
in endothelial contact and may serve to stabilize the endothelial cell monolayer.
The CD31 molecule also has a cytoplasmic domain with potential sites for
phosphorylation after cellular activation. The properties of CD31 antigen
suggest that it is involved in interactive events during angiogenesis, thrombosis
and wound healing. Angiogenesis is essential for tumor growth and metastases.
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Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
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