QF0159 Marketing Release Record

Primary Antibodies
Novocastra Multi-Cytokeratin
Clone AE1, Clone AE3
1 mL lyophilized NCL-AE1/AE3 F P (HIER)
1 mL liquid NCL-L-AE1/AE3 F P (HIER)
7 mL ready-to-use RTU-AE1/AE3 F P (HIER)
7 mL BOND ready-to-use PA0909 P (Enzyme)
Antigen Background
Keratins are a family of water insoluble proteins of 40 to 70 kD. These proteins
form tonofilaments, a class of intermediate filament, in epidermis as well as
in almost all other epithelia. The process of normal epidermal differentiation
is characterized by a series of morphological and bio-chemical changes as
cells progress from the germinative basal layer through the spinous and
granular layers to the outer cornified layer. The 65 to 67 kD cytokeratins are
reported to be present only above the basal layer, the 58 kD cytokeratin is
reported to be expressed throughout the entire epidermis including the basal
layer and the 56 kD cytokeratin is reported to be absent from the basal layer
and is normally eliminated during stratum corneum formation. The 56 and 65
to 67 kD cytokeratins are reported to be characteristic of epidermal cells
undergoing terminal differentiation and may be considered as molecular
markers for keratinization.
Product Specific Information
Clones AE1 and AE3 are specific for the 56.5, 50, 50', 48 and 40 kD acidic
cytokeratins as well as the 65 to 67, 64, 59, 58, 56 and 52 kD basic cytokeratins.
The cocktail of clones AE1 and AE3 exhibit broad reactivity with two families
of cytokeratin, acidic and basic.
Human benign nodular hyperplasia of prostate: immunohistochemical staining for cytokeratins
using RTU-AE1/AE3. Note intense cytoplasmic staining of glandular myoepithelial cells.
Paraffin section.
Novocastra Multi-Cytokeratin
(4/5/6/8/10/13/18)
Clone C-11
1 mL lyophilized NCL-C11 F P (HIER)
Product Specific Information
Cytokeratins 4, 5, 6, 8, 10, 13 and 18 are differentially expressed between a
variety of normal, reactive and neoplastic epithelia and also simple
epithelium and both basal and suprabasal layers of cornifying and noncornifying
squamous epithelium.
NCL-C11 is reported to react with human cytokeratins 4, 5, 6, 8, 10, 13 and 18.
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IVD
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Novocastra Multi-Cytokeratin 1/5/10/14
Clone 34�E12
1 mL lyophilized NCL-CK34BE12 F P (HIER) W
7 mL ready-to-use RTU-CK34BE12 F P (HIER)
7 mL BOND ready-to-use PA0134 P (Enzyme)
Antigen Background
NCL-CK34�E12 reacts with human cytokeratin intermediate filament proteins
1, 5, 10 and 14. The antibody is reported to react with squamous epithelium
and sweat ducts in normal skin, some pneumocytes, bronchial epithelium and
mesothelium in normal lung and bile ducts in normal liver. It also reacts with
ductal cells of the normal pancreas, some acinar and ductal cells of normal
breast, some follicular epithelia of normal thyroid and some epithelia and
mesothelium of the normal small and large bowel.
Novocastra Multi-Cytokeratin (5/6/8/18)
5D3, Clone LP34
1 mL lyophilized NCL-CK5/6/8/18 F P (Enzyme)
1 mL liquid NCL-L-CK5/6/8/18 F P (Enzyme)
7 mL ready-to-use RTU-CK5/6/8/18 F P (Enzyme)
Product Specific Information
NCL-CK5/6/8/18, NCL-L-CK5/6/8/18 and RTU-CK5/6/8/18 react with human
cytokeratins 5, 6, 8 and 18. These products are cocktails of monoclonal
antibodies designed to recognize cytokeratins reported to be expressed in
almost all epithelial tissues.
Human squamous cell carcinoma of the floor of the mouth: immunohistochemical staining for
cytokeratins using NCL-L-CK5/6/8/18. Note intense cytoplasmic staining of malignant cells.
Paraffin section.
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
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