QF0159 Marketing Release Record

Novocastra p21 (WAF1 Protein)
Clone 4D10
1 mL, 0.1 mL lyophilized NCL-WAF-1 P (HIER)
1 mL liquid NCL-L-WAF-1 P (HIER) RUO*
See also WAF1 Protein (p21, C1P1) on page 111.
Novocastra p27 Protein
Clone 1B4
1 mL, 0.1 mL lyophilized NCL-p27 P (HIER) W
p27 protein, also known as kinase inhibitory protein 1 (Kip1), binds to cyclin
E/cdk2 complexes (but not to cdk2 alone) and is detected in purified extracts
of growth-arrested cells. p27 protein constrains cell proliferation by setting
the threshold level of cyclin E necessary to activate cdk2. The 27 kD protein
is also present in proliferating cells but only in a sequestered form when it is
unavailable to interact with cyclin E/cdk2 complexes. It is likely that cyclin D
complexed with catalytically inactive cdk4 is sufficient to sequester p27
protein and titrate its function. The presence of bound p27 protein in
proliferating cells suggests that its role may not be restricted to inducing cell
cycle arrest but to also set the cyclin E threshold for execution of the G1 to S
phase transition during each mitotic cycle.
Novocastra p53 Protein
RUO*
RUO*
Clone IMX25
1 mL, 0.1 mL lyophilized NCL-p53-505 P (HIER) W
7 mL BOND ready-to-use PA0057 P (HIER) IVD
RUO*
p53 protein plays a vital role in suppressing the development of cancer. The
accumulation of p53 protein in response to DNA damage in vitro is well
established and appears to induce growth arrest and apoptosis by the
transcriptional regulation of other genes. In irradiated mice, p53 protein
accumulates in splenocytes, thymocytes and osteocytes, though not in
hepatocytes. Mouse T3T3 cells express high levels of phenotypically
characteristic wild type p53 protein which carries two missense mutations.
The range of antigenic sites in mouse p53 protein and human p53 protein is
very similar.
Product Specific Information
NCL-p53-505 is raised to the same recombinant mouse p53 protein as the
polyclonal, NCL-p53-CM5p. It reacts with rat and mouse p53 protein.
Mouse T3T3 cells: immunohistochemical staining for p53 mouse protein using NCL-p53-505.
Note intense nuclear staining of a proportion of T3T3 cells. Paraffin section.
Novocastra p53 Protein (BP53-12)
Clone BP53-12
1 mL lyophilized NCL-p53-BP FPW
p53 protein plays a vital role in suppressing the development of cancer. The
accumulation of p53 protein in response to DNA damage in vitro is well
established and appears to induce growth arrest and apoptosis by the
transcriptional regulation of other genes.
Product Specific Information
Clone BP53-12 recognizes both wild type and mutant forms of human p53
protein under denaturing and non-denaturing conditions. The heat induced
epitope retrieval technique may improve staining in some cases.
Human breast carcinoma: immunohistochemical staining for p53 protein using NCL-p53-BP.
Note intense nuclear staining of tumor cells. Paraffin section.
Novocastra p53 Protein (CM1)
Polyclonal
0.2 mL lyophilized NCL-p53-CM1 F P (HIER) W
Antigen Background
RUO*
Mutation of the p53 protein may represent the commonest genetic event in
human malignancy. In colonic tumors, p53 protein has been reported to be
overexpressed in 47 percent of carcinomas and only 9 percent of adenomas.
No expression has been reported in normal mucosa.
Product Specific Information
This polyclonal antibody recognizes both wild type and mutant forms of
human p53 protein under denaturing and non-denaturing conditions. NCLp53-CM1
is less sensitive to overfixation than clone DO-7.
IVD
Axillary lymph node infiltrated by metastatic breast carcinoma: immunohistochemical staining
for p53 protein using NCL-p53-CM1. Note intense nuclear staining of a proportion of tumor
cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies