QF0159 Marketing Release Record

BOND
BOND Epitope Retrieval Solution 1
1 L AR9961 P
Components
BOND Epitope Retrieval Solution 1 is a 1 L ready-to-use, citrate based pH 6.0
solution. It is specifically for heat-induced epitope retrieval (HIER) on the
automated BOND system.
Application
BOND Epitope Retrieval Solution 1 is for use on formalin-fixed, paraffinembedded
tissue sections to expose epitopes within tissue that have been
masked during fixation. The solution is gentle on sections as it has a reduced
boiling temperature and utilizes BOND's Covertile technology to prevent
reagent evaporation. Some antibodies, such as BOND ready-to-use Thyroid
Transcription Factor-1, stain optimally when used with BOND Epitope
Retrieval Solution 1. Testing should be carried out to optimize per-formance.
Human lung stained for TTF-1 with BOND ready-to-use Thyroid Transcription Factor-1 (SPT24,
PA0364), using BOND Polymer Refine Detection and BOND Epitope Retrieval Solution 1.
BOND Epitope Retrieval Solution 2
1 L AR9640 P
Components
BOND Epitope Retrieval Solution 2 is a 1 L ready-to-use, EDTA based pH 9.0
solution. It is specifically for heat-induced epitope retrieval (HIER) on the
BOND system.
Application
BOND Epitope Retrieval Solution 2 is for use on formalin-fixed, paraffinembedded
tissue sections to expose epitopes within tissue that have been
masked during fixation. The solution is gentle on sections as it has a
reduced boiling temperature and utilizes BOND's Covertile technology to
prevent reagent evaporation. Some antibodies, such as BOND ready-to-use
CD3 (LN10), stain optimally when used with BOND Epitope Retrieval
Solution 2. Testing should be carried out to optimize performance.
Human tonsil stained for CD3 with BOND ready-to-use CD3 (LN10, PA0533), using BOND
Polymer Refine Detection and BOND Epitope Retrieval Solution 2.
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Anti-Biotin Antibody
7.5 mL ready-to-use AR0584 P
Components
Anti-Biotin Antibody is a purified anti-biotin, IgG1 isotype. It is supplied
ready-to-use.
Application
In situ hybridization (ISH) allows the detection and visualization of specific
nucleic acids in tissue sections. ISH probes used for detection of DNA on
the BOND contain a biotin label. The Anti-Biotin Antibody allows the linking
of the probe with the detection reagents and consequently visualization of a
chromogenic product by light microscopy.
Anti-Fluorescein Antibody
3.75 mL AR0833 P
15 mL AR0222 P
Components
Anti-Fluorescein Antibody is a purified IgG fraction of a mouse monoclonal
antibody. It is supplied ready-to-use.
Application
In situ hybridization (ISH) allows the detection and visualization of specific
nucleic acids in tissues sections. ISH probes used for the detection of
mRNA on BOND contain a fluorescein label. The Anti-Fluorescein Antibody
allows linking of the oligonucleotide probe with the detection reagents, and
consequently, visualization of a chromogenic product by light microscopy.
BOND Hybridization Solution
100 mL AR9013 RUO*
BOND Hybridization Solution is intended to be used for the dilution of
individual In situ hybridization (ISH) probes for use on the automated Bond
system.
Stringency Wash
6.25 mL AR0633 P
Components
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The Stringency Wash Solution is a formamide mixture used with the BOND
DNA Probes. This solution reduces non-specific hybridization of DNA
probes.
Application
In situ hybridization (ISH) allows the detection and visualization of specific
nucleic acids in tissue sections. The Stringency Wash Solution is intended
for use with biotin conjugated DNA probes to reduce non-specific DNA
hybridization in formalin-fixed, paraffin-embedded tissue using the automated
BOND system.
IVD
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.