QF0159 Marketing Release Record

Novocastra Gross Cystic Disease Fluid
Protein-15
Clone 23A3
1 mL, 0.1 mL lyophilized NCL-GCDFP15 P (HIER)
1 mL liquid NCL-L-GCDFP15 P (HIER)
7 mL ready-to-use RTU-GCDFP15 P (HIER)
7 mL BOND ready-to-use PA0350 P (HIER)
Antigen Background
Gross cystic disease of the breast is a benign premenopausal disorder in
which cysts are a predominant pathological lesion. These cysts appear to be
formed from excessive apocrine cystic secretions. This fluid is composed of
several glycoproteins including a unique 15 kD monomer protein, GCDFP15. It
has been reported that cytosolic analysis of normal tissue from all major
organs has demonstrated GCDFP15 in apocrine epithelia, lacrimal,
ceruminous and Moll's glands and in numerous serous cells of the
submandibular, tracheal, bronchial, sublingual and minor salivary glands.
Cytosol from breast carcinoma lesions are reported to contain GCDFP15 at a
wide range of concentrations. The concentration is reported to be highest in
more differentiated carcinomas and GCDFP15 shows only a few positive
individual epithelial cells within lobules and small ducts in normal breast.
Expression has also been reported in fibroadenomas within areas of apocrine
metaplasia. GCDFP15 and prostate specific antigen are reported to be coexpressed
in androgen receptor-positive breast tumors.
Human breast, Paget's disease: immunohistochemical staining for gross cystic disease fluid
protein (15 kD) using RTU-GCDFP15. Note variable cytoplasmic staining of tumor cells. Paraffin
section.
Novocastra Heat Shock Protein 27
Clone 2B4
1 mL lyophilized NCL-HSP27 FPW
Prokaryotes and eukaryotes express a variety of heat shock proteins (Hsps)
in response to stress, including sublethal heat shock, exposure to heavy
metals, hormones and viral infection. Hsp27 (27 kD) is the most common
small Hsp found in man. In breast tissue, it is reported that expression of
Hsp27 is taken as evidence of a functional estrogen receptor pathway.
Human breast carcinoma: immunohistochemical staining for Hsp27 using NCL-HSP27.
Note intense cytoplasmic staining of tumor cells. Paraffin section.
IVD
RUO*
IVD
IVD
IVD
Novocastra Heat Shock Protein 70
Clone 8B11
1 mL lyophilized NCL-HSP70 P (HIER)
The response of cells or organisms to stress, such as exposure to heat or
chemicals, is associated with the induction of heat shock proteins. Heat
shock protein 70 (Hsp70) is reported to have a protective role in ischemic
disease, inflammation, infection and a potential role in antigen processing
as well as a possible regulatory role in cytokine biosynthesis. Hsp70 exists in
the cell in equilibrium between its free state, in the cytoplasm, and its bound
state, protecting proteins in the nucleolus, perhaps either by helping refold
some of the unfolded ribosomal proteins or by solubilizing the denatured
ribosomal proteins to facilitate their turnover. During recovery from heat
shock and as the nucleoli begin to return to their normal activities, most of
the Hsp70 returns to the cytoplasm.
Product Specific Information
NCL-HSP70 is reactive with Hsp70 and heat shock cognate 70 (Hsc70) in man,
mouse and rat.
Novocastra Heat Shock Protein 105
Clone 58F12
1 mL lyophilized NCL-HSP105 P (HIER) W
Heat shock protein 105 (Hsp105) exists as two isoforms; alpha and beta
which belong to the Hsp105/Hsp110 protein family. Hsp105 acts as both a
chaperone to prevent thermal aggregation of proteins and as a regulator of
mammalian cells. The Hsp105 isoforms are reported to be found in the
cytoplasm but not in the nucleoli under non-stressed and stressed conditions.
In rodents, Hsp105 isoforms are reported to be moderately expressed
in the adrenal glands, spleen, liver and heart and both are markedly
increased after heat shock. In the testis, Hsp105 is specifically localized in
the cytoplasm of germ cells but may translocate to the nucleus after heat
shock. The most abundant expression of Hsp105 occurs in the brain with
nuclear and cytoplasmic expression in nearly all neurons, oligodendrocytes,
microglia and astrocytes. Increased expression reported during
embryogenesis suggests that Hsp105 may have an important role during
mouse development.
Human brain, cerebellum: immunohistochemical staining for heat shock protein 105 using
NCL-HSP105. Note intense cytoplasmic and nuclear staining of Purkinje cells and neuronal
processes. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
RUO*
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Primary Antibodies