QF0159 Marketing Release Record

Primary Antibodies
Human tonsil: immunohistochemical staining for plasma cells using NCL-PC. Note cytoplasmic
staining of plasma cells. Paraffin section.
Novocastra Plasminogen Activator
Inhibitor (Type 1)
Clone TJA6
1 mL, 0.1 mL lyophilized NCL-PAI-1 P
Plasminogen activator inhibitor (Type 1, PAI-1) is a 48 kD protein which
inhibits the conversion of plasminogen to plasmin. It is the principal inhibitor
of the plasminogen activators t-PA and u-PA. PAI-1 is structurally related to
the serine protease inhibitor (serpin) superfamily. The serpins are known to
undergo a conformational rearrangement upon cleavage of the reactive
central peptide bond (P 1 -P 1' ) and it is this conformational difference
between the active and cleaved forms which determine their reactivity. The
PAI-1 protein is reported to be overexpressed in a number of malignancies
including ovarian cancers and derived cell lines, endometrial cancers,
cervical cancer, malignant brain cancers, nasopharyngeal carcinomas,
breast tumors, head and neck squamous cell carcinomas and gastric
cancers. PAI-1 is also reported to be expressed by endothelial cells and is
stored in platelets.
Human ovarian adenocarcinoma: immunohistochemical staining for plasminogen activator
inhibitor type 1 using NCL-PAI-1. Note intense cytoplasmic staining of malignant tumor cells.
Paraffin section.
Novocastra Platelet-Derived
Endothelial Growth Factor
Clone P-GF.44C
1 mL lyophilized NCL-PDEGF P (HIER) W
Angiogenesis, the formation of new blood vessels from an existing vascular
bed is a complex multi-step process. It is controlled by a number of
angiogenic factors, one of which is platelet-derived endothelial growth
factor (PDEGF) also shown to be thymidine phosphorylase (TP).
Angiogenesis is tightly regulated and is observed only transiently during
reproduction, development and wound healing. PDEGF is reported to be
expressed in the nucleus and cytoplasm, the highest expression being
described in macrophages, stromal cells, glial cells and some epithelia. No
expression is reported in gastro-intestinal epithelium, smooth muscle,
adrenal glands, lung and testis. The high expression in macrophages and
skin may be important for total body thymidine homeostasis.
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RUO*
RUO*
Western blot: detection of platelet-derived endothelial growth factor (53 kD) using NCL-PDEGF.
Lane A, molecular weight markers. Lane B, human tonsil immunoblotted with NCL-PDEGF.
Novocastra Pneumolysin
Clone 9.1/2/3/6
1 mL lyophilized NCL-SPNm W
RUO*
Pneumolysin is a 53 kD species-specific protein produced by all important
strains of Streptococcus pneumoniae (pneumococcus), an organism which
causes a wide range of human disease, from sinusitis and pneumonia to
septicaemia and meningitis.
Western blot: detection of pneumolysin (53 kD) using NCL-SPNm. Lane A, molecular weight
markers. Lanes B to E, Streptococcus pneumoniae culture-positive sputum samples. Lane F,
Streptococcus pneumoniae culture-negative but pneumolysin PCR-positive nasopharyngeal
secretion. Lane G, Streptococcus pneumoniae culture-positive fluid from an infected joint.
Novocastra Polyomavirus
Clone 3.1.1
1 mL, 0.1 mL lyophilized Polyomavirus (JC/BK viruses)
NCL-JCBK IO RUO*
JC virus, a Polyomavirus of the Papovaviridae was first isolated from the
brain of an individual with a rare demyelinating condition. JC and BK viruses
are oncogenic in newborn hamsters and transform mammalian cells in vitro
but there is no evidence that they cause human cancer. JC virus is
ubiquitous in humans and may be occasionally shed in the urine without any
apparent symptoms during pregnancy or immunosuppression. Unlike BK
virus, also a Polyomavirus, JC virus may cause a lethal disease, progressive
multifocal leukoencephalopathy (PML). PML is a rare subacute
demyelinating disease of the CNS which is seen mainly as a complication of
advanced disseminated malignant conditions such as Hodgkin's disease or
chronic lymphocytic leukemia and also in HIV infection or following
immunosuppression for organ transplantation. The target cell of JC virus is
the oligodendrocyte in which the virus undergoes a lytic productive
infection. JC virus may be cultured from infected urine or brain in human
fetal glial cells or detected directly in biopsy or post-mortem tissue.
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.