QF0159 Marketing Release Record

Novocastra Serotonin
Polyclonal
0.5 mL lyophilized NCL-SEROTp P IVD
7 mL BOND ready-to-use PA0736 P (HIER)
Serotonin (5-hydroxytryptamine, 5-HT) is reported to be a widely distributed
neurotransmitter and hormone in the mammalian peripheral and central
nervous system (CNS). Serotonin is formed by the decarboxylation of 5hydroxy-tryptophan,
its intermediate, which in turn is formed by
hydroxylation of L-tryptophan by tryptophan hydroxylase. In the CNS, the
action of serotonin is terminated by reuptake into the presynaptic terminal
by specific serotonin transporters. Serotonin has been implicated in several
neuropsychiatric disorders such as anxiety, depression and schizophrenia.
The majority of serotonergic nerve terminals in the CNS originate in
neuronal cell bodies of the Raphé nuclei (dorsal, median), nucleus Raphé
obscurus and nucleus Raphé pallidus in the brainstem which project to
specific areas of the brain and spinal cord. Serotonin is thought to be an
inhibitory neurotransmitter regulating a wide range of sensory, motor and
cortical functions in the CNS. In the periphery, serotonin is reported to be
present in neural and non-neural structures such as platelets, gastrointestinal
tract (myenteric plexus, enterochromaffin cells), lungs
(neuroepithelial cells), thyroid gland and spleen.
Novocastra Sialyl Lewis a (CA19-9)
Clone C241:5:1:4
1 mL lyophilized NCL-CA19-9 F P (HIER)
1 mL liquid NCL-L-CA19-9 F P (HIER)
Refer to page 27 for further information about CA19-9.
Novocastra SM22 alpha
Clone 10H12
1 mL liquid NCL-L-SM22a P (HIER) W
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SM22 alpha, also known as smooth muscle cell specific protein, is reported
to be expressed at high levels in various tissues such as intestine, lung and
uterus. It shares structural homology with the regulatory protein, calponin.
SM22 alpha expression has been reported in most tissues that contain
smooth muscle in both vascular and visceral forms. It is also reported to be
expressed in some rare malignant tumors such as malignant fibrous
histiocytomas.
Human prostate: immunohistochemical staining for SM22 alpha using NCL-L-SM22a.
Note intense staining of smooth muscle. Paraffin section.
IVD
Novocastra SMA (Alpha Smooth Muscle
Actin)
Clone �sm-1
1 mL lyophilized NCL-SMA F P (Enzyme) W
7 mL ready-to-use RTU-SMA F P (Enzyme)
7 mL BOND ready-to-use PA0943 P IVD
Refer to page 21 for further information about Alpha Smooth Muscle Actin.
Novocastra SNAP-25
Clone SP12
0.5 mL lyophilized NCL-SNAP-25 P (HIER) W
The release of neurotransmitters from neurons is regulated by exocytosis of
synaptic vesicles. Exocytosis is mediated by a complex consisting of
membrane components of both the synaptic vesicle and the synaptic plasma
membrane. Synaptosomal-associated protein of 25 kD (SNAP-25) is a
plasmalemmal protein and is one of four proteins which are thought to make
up an initial docking complex for regulated exocytosis. SNAP-25 lacks a
transmembrane domain, but is linked to the membrane by palmitoylated
cysteine residues in the central region of the molecule. SNAP-25 has been
reported to be expressed in tumor cells of prolactinomas, growth hormone
secreting tumors and the granule cell layer and molecular layers of the
cerebellum.
Human small bowel: immunohistochemical staining for SNAP-25 using NCL-SNAP-25.
Note intense cytoplasmic staining of neural elements. Paraffin section.
Novocastra Spectrin Antibodies
Clone RBC2/3D5
1 mL, 0.1 mL lyophilized Spectrin (recommended for human
use) NCL-SPEC1 FWE IVD
Clone RBC1/5B1
1 mL lyophilized Spectrin (broad spectrum) NCL-SPEC2 F
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Antigen Background
Spectrin is a cytoskeletal protein which has some structural homology with
dystrophin, the protein that is defective in Duchenne and Becker muscular
dystrophy. Subtle membrane damage frequently occurs during the excision
and freezing of muscle samples. Labeling for spectrin is necessary to monitor
membrane integrity. It is reported that fibers which show negative labeling for
both dystrophin and spectrin are damaged (or in the early stages of
regeneration), whereas fibers which are negative for dystrophin but positive
for spectrin reflect true abnormalities of dystrophin expression.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
IVD
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Primary Antibodies