QF0159 Marketing Release Record

Human aorta: immunohistochemical staining for elastin using NCL-ELASTIN. Note extracellular
staining within the arterial wall. Paraffin section.
Novocastra Emerin
Clone 4G5
1 mL, 0.1 mL lyophilized NCL-EMERIN F P (HIER) W
Antigen Background
Emery-Dreifuss muscular dystrophy (EDMD) is a late onset X-linked
recessive disorder characterized by slowly progressing contractures,
wasting of skeletal muscle and cardiomyopathy usually presented as heart
block. Contractures are seen in the elbows, Achilles tendons and
postcervical muscles with humero-peroneal distribution early in the course
of the disease. The STA gene, at Xq28 locus, encodes a serine-rich 34 kD
protein, emerin, which is ubiquitous in tissues and is found in highest
concentration in skeletal and cardiac muscle. Emerin is localized in the
nuclear membrane of normal muscle cells and its deficiency plays a crucial
part in the pathology of EDMD.
Product Specific Information
NCL-EMERIN is of use in the detection of the normal STA gene product.
Human skeletal muscle: immunohistochemical staining for emerin using NCL-EMERIN.
Note perinuclear staining of all cell nuclei. Paraffin section.
Novocastra Endothelial Cell Marker
(CD34)
Clone QBEnd/10
1 mL, 0.1 mL lyophilized NCL-END F P (Enzyme) C
1 mL liquid NCL-L-END F P (Enzyme) C IVD
7 mL ready-to-use RTU-END F P (Enzyme) IVD
7 mL BOND ready-to-use PA0212 P (HIER) IVD
Refer to page 39 for further information about CD34.
IVD
IVD
Novocastra Endothelin-1 Receptor (ETA)
Clone RJT24
1 mL, 0.1 mL liquid NCL-L-ETA P (HIER)
Endothelins (ET) are potent vasoconstrictive peptides originally isolated
from vascular endothelial cells. Their biological effects are mediated
through two different receptors, endothelin-1-selective endothelin receptor
(ETA) and the non-selective receptor sub-type (ETB). Analysis of mRNA by
Northern blotting reported high levels in aorta, lung, atrium, colon and
placenta with moderate levels in the cerebral cortex, cerebellum, ventricle,
kidney, adrenal glands and duodenum, whereas liver was negative. Studies
using immunohistochemistry have reported the presence of ETA in medial
smooth muscle of arteries, full-term placenta, normal and diseased gall
bladder and ovarian luteinized granulosa cells. ETA has also been reported
to have a role in gastric ulcer healing and in the development of neural-crest
derived cells.
Human skin, pyogenic granuloma: immunohistochemical staining for endothelin-1 receptor
using NCL-L-ETA. Note membrane staining of the endothelial cells. Paraffin section.
Novocastra Enterovirus
RUO*
Clone 5-D8/1
1 mL lyophilized enterovirus (unconjugated)
NCL-ENTERO WIO RUO*
Enteroviruses are a large family of viruses whose main site of infection is the
alimentary tract. Dissemination via the bloodstream is the likely route of
spread to the wide range of target organs susceptible to infection. Most
enterovirus infections are subclinical in young children. However, they can
cause a wide range of syndromes involving many of the body systems eg
myocarditis, respiratory and neonatal diseases.
Product Specific Information
NCL-ENTERO recognizes an epitope on the VP1 peptide, which is highly
conserved within the Enterovirus group, except for Hepatitis A virus. The
antibody reacts with most echovirus strains (except some strains of echovirus
22 and 23), Poliovirus and Enterovirus strains. No reaction is observed with
tissue culture grown strains of Respiratory syncytial virus, Parainfluenza virus
types 1, 2, 3 and 4b, Herpesvirus types 1 and 2, Influenza virus types A and B,
Mumps virus, Measles virus, Varicella-zoster virus, Cytomegalovirus and
negative tissue culture cells routinely used in virus isolation.
Coxsackie B4 virus infected BW HEp cells: immunocytochemical staining for Coxsackie B4
virus using NCL-ENTERO. Note strong cytoplasmic staining of infected cells. Acetone-fixed
cells.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies