QF0159 Marketing Release Record

Human lymph node, follicular lymphoma; immunohistochemical staining for Bcl-6 using
NCL-L-Bcl-6-564. Note intense nuclear staining of malignant lymphocytes. Paraffin section.
Novocastra Bcl-10 Oncoprotein
Clone DAA22
1 mL lyophilized NCL-Bcl-10 W
Antigen Background
RUO*
Bcl-10 oncoprotein is an apoptotic regulatory molecule identified as a result
of its direct involvement in the translocation event t(1;14) (p22;q32) of mucosaassociated
lymphoid tissue (MALT) lymphomas. MALT lymphomas are the
most common subset of extranodal non-Hodgkin’s lymphomas. Wild type Bcl-
10 oncoprotein promotes apoptosis under normal circumstances and induces
NF-kappaB activation. Mutated forms of Bcl-10 oncoprotein have been found
to be associated with many common forms of cancer. The N-terminal region
of Bcl-10 oncoprotein encodes a caspase recruitment domain (CARD) which
is homologous with regions of several proteins involved in apoptosis. Several
mutation events lead to the Bcl-10 oncoprotein becoming truncated in the
region immediately downstream of the CARD. The truncated molecules do not
induce apoptosis and gain a transforming function that is not present in the
full length molecule. These truncated molecules are, therefore, thought to be
important factors that encourage cell proliferation during tumor
development. In normal tissues, Bcl-10 oncoprotein is expressed only in
breast and lymphoid tissues with staining predominantly found in the
cytoplasm.
Product Specific Information
NCL-Bcl-10 is raised to the majority of the coding region of the Bcl-10
oncoprotein.
Western blot: detection of Bcl-10 oncoprotein (32 kD) using NCL-Bcl-10. Lane A, molecular
weight markers. Lane B, A549 cell line immunoblotted with NCL-Bcl-10.
Novocastra Bcl-w
Clone 6C1
1 mL lyophilized NCL-Bcl-w P (HIER) W
Bcl-w belongs to the Bcl-2 family of proteins and promotes cell survival,
whereas other members such as bak and bax are antagonists and promote
apoptosis. The Bcl-w gene is highly conserved between mice and man. Bclw
protein is reported to be found in a diverse range of tissues including
cerebellum, hippocampus, colon, liver, heart, stomach, skeletal muscle,
testis and placenta. It is also expressed in most myeloid and a few lymphoid
cell lines including those of macrophage megakaryocytic and erythroid
origin. It is not expressed on B and T cell lines. Bcl-w is apparently
dispensable in normal development and function of most organs but is
essential for spermatogenesis.
Human brain, normal adult cerebellum: immunohistochemical staining for Bcl-w protein using
NCL-Bcl-w. Note intense cytoplasmic staining of Purkinje cells and their processes. Paraffin
section.
Novocastra bcl-x
Clone NC1
1 mL lyophilized NCL-bcl-x FP
RUO*
RUO*
Bcl-x has homology with and is a member of the Bcl-2 family of proteins.
Bcl-x can function as a regulator of cell death independently of bcl-2.
Differential splicing of the bcl-x mRNA produces short and long variants
known as bcl-x s and bcl-x L . These variants have different functions. Bcl-x
immunoreactivity has been demonstrated in many cell types and like bcl-2,
has been localized to the cytosol associated with mitochondria. Bcl-x has
been demonstrated to be immunohistochemically detected in plasma cells,
activated lymphocytes in interfollicular areas and a small number of
lymphocytes within germinal centers. It has also been reported in Reed
Sternberg cells in about 86 percent of Hodgkin's disease cases. In normal
tissues, bcl-x expression has been reported in cortical thymocytes,
megakaryocytes, red blood cell precursors and some types of differentiating
myeloid cells in bone marrow as well as spermatocytes and spermatids in
the testes. It is also found in mammary epithelial cells, secretory and basal
epithelial cells of the prostate, gastrointestinal epithelial cells and
differentiated keratinocytes in the upper layers of the epidermis (but not in
basal cells).
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies