QF0159 Marketing Release Record

Primary Antibodies
Novocastra Dystrophin Antibodies
Clone Dy4/6D3
2.5 mL, 1 mL lyophilized Dystrophin (Rod Domain)
NCL-DYS1 FWE IVD
Clone Dy8/6C5
2.5 mL, 1 mL lyophilized Dystrophin (C-terminus)
NCL-DYS2 FWE IVD
Clone Dy10/12B2
2.5 mL, 1 mL lyophilized Dystrophin (N-terminus)
NCL-DYS3 FWE IVD
Clone 13H6
1 mL lyophilized Dystrophin (C-terminus)
NCL-DYSA P (HIER) RUO*
Clone 34C5
1 mL lyophilized Dystrophin (N-terminus)
NCL-DYSB P (HIER) RUO*
Antigen Background
Duchenne Muscular dystrophy (DMD) is the most severe of the muscular
dystrophies resulting in progressive muscular wasting and death. Dystrophin
is the 427 kD protein product of the DMD/BMD gene located on the X
chromosome at position Xp2. Western blotting and immunohisto-chemistry
are the two established methods for the detection of abnormalities of
dystrophin expression in muscle samples.
Product Specific Information
NCL-DYS1, NCL-DYS2 and NCL-DYS3 map within amino acids 1181-1388,
3669-3685 and 321-494, respectively, on the dystrophin molecule. The
immunolabeling patterns for NCL-DYS1, NCL-DYS2 and NCL-DYS3 are similar.
NCL-DYSA is raised to a region of the dystrophin molecule, upstream from the
C-terminal region and NCL-DYSB is raised to a region of the N-terminus of the
dystrophin molecule. NCL-DYSA and NCL-DYSB will be of particular interest
in the investigation of archived formalin-fixed, paraffin-embedded material.
Labeling with an antibody to beta-spectrin, to monitor membrane integrity, is
an essential immunohistochemical control.
Human skeletal muscle: immunohistochemical staining for dystrophin using NCL-DYSA. Note
membrane staining of normal muscle fibers (A) and reduced and variable staining of muscle
fibers in an individual with Duchenne and Becker muscular dystrophy (B). Paraffin section.
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Novocastra E-Cadherin
Clone 36B5
1 mL, 0.1 mL lyophilized NCL-E-Cad P (HIER)
7 mL ready-to-use RTU-E-Cad P (HIER) IVD
7 mL BOND ready-to-use PA0387 P (HIER)
Antigen Background
E-cadherin is a Ca 2+ -dependent, transmembrane cell adhesion molecule. It
plays an important role in the growth, development and the intercellular
adhesion of epithelial cells. Most tumors have an abnormal architecture and
any subsequent loss of adhesiveness is thought to be an important step in the
development of local invasion. E-cadherin may have a role in neoplastic
progression, particularly as a suppressor of invasion. In prostate cancers, for
example, the expression of E-cadherin is reported to be reduced or absent in
comparison with its expression in normal prostate which is uniformly strong.
Reduced expression or absence of E-cadherin in addition to alpha, beta and
gamma-catenin in primary breast carcinomas has also been reported and
these four proteins are associated with the development of metastases.The
main immunohistochemical utility of E-cadherin is to highlight differential
expression of this protein in lobular and ductal carcinomas.
Human pulmonary adenocarcinoma: immunohistochemical staining for E-cadherin using
NCL-E-Cad. Note intense cytoplasmic and membrane staining of tumor cells. Paraffin section.
Novocastra Elastin
Clone BA-4
0.5 mL lyophilized NCL-ELASTIN P (Enzyme)
Elastin is a polymeric protein found in connective tissue which imparts the
property of elasticity to vertebrate elastic tissue. It is synthesized and
secreted as a soluble, single-chain protein (tropoelastin) which undergoes a
number of post-ribosomal modifications prior to its organization into an
elastic fiber in the extracellular space. Once secreted, tropoelastin
molecules are joined covalently via chemical modification and cross-linking
of specific lysyl residues to form the mature insoluble elastin.
Ultrastructurally, it is predominantly an amorphous material which may
change its morphology with ageing and different disease states. The
abnormal accumulation of elastic tissue in blood vessels is found in
atherosclerosis and hypertension. Genetic defects in the elastin molecule
are reported to lead to inherited diseases such as Marfan's syndrome,
pseudoxanthoma elasticum and the Bushke-Ollendorf syndrome.
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
IVD
IVD
RUO*