QF0159 Marketing Release Record

Human prostatic adenocarcinoma: immunohistochemical staining for androgen receptor using
NCL-AR-318. Note nuclear staining of tumor cells. Paraffin section.
Novocastra APC (Adenomatous
Polyposis Coli Protein)
Clone EMM43
1 mL lyophilized NCL-APC P
The human adenomatous polyposis coli (APC) gene at locus 5q21 encodes a
protein of 2,843 amino acids. A precise role for APC in the regulation of the
wnt/beta-cateninin signalling pathway has been clearly recognized. APC
forms molecular complexes which are able to eliminate intra-cytoplasmic
beta-catening, inducing its degradation. It is expressed in the cytoplasm of
epithelial and mesenchymal cell types. In the epithelium of bladder, small
and large intestine, esophagus, stomach and epidermis, APC expression is
restricted to regions in which cell replication has ceased and terminal
differentiation has been established. Expression has been reported in lung,
kidney and mammary gland endothelial, myoepithelial and duct lining
epithelial cells. Some tissues such as ovary, myometrium, thyroid,
parathyroid and tonsil do not express the protein. Mutations of the APC gene
have been linked to the development of sporadic colorectal tumors, as well
as familial adenomatous polyposis and cancers of the pancreas, stomach
and esophagus. APC mutations have also been observed at significantly
high frequency in the advanced stages of breast cancer suggesting a
biological role in carcinogenesis.
Human small intestine: immunohistochemical staining for adenomatous polyposis coli protein
using NCL-APC. Note cytoplasmic staining of intestinal epithelial cells. Paraffin section.
IVD
Novocastra Apoptosis Protease
Activating Factor 1
Polyclonal
1 mL lyophilized NCL-APAF1 F P (HIER)
Apoptosis is one of a number of responses that may occur as a result of
signal transduction pathways in the cell. One identified mechanism for
initiating caspase activation requires the participation of mitochondria and
involves a 130 kD protein known as apoptosis protease activating factor-1
(Apaf-1). Apaf-1 is a cytosolic protein that remains in a latent state until
bound to cytochrome c (Apaf-2). Cytochrome c is commonly released from
the mitochondria during apoptosis induced by many, but probably not all cell
death stimuli. The resulting Apaf1/cytochrome c complex associates with
the zymogen form of caspase-9 (Apaf-3) in the presence of dATP or ATP,
promoting the autocatalytic activation of caspase-9. Once activated
caspase-9 can then cleave and activate procaspase-3 directly, resulting in a
cascade of additional caspase activation and apoptosis.
Novocastra Aurora Kinase 2
Clone JLM28
1 mL liquid NCL-L-AK2 ASR
Analyte Specific Reagent. Analytical and performance characteristics are
not established.
Novocastra B Cell Marker (MB2)
Clone MB2
1 mL lyophilized NCL-MB2 FP
RUO*
MB2 is a pan B cell marker that is expressed in all B cells except mature
plasma cells. It does not react with T cells. These include epidermis (but
excludes the squamous cell layer), epithelia of breast, lung, pancreas,
stomach, colon, bladder, fallopian tube and also hepatocytes and stromal
cells of the ovary. MB2 has been reported to react with an uncharacterized
cytoplasmic antigen found in both normal B cells and B cell lymphomas.
Human tonsil: immunohistochemical staining for B lymphocytes using NCL-MB2. Note intense
cytoplasmic staining of normal B lymphocytes. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
/23
Primary Antibodies