QF0159 Marketing Release Record

Buffers Epitope Retrieval Solutions
Antibody Diluent
500 mL RE7133 FPO
Novocastra IHC Diluent is intended for use as a diluent for Novocastra
primary antibodies, Novocastra Concentrated Biotinylated Secondary
Antibody, RE7108, and Novocastra Concentrated Streptavidin-HRP, RE7109,
in immunohistochemical (IHC) procedures. Novocastra IHC Diluent is not
intended for the reconstitution of lyophilized reagents. Volume supplied is
500 mL.
Normal Serum Reagents
Blocking Reagent
10 mL Normal Goat Serum NCL-G-SERUM FP
10 mL Normal Horse Serum NCL-H-SERUM FP
10 mL Normal Rabbit Serum NCL-R-SERUM FP
IVD
RUO*
RUO*
RUO*
Normal serum is often used as a negative control or as a blocking reagent in
immunoassays. These may be of use as ‘no primary' controls and as a
diluent for primary and secondary antibody reagents. Novocastra offers
these animal sera in a convenient 10 mL pack size. 200 mL of working
strength diluent can be prepared, sufficient for up to 2000 slides.
Epitope Retrieval Solutions pH6
1 L pH6 (x10 Concentrate) RE7113 P (HIER) IVD
500 mL pH6 (x10 Concentrate) RE7114 P (HIER)
Novocastra Epitope Retrieval Solutions are intended for Heat Induced
Epitope Retrieval (HIER) on formalin-fixed, paraffin-embedded tissue
sections as part of an immunohistochemical procedure. HIER using an
appropriate pH solution improves the staining of some antibodies by
exposing epitopes within tissue that has been masked during fixation. The
development of Epitope Retrieval using heat was first reported in 1991 by Shi
S-R et al., Journal of Histochemistry and Cytochemistry 39: 741-748 (1991).
Since then numerous studies have been published looking at the effects of
molarity, pH and heating methods on epitope retrieval. A universal HIER
technique suitable for all epitopes does not exist. A combination of different
heating methods and epitope retrieval solutions may be used to optimize
unmaskingof antigens where this technique is recommended. HIER is not
recommended for all antibodies. Optimum conditions for epitope retrieval
should be validated by the user, as these are dependant upon tissue, fixation
and/or primary antibody. RE7113 is supplied as a 1 L volume, sufficient to
prepare 10 L of working solution. RE7114 is supplied as a 500 mL volume,
sufficient to prepare 5Lofworking solution.
Colonic adenocarcinoma pre-treated with Epitope Retrieval Solution pH6 (RE7113). Staining for
Cytokeratin 20 protein using NCL-L-CK20-561. Paraffin section.
Epitope Retrieval Solutions pH8
1 L pH8 (x10 Concentrate) RE7116 P (HIER)
Novocastra Epitope Retrieval Solutions are intended for Heat Induced
Epitope Retrieval (HIER) on formalin-fixed, paraffin-embedded tissue
sections as part of an immunohistochemical procedure. HIER using an
appropriate pH solution improves the staining of some antibodies by
exposing epitopes within tissue that has been masked during fixation. The
development of Epitope Retrieval using heat was first reported in 1991 by Shi
S-R et al., Journal of Histochemistry and Cytochemistry 39: 741-748 (1991).
Since then numerous studies have been published looking at the effects of
molarity, pH and heating methods on epitope retrieval. A universal HIER
technique suitable for all epitopes does not exist. A combination of different
heating methods and epitope retrieval solutions may be used to optimize
unmaskingof antigens where this technique is recommended. HIER is not
recommended for all antibodies. Optimum conditions for epitope retrieval
should be validated by the user, as these are dependant upon tissue, fixation
and/or primary antibody. RE7116 is supplied as a 1 L volume, sufficient to
prepare 10 L of working solution.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
IVD
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Buffers