QF0159 Marketing Release Record

Primary Antibodies
Novocastra p53 Protein (CM5)
Polyclonal
0.2 mL lyophilized NCL-p53-CM5p P (HIER) W
Antigen Background
The accumulation of p53 protein in response to genotoxic stress in vitro is
well established and appears to induce growth arrest and apoptosis by the
transcriptional regulation of other genes and possibly by other direct
mechanisms.
Product Specific Information
NCL-p53-CM5p is specific for mouse and rat p53 protein.
Western blot: detection of p53 protein (53 kD) using NCL-p53-CM5p. Lane A, molecular weight
markers. Lane B, T3T3 mouse cell line immunoblotted with NCL-p53-CM5p.
Novocastra p53 Protein (DO-1)
Clone DO-1
1 mL lyophilized NCL-p53-DO1 F P (HIER) W
In man, the p53 gene is located on the small arm of chromosome 17.
Alterations of the p53 tumor suppressor gene are a common feature of
human malignancies. A normal function of this gene is to induce apoptosis
after DNA damage and, therefore, its activation can permit the survival of
cells that have sustained genetic damage.
Product Specific Information
Clone DO-1 recognizes both wild type and mutant forms of human p53 protein
under denaturing and non-denaturing conditions. The epitope to which NCLp53-DO1
maps is sited at the N-terminus at amino acids 20 – 25.
Human colonic adenocarcinoma: immunohistochemical staining for p53 protein using
NCL-p53-DO1. Note intense nuclear staining of tumor cells. Paraffin section.
/92
For detailed information on all products please visit our website:
www.leica-microsystems.com
RUO*
RUO*
Novocastra p53 Protein (DO-7)
Clone DO-7 Reference Range
1 mL, 0.1 mL lyophilized NCL-p53-DO7 FPWC
1 mL liquid NCL-L-p53-DO7 FPWC IVD
7 mL ready-to-use RTU-p53-DO7 FP IVD
7 mL BOND ready-to-use PA0057 P (HIER)
Product Specific Information
IVD
This monoclonal antibody recognizes both wild type and mutant forms of
human p53 protein under denaturing and non-denaturing conditions. The
epitope recognized by clone DO-7 can be destroyed by prolonged fixation in
buffered formalin. The heat induced epitope retrieval technique may improve
staining in some cases.
Human skin, basal cell carcinoma: immunohistochemical staining for p53 protein using the
ready-to-use reagent RTU-p53-DO7. Note intense nuclear staining of tumor cells. Paraffin
section.
Novocastra p57 Protein (Kip2)
Clone 25B2
1 mL lyophilized NCL-p57 P (HIER)
See also Kip2 (p57 Protein) on page 76.
Novocastra p73 Protein (alpha)
Clone 24
1 mL lyophilized NCL-p73 F P (HIER)
p73 protein was the first identified homolog of the tumor suppressor gene,
p53. Overproduction of p73 protein reported in p53-defective tumor cells,
activates p53-responsive promoters. This results in the induction of
apoptosis but its function in tumor development is unclear. Alternative
splicing produces at least six known p73 mRNA species resulting in p73
isoforms; alpha, beta, gamma, delta, epsilon and zeta. The relative
expression level of each splice variant may modulate p73 transcriptional and
growth suppression activity. p73 protein expression is reported to be low in
normal tissues eg normal squamous epithelium. Elevated expression has
been shown by RT-PCR and/or western blotting in a number of tumors
including approximately 40 percent of breast carcinomas, 80 percent of lung
tumors, 50 percent of ovarian tumors and 30 percent of hepatocellular
carcinomas.
IVD
RUO*
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
IVD