QF0159 Marketing Release Record

Human tonsil: immunohistochemical staining for human neutrophil defensins using NCL-
DEFENSIN. Note intense granular cytoplasmic and extracellular staining of neutrophils.
Paraffin section.
Novocastra Human Securin
Clone DCS-280.2
1 mL lyophilized NCL-SECURIN P (HIER)
Human securin (hsecurin), also known as pituitary tumor-transforming gene-
1 (PTTG) product, is required for chromosomal stability in human cells.
Abnormalities of chromosome number are reported to be amongst the most
common genetic aberrations in cancer. The mechanisms for regulating
mitotic chromosome transmission in mammalian cells are, therefore, of
great interest. Human cells without an hsecurin gene lose chromosomes at
a high rate. These losses have been linked to abnormal anaphases during
which cells undergo repeated unsuccessful attempts to segregate their
chromosomes. Therefore, human securin is essential for the maintenance of
euploidy. The expression of hsecurin is reported to correlate with cell
proliferation in a cell cycle-dependent manner in both normal tissues and in
several tumor types. hsecurin specifically binds to Ku, the regulatory subunit
of the DNA-dependent protein kinase. Ku and hsecurin associate both in
vitro and in vivo. DNA double-strand breaks prevent Ku/hsecurin association
showing that genome damaging events can result in the induction of
pathways that activate DNA repair mechanisms and halt cell cycle
progression. It has also been proposed that hsecurin connects DNAdamage
response pathways with sister chromatid separation delaying
mitosis while DNA repair occurs.
Human tonsil: immunohistochemical staining for human securin using NCL-SECURIN.
Note nuclear staining of proliferating cells. Paraffin section, nickel DAB.
Novocastra Human Spasmolytic
Polypeptide
Clone GE16C
1 mL lyophilized NCL-HSP P (HIER)
RUO*
RUO*
Human spasmolytic polypeptide (HSP) is a member of the trefoil peptide
family which is reported to be expressed in discrete regions of the body,
most notably the gastrointestinal tract. In the stomach, HSP is reported to be
localized to foveolar and surface epithelium, pyloric glands and mucous
neck cells.
Normal human stomach: immunohistochemical staining of human spasmolytic polypeptide
using NCL-HSP. Note cytoplasmic staining of cardiac glands. Paraffin section.
Novocastra Human von Willebrand
Factor (Factor VIII-related antigen)
Clone 36B11
1 mL, 0.1 mL lyophilized NCL-vWF F P (HIER)
1 mL liquid NCL-L-vWF F P (HIER) IVD
7 mL BOND ready-to-use PA0400 P (HIER)
Antigen Background
Human von Willebrand factor (or factor VIII-related antigen) is a 270 kD
multimeric plasma glycoprotein. It mediates platelet adhesion to injured
vessel walls and serves as a carrier and stabilizer for coagulation factor VIII.
The von Willebrand factor has functional binding domains to platelet
glycoprotein Ib, glycoprotein Ib/IIIa, collagen and heparin. von Willebrand
factor is synthesized by endothelial cells and is reported to be expressed in a
number of tumors of vascular origin.
Novocastra Hypoxia Inducible Gene 2
Protein
Clone HX34Y
1 mL, 0.1 mL liquid NCL-L-HIG2 P (HIER)
Antigen Background
The gene encoding hypoxia-inducible gene 2 protein (HIG2) is one of the
transcriptional targets for the activated beta-catenin/Tcf-4 complex and its
product functions as an autocrine growth factor that enhances cell growth.
This gene encodes a trans-membrane protein of 7 kD molecular weight that
was found to be expressed exclusively in renal cell carcinomas (RCC) and
fetal kidney. Reports indicate that ELISA analysis of clinical samples
identified secretion of HIG2 protein into plasma of RCC patients even at an
early stage of tumor development. HIG2 expression is reported to be
expressed at higher levels in ovarian clear cell carcinomas when compared
to those of clear cell renal tumors.
IVD
RUO*
Human clear cell renal cell carcinoma: immunohistochemical staining for hypoxia inducible
gene 2 protein using NCL-L-HIG2.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
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Primary Antibodies