QF0159 Marketing Release Record

Primary Antibodies
Novocastra Troponin Antibodies
Clone 1A2
1 mL lyophilized troponin C NCL-TROPC P
Clone T1/61
1 mL lyophilized troponin C NCL-TROPT F P (Enzyme) W
Troponin comprises three protein subunits, troponin C, troponin I and
troponin T. It is a contractile protein which comprises 5 percent of muscle
proteins. Troponin C, an 18 kD protein, binds calcium and is responsible for
regulating the process of thin filament activation during skeletal muscle
contraction. Troponin I, a 21 kD protein, is the inhibitory subunit of the
complex and troponin T is responsible for binding the troponin subunits to
tropomyosin, a 66 kD protein that links the troponin complex to the actin
helix. The troponin C gene is reported to be expressed in three distinct
striated muscle lineages; cardiac myocytes, embryonic fast skeletal
myotubules and adult slow skeletal myocytes. Reports have indicated that
cardiac myofibers from cardiomyopathic rodent models display decreased
Ca 2+ sensitivity and that this property is a result of an alteration in the
troponin/tropomyosin regulatory complex in the fibers.
Product Specific Information
NCL-TROPT reacts with human and chicken fast muscle troponin, but not
slow muscle troponin T.
Human skeletal muscle: immunohistochemical staining for troponin T using NCL-TROPT.
Note intense staining of fast muscle fibers. Paraffin section.
Novocastra Tuberin
Clone 3B4
1 mL lyophilized NCL-TUBERIN P (HIER)
Tuberin is the 180 kD protein product of the tuberous sclerosis (TSC-2) gene
located on chromosome 16. Tuberous sclerosis is an autosomal disorder
known as phakomatosis which is characterized by the widespread development
of benign growths, usually described as hamartomata, in many tissues
and organs. Tuberin is widely expressed at low levels in most human
tissues, with increased expression in cortical neurons, cerebellar Purkinje
cells, motor neurons of the spinal cord, pancreatic islet B cells, cardiac
muscle and small blood vessels of the kidney and skin.
Human cerebellum: immunohistochemical staining for tuberin using NCL-TUBERIN.
Note cytoplasmic staining of Purkinje cells. Paraffin section.
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Novocastra Tubulin Beta II
Clone KNY-379
1 mL lyophilized NCL-TUB-B2 P (HIER) W
Tubulin beta II is one of four subunits of tubulin. Tubulin is one of the most
widespread classes of multiprotein families where alpha and beta subunits
are used in the construction of microtubules. Studies in rats report that
tubulin beta II represents a significant proportion of beta tubulin expressed
in the adrenal gland, brain and testis. In fetal tissues, this isoform has been
reported in skeletal muscle as well as in brain. Expression has also been
reported in migrating neuroblasts, peripheral nerves, ganglion cells and
sensory organs of the developing rat nervous system and in developing
skeletal and smooth muscle cells, chondrocytes and vascular endothelia.
Product Specific Information
Clone KNY-379 does not cross-react with vimentin or nestin.
Human neuroblastoma: immunohistochemical tubulin beta II lipoprotein receptor using
NCL-TUB-B2. Note intense cytoplasmic staining of malignant cells. Paraffin section.
Novocastra Tumor Necrosis Factor
Receptor-Associated Factor 1
Clone 7C11
1 mL liquid NCL-L-TRAF-1 P (HIER)
TNF receptor-associated factors (Traf) are a family of proteins that bind to
surface receptors forming signalling complexes with additional proteins that
mediate some cellular responses. Traf-1 can homodimerize or heterodimerize
with other Traf proteins leading to the activation of some
transcription factors such as nuclear factor kappa B and Jun-N-kinase. The
activation of nuclear factor kappa B is known to act in concert with
additional proteins to suppress TNF-alpha mediated apoptosis. The
expression of this protein is reported to be induced by Epstein-Barr Virus
(EBV).
Product Specific Information
IVD
NCL-L-TRAF-1 stains optimally when used in TBS-based wash buffer and
diluent systems.
Hodgkin’s Lymphoma: immunohistochemical staining for Traf-1 protein using NCL-L-TRAF-1.
Note intense membrane staining of Reed Sternberg cells. Paraffin section.
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
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