QF0159 Marketing Release Record

Human medullary thyroid carcinoma: immunohistochemical staining for calcitonin using
NCL-L- CALCITONIN. Paraffin section.
Novocastra Calpain
Clone Calp3c/11B3
2.5 mL lyophilized NCL-CALP-11B3 W
Clone Calp3c/12A2
2.5 mL, 1 mL lyophilized NCL-CALP-12A2 W
Antigen Background
RUO*
RUO*
At least seven forms of autosomal recessive muscular dystrophy (MD) have
been included under the banner “limb girdle muscular dystrophy” (LGMD).
These forms may be divided into two groups; those with abnormal expression
of the dystrophin/glycoprotein complex and those in which labeling of the
proteins in this complex is unaffected. Thus the sarcoglycanopathies (also
known as LGMD types 2C, 2D, 2E and 2F) are caused by defects in the genes
for gamma, alpha, beta and delta-sarcoglycan on chromosomes 13q12, 17q21,
4q12 and 5q33, respectively. Among the dystrophies in which expression of
the sarcoglycans is normal, the gene responsible for LGMD2A has been
identified as the chromosome 15q15-encoded muscle-specific calciumactivated
neutral protease, calpain 3. Calpain 3 enzyme is only stable in
human muscle when homogenized in treatment buffer immediately after
harvest. (Anderson LVB et al. American Journal of Pathology. 153(4): 1169-
1179 (1998)), and in homogenates containing SDS and is therefore well suited
for analysis by Western blot.
Product Specific Information
NCL-CALP-2C4 reacts with the full-size calpain 3 (94 kD) and an additional
fragment (30 kD) in human skeletal muscle. NCL-CALP-12A2 reacts with fullsize
protein plus apparent degradation products at approximately 60 kD.
Specificity of these antibodies has been confirmed by the loss of all these
bands in samples with null gene mutations. NCL-CALP-11B3 reacts with
calpain 3 bands at 94 and 60 kD, pre- and post-autolyzed forms of the
ubiquitous calpains 1 and 2 (� and m-calpain) staining a group of bands
between 76 and 84 kD in human skeletal muscle (Anderson LVB et al.
American Journal of Pathology. 153(4): 1169-1179, (1998)) Cross-reactivities in
different animals and tissues are described (see reference).
Western blot: analysis of human skeletal muscle showing detection of the calpain family of
proteins. Lane A, calpain 3 bands at 94 and 30 kD detected with NCL-CALP-2C4. Lane B,
calpains 1, 2 and 3 detected with NCL-CALP-11B3. Lane C, calpain 3 bands at 94 and
approximately 60 kD detected with NCL-CALP-12A2. Photograph supplied courtesy of Dr Louise
V B Anderson.
Novocastra Calponin (Basic)
Clone 26A11
1 mL lyophilized NCL-CALPONIN-B ASR
7 mL BOND ready-to-use PA0416 P (HIER) IVD
Analyte Specific Reagent. Analytical and performance characteristics are
not established.
Novocastra Calretinin (5A5)
Clone 5A5
1 mL, 0.1 mL lyophilized NCL-CALRETININ P (HIER)
1 mL liquid NCL-L-CALRETININ P (HIER) IVD
7 mL ready-to-use RTU-CALRETININ P (HIER) IVD
Antigen Background
Calretinin is an intracellular calcium-binding protein of 31.5 kD belonging to
the troponin C superfamily characterized by a structural motif described as
the EF-hand domain. Calcium is an important moderator of a number of vital
physiological processes, including neuronal excitability, axonal transport,
synthesis and release of some neurotransmitters, membrane permeability
and enzyme activity. Calretinin is found in the nervous system and thymus.
Calretinin can also be demonstrated in normal and neoplastic mesothelial
cells and has been reported to be a useful marker for the identification of
malignant mesotheliomas of epithelial type to differentiate these from
metastases of lung adenocarcinoma where antibodies to detect calretinin
are used in within a panel and interpretation together with clinical data is
undertaken by a qualified pathologist.
Human mesothelioma: immunohistochemical staining for calretinin using NCL-L-CALRETININ.
Note cytoplasmic staining of malignant cells. Paraffin section.
Novocastra Calretinin (CAL6)
Clone CAL6 Reference Range
1 mL, 0.1 mL liquid NCL-L-CALRET-566 P (HIER) W
7 mL BOND ready-to-use PA0346 P (HIER) IVD
Antigen Background
Calretinin is a calcium-binding protein of 29 kD that is a member of the family
of so-called EF-hand proteins that also includes S-100 proteins. Calretinin is
reported to be abundantly expressed in neurons. Outside the nervous system,
calretinin is reported to be expressed in a range of cell types including
mesothelial cells, steroid producing cell, (eg adrenal cortical cells, Leydig
cells, ovarian theca interna cells as well as Sertoli cells, some
neuroendocrine cells, eccrine sweat glands) and other cell types. The
presence of calretinin is reported to be a useful marker primarily for
differentiating malignant mesothelioma from carcinomas. Other reports
indicate the detection of calretinin is helpful for the differential
characterization of ovarian stroma tumors. Calretinin-positive cells have also
been reported in the convoluted tubules of kidney with some antibodies.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
IVD
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Primary Antibodies