QF0159 Marketing Release Record

Primary Antibodies
Human brain, cerebellum: immunohistochemical staining for alpha parvalbumin using NCL-
PARVALBUMIN. Note cytoplasmic staining of Purkinje, basket and stellate cells. Paraffin
section.
Novocastra Parvovirus B19
Clone R92F6
1 mL, 0.1 mL lyophilized NCL-PARVO FP
Antigen Background
Parvovirus B19 is a small, single-stranded DNA virus which causes erythema
infectiosum also known as ‘slapped cheek syndrome'. Clinically, this is a
febrile disease in children, often epidemic, with a facial maculopapular rash
causing flushed cheeks. In individuals with erythrocyte abnormalities, such
as sickle cell anaemia, Parvovirus B19 can cause hemolytic complications
where the virus replicates in bone marrow cells and inhibits erythropoiesis.
Parvovirus B19 has also been implicated with spontaneous abortion in
humans.
Product Specific Information
NCL-PARVO is specific for the viral antigens, VP1 (84 kD) and VP2 (58 kD).
Human fetal lung, post-mortem tissue: immunohistochemical staining for Parvovirus B19 using
NCL-PARVO. Note intense staining of infected cells within the capillaries. Paraffin section.
Novocastra Pax-5
Clone 1EW
1 mL liquid NCL-L-PAX-5 ASR
7 mL BOND ready-to-use PA0552 P (HIER) IVD
Analyte Specific Reagent. Analytical and performance characteristics are
not established.
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For detailed information on all products please visit our website:
www.leica-microsystems.com
RUO*
Novocastra P-Cadherin
Clone 56C1
1 mL lyophilized NCL-P-Cad F P (HIER) W
P-cadherin, like E-cadherin, is a Ca 2+ -dependent cell adhesion molecule
and has a fundamental role in maintaining the integrity of multicellular
structures. It is responsible for selective cell to cell adhesion. P-cadherin
expression is reported to be restricted and the protein is only detected in the
basal or parabasal layers of stratified epithelia. P-cadherin may contribute
to the maintenance of the epithelial phenotype and be involved in the final
stage of tumor progression in epidermal carcinomas. Changes in the pattern
of P-cadherin expression have also been reported in breast and melanocytic
cancers.
Novocastra PECAM-1 (CD31)
Clone 1A10
1 mL, 0.1 mL lyophilized NCL-CD31-1A10 P (HIER)
7 mL BOND ready-to-use PA0250 P (HIER) IVD
See also CD31 (PECAM-1) on page 38.
Novocastra Perforin
Clone 5B10
1 mL lyophilized NCL-PERFORIN P (HIER)
Antigen Background
Perforin is a pore-forming protein found in cytoplasmic granules of cytotoxic
T-lymphocytes (CTLs). CTLs bind to cells which express foreign antigens and
induce them to lyse. Perforin forms circular lesions on the target cell
membrane similar to those induced by complement. Perforin and C9 share a
high degree of homology particularly at the membrane spanning region.
Perforin is reported to be constitutively expressed in human CD3 negative,
CD56 positive NK cells, CD3 positive large granular lymphocytes and gamma/
delta T cells. This expression is significantly induced in CD8 positive T cells
but to a lesser extent in gamma/delta T cells and NK cells. The induction of
perforin mRNA is partially blocked by the immunosuppressive drug
cyclosporin A.
Human follicular lymphoma: immunohistochemical staining for perforin using NCL-PERFORIN.
Note focal granular staining of occasional cytotoxic T lymphocytes. Paraffin section.
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
RUO*
IVD
IVD