QF0159 Marketing Release Record

Novocastra CD10
Clone 56C6 Reference Range
1 mL, 0.1 mL lyophilized NCL-CD10-270 F P (HIER) W
1 mL liquid NCL-L-CD10-270 F P (HIER) W IVD
7 mL ready-to-use RTU-CD10-270 F P (HIER) IVD
7 mL BOND ready-to-use PA0270 P (HIER) IVD
Antigen Background
CD10 antigen, also called neprilysin, is a 100 kD cell surface
metalloendopeptidase which inactivates a variety of biologically active
peptides. It was initially identified as the common acute lymphoblastic
leukemia antigen (CALLA) and was thought to be tumor-specific. Subsequent
studies, however, have shown that CD10 antigen is expressed on the surface
of a wide variety of normal and neoplastic cells. In other lymphoid
malignancies, CD10 antigen is reported to be expressed on cells of
lymphoblastic, Burkitt's and follicular lymphomas. CD10 antigen has been
identified on the surface of normal early lymphoid progenitor cells, immature
B cells within adult bone marrow and germinal center B cells within lymphoid
tissue. It is also expressed in various non-lymphoid cells and tissues, such as
breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high
expression on the brush border of kidney and gut epithelial cells. (G.
McIntosh et al. American Journal of Pathology. 154(1): 77-82 (1999)).
Human lymphoblastic lymphoma: immunohistochemical staining for CD10 antigen using
NCL-CD10-270. Note intense membrane staining of neoplastic lymphoid cells. Paraffin section.
Novocastra CD11b (Mac-1)
Clone 44
1 mL lyophilized NCL-CD11b F
RUO*
The CD11b (Mac-1) leukocyte associated protein consists of two polypeptide
chains with molecular weights 95 kD and 170 kD. Mac-1 functions as
a cell surface receptor for the C3bi complement fragment. It belongs to a
family of heterodimeric proteins (designated CD11) which share a common
beta chain CD18 (95 kD). The alpha chain component (CD11b) of the
leukocyte associated protein is expressed on granulocytes, monocytes,
macrophages and NK cells.
IVD
Novocastra CD11c
Clone 5D11 Reference Range
1 mL, 0.1 mL liquid NCL-L-CD11c-563 P (HIER)
7 mL BOND ready-to-use PA0554 P (HIER)
Antigen Background
CD11c is a member of the leukocyte integrin family of adhesion proteins. It is
reported to be expressed in normal tissues, mainly on myeloid cells eg in bone
marrow myelocytes, premyelocytes, metamyelocytes, non-segmented and
segmented neutrophils with high levels reported on tissue macrophages and
monocytes and with lowest levels in granulocytes. It is also reported to be
expressed on NK cells, activated T cells, lymphoid cell lines, including hairy
cell leukemias and a proportion of interdigitating dendritic cells. CD11c
antigen expression is reported to be a useful indicator of monocyte
differentiation in the classification of acute myeloid leukemias and for the
identification of hairy cell leukemias.
Human hairy cell leukemia: immunohistochemical staining for CD11c antigen using
NCL-L-CD11c-563. Note membrane staining of malignant cells. Paraffin section.
Novocastra CD13
Clone 38C12
1 mL, 0.1 mL lyophilized NCL-CD13-304 P (HIER)
Antigen Background
CD13 antigen, also known as aminopeptidase N, is a member of the type II
integral membrane metalloproteases which also includes the leukocyte
antigens CD10, CD26, CD73 and BP-1. CD13 antigen is a receptor for the
coronaviruses which cause respiratory disease in humans and several
animal species. The antigen functions as a zinc-binding metalloprotease
which plays a role in cell surface antigen presentation by trimming the Nterminal
amino acids from MHC class II-bound peptides. CD13 antigen is
reported to be expressed on granulocytes, monocytes and their precursors,
most acute myeloid leukemias and a smaller proportion of acute lymphoid
leukemias. Non-hematopoietic cells which express CD13 antigen include
epithelial cells, renal proximal tubules, intestinal brush border, endothelial
cells, fibroblasts, brain cells, bone marrow, osteoclasts and cells lining the
bile canaliculi.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
IVD
IVD
IVD
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Primary Antibodies