QF0159 Marketing Release Record

Primary Antibodies
Novocastra CD81
Clone 1D6
1 mL lyophilized NCL-CD81 P (HIER)
CD81 antigen, also known as TAPA-1, is a member of the TM4 superfamily
and is predicted to have four transmembrane regions, short cytoplasmic
N and C-termini and two extracellular regions. CD81 protein has a molecular
weight of 26 kD and is expressed by most cell types. Of the hematopoietic
cells, CD81 protein is reported to be expressed by B and T cells,
macrophages, dendritic cells, NK cells and eosinophils but not by
neutrophils, platelets or erythrocytes. The CD81 protein associates noncovalently
with a number of other molecules eg CD19, CD21, MHC class
I and II, CD20, CD37, CD53 and CD82 in B cells and CD4, CD8 and CD82 in
T cells. It also associates with the integrins CD29/CD49c (VLA-3), CD29/
CD49d (VLA-4) and CD29/CD49f (VLA-6) in several other cell types. No
extracellular ligand has been identified for CD81 protein and its function
remains unclear, although mouse CD81 protein plays a role in early T cell
development. The human CD81 molecule has been reported to be involved in
cell adhesion, motility, metastasis as well as cell activation and signal
transduction.
Human tonsil: immunohistochemical staining for CD81 antigen using NCL-CD81. Note
membrane staining of most cell types including B and T lymphocytes and vascular endothelial
cells. Paraffin section.
Novocastra CD82
Clone 5B5
1 mL lyophilized NCL-CD82 P (HIER)
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CD82 antigen, also known as KAI1 or C33 antigen, is a member of the TM4
superfamily. It is expressed in most cell types, including B and T cells,
NK cells, monocytes, granulocytes and platelets but not in erythrocytes.
Upon lymphocyte activation, CD82 antigen expression is reported to be
strongly upregulated and, in vitro, it can transduce signals in B cells, T cells
and monocytes. The expression of CD82 antigen is reported to suppress
metastasis in tumor cells. In benign prostatic hyperplasia, the expression of
CD82 antigen was found to be uniform in the cellular membrane of glandular
epithelial cells. However, tissue from untreated prostate cancer showed
more variable expression.
Human skin, squamous cell carcinoma: immunohistochemical staining for CD82 antigen using
NCL-CD82. Note intense membrane staining of tumor cells. Paraffin section.
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Novocastra CD83
Clone 1H4b
1 mL lyophilized NCL-CD83 P (HIER)
CD83 antigen, a member of the immunoglobulin superfamily, is reported to
be expressed on mature and activated dendritic cells. These include
Langerhans cells in the skin, peripheral blood dendritic cells and
interdigitating reticulum cells within the T cell zones of lymphoid organs. In
early human pregnancy, decidua is reported to contain immunostimulatory
CD83 antigen positive dendritic cells. CD83 antigen is reported to be
expressed in Hodgkin's disease and can be found to be expressed in most
Reed Sternberg cells. In breast carcinoma, mature CD83 positive cells may
be found in peripheral areas amongst T cells, which resembles dendritic/T
cell clusters of secondary lymphoid organs. This is a characteristic of
ongoing immune reactions where mature and activated dendritic cells are
essential for the recruitment of activated tumor specific lymphocytes during
carcinogenesis. Some germinal center B cells and activated peripheral
lymphocytes also express CD83 antigen.
Hodgkin's disease, mixed cellularity type: immunohistochemical staining for CD83 antigen
using NCL-CD83. Note membrane staining and characteristic paranuclear hofs of Reed
Sternberg cells. Paraffin section.
Novocastra CD95 (Fas)
Clone GM30
1 mL lyophilized NCL-FAS-310 F P (HIER)
See also Fas (CD95) on page 63.
Novocastra CD99
Clone PCB1
1 mL, 0.1 mL liquid NCL-L-CD99-187 P (HIER)
Clone 12E7
7 mL BOND ready-to-use PA0509 P
Antigen Background
CD99 is a 32 kDa transmembrane glycoprotein, encoded by the MIC2 gene, which
is located in the pseudoautosomal region of the human X and Y chromosomes.
Recently, the MIC2 gene has been shown to encode two distinct proteins which
are produced by alternative splicing of the CD99 gene transcript and are
identified as bands of 30 and 32 kDa (p30/32).
Although its function is not fully understood, CD99 has been implicated in various
cellular processes including homotypic aggregation of T cells, upregulation of T
cell receptor and MHS molecules, apoptosis of immature thymocytes and
leukocyte diapedesis. CD99 is reported to be expressed on most human tissues
including cortical thymocytes, pancreatic islets cells, Leydig and Sertoli cells,
virtually all hematopoietic cell types (except granulocytes), peripheral blood
lymphocytes, granulose cells of the ovary, endothelial cells and basal/parabasal
squamous epithelial cells. CD99 expression has been reported in a wide range of
tumors, including Ewing’s sarcoma and T cell lymphoma.
IVD
IVD
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
RUO*
IVD