QF0159 Marketing Release Record

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Buffers Epitope Retrieval Solution pH9 1 L pH9 (x10 Concentrate) RE7119 P (HIER) 1 L pH9 (x10 Concentrate) RE7224 P (HIER) Novocastra Epitope Retrieval Solutions are intended for Heat Induced Epitope Retrieval (HIER) on formalin-fixed, paraffin-embedded tissue sections as part of an immunohistochemical procedure. HIER using an appropriate pH solution improves the staining of some antibodies by exposing epitopes within tissue that has been masked during fixation. The development of Epitope Retrieval using heat was first reported in 1991 by Shi S-R et al. Journal of Histochemistry and Cytochemistry 39: 741-748 (1991). Since then numerous studies have been published looking at the effects of molarity, pH and heating methods on epitope retrieval. A universal HIER technique suitable for all epitopes does not exist. A combination of different heating methods and epitope retrieval solutions may be used to optimize unmasking of antigens where this technique is recommended. HIER is not recommended for all antibodies. Optimum conditions for epitope retrieval should be validated by the user, as these are dependant upon tissue, fixation and/or primary antibody. RE7119 and RE7224 are supplied as a 1 L volume, sufficient to prepare 10 L of working solution. Kidney pre-treated with Epitope Retrieval Solution pH9 (RE7119). Staining for Wilms' tumor protein using NCL-L-WT1-562. Paraffin section. / 122 For detailed information on all products please visit our website: www.leica-microsystems.com IVD IVD Enzyme Proteinase K (IHC) 100 mL kit RE7160-K P (Enzyme) Enzyme pretreatment of formalin-fixed, paraffin-embedded tissue sections improves the staining of some antibodies by exposing epitopes within tissue that have been masked during fixation. The first proteolytic enzyme employed for epitope retrieval was trypsin. More recently, proteinase K which is commonly used in in situ hybridization techniques has been reported to be of use. Product Specific Information Novocastra Enzyme Proteinase K (IHC), RE7160-K, is intended for the enzymatic pretreatment of formalin-fixed, paraffin-embedded tissue sections prior to incubation with a primary antibody in an immunohistochemical (IHC) procedure. This product can be used for epitope retrieval with Novocastra antibodies for which trypsin is recommended, known exceptions to this are NCL-C-JEJUNI, NCL-BrdU, NCL-CYCLIN D1, NCL-COLL-IIp, and NCL-CYCLIN D1-GM. This two part kit comprises 0.75 mL of Enzyme Proteinase K Concentrate, RE7126, and 100 mL of Enzyme Proteinase K Buffer, RE7127, sufficient to produce 100 mL of working strength enzyme solution. This product is used in an IHC procedure, which allows the qualitative identification by light microscopy. Epitope retrieval by enzymatic pretreatment is recommended for a limited number of antibodies. Optimum conditions for epitope retrieval should be validated by the user as these are dependent upon tissue, fixation and/or primary antibody. IVD Liver pre-treated with Enzyme Proteinase K (RE7160-K). Staining for Cytokeratin 8/18 using NCL-L-5D3. Paraffin section. Products in this catalog are subject to regulatory approval. Please consult your Leica Microsystems representative for availability in your region. * For Research Use Only. Not for use in diagnostic procedures.
ISH Reagents/ Probes NovocastraTM ISH Reagents/Probes The Novocastra ISH probe range includes Fluorescein-Conjugated Oligonucleotide probes for the qualitative detection of RNA transcripts. F Frozen I Immunofluorescence E Electron microscopy P Paraffin C Flow cytometry O Other applications W Western blotting / 123 ISH Reagents/Probes
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Reagent products are designed prima
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Contents Total Histology Solutions
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Routine R Staining and a Coverslipp
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IS NOW OPEN! Over 1000 Novocastra a
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Up to 50% faster Three additional r
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BOND Leica BOND Reagents and Consum
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Ancillary Reagents BOND Wash Soluti
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Consumables BOND Aspirating Probe C
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PrimaryAntibodies NovocastraTM Prim
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Name Clone Product Code Anaplastic
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Novocastra Alpha-1-Antitrypsin Poly
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Human prostatic adenocarcinoma: imm
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Human lymph node, follicular lympho
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Novocastra BL-CAM (CD22) Clone FPC1
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Human medullary thyroid carcinoma:
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Novocastra Caspase-9 Clone 2C9B11 1
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Human small intestine, T cell lymph
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Novocastra CD10 Clone 56C6 Referenc
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Human bone marrow: immunohistochemi
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Human glomangioma: immunohistochemi
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Human squamous cell carcinoma, floo
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Novocastra CD69 Clone CH11 1 mL lyo
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Human primitive neuroectodermal tum
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Novocastra CD163 Clone 10D6 1 mL ly
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Human insulinoma: immunohistochemic
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Product Specific Information Please
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Novocastra Cytokeratin 7 Clone RN7
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Novocastra Cytokeratin 20 Clone PW3
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Novocastra Deleted in Pancreatic Ca
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Human aorta: immunohistochemical st
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Novocastra Epstein-Barr virus (LMP-
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Novocastra Factor XIIIa (Blood Coag
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Novocastra Folylpolyglutamate Synth
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Novocastra Glial Fibrillary Acidic
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Novocastra Gross Cystic Disease Flu
Page 73 and 74: Novocastra HLA Class II (DR) Antige
Page 75 and 76: Human tonsil: immunohistochemical s
Page 77 and 78: Novocastra Immunoglobulin M Clone 8
Page 79 and 80: Novocastra Lambda Light Chain Clone
Page 81 and 82: Novocastra Mast Cell Chymase Clone
Page 83 and 84: Novocastra Mesothelin Clone 5B2 1 m
Page 85 and 86: Human Colonic Carcinoma: immunohist
Page 87 and 88: Novocastra Multidrug Resistance- As
Page 89 and 90: Novocastra Myosin Heavy Chain Antib
Page 91 and 92: Novocastra Nitric Oxide Synthase An
Page 93 and 94: Novocastra p21 (WAF1 Protein) Clone
Page 95 and 96: Normal human cervix: immunohistoche
Page 97 and 98: Novocastra Peripherin Clone PJM50 1
Page 99 and 100: Product Specific Information NCL-JC
Page 101 and 102: Novocastra P-selectin (CD62P) Clone
Page 103 and 104: Novocastra Serotonin Polyclonal 0.5
Page 105 and 106: Human cerebellum: immunohistochemic
Page 107 and 108: Product Specific Information NCL-L-
Page 109 and 110: Novocastra Topoisomerase II Alpha C
Page 111 and 112: Novocastra Tyrosinase Clone T311 1
Page 113 and 114: Novocastra Villin Clone CWWB1 1 mL,
Page 115 and 116: ManualDetection NovocastraTM Manual
Page 117 and 118: DAB Enhancer 25 mL RE7125 PO Novoca
Page 119 and 120: Miscellaneous Biotin (Hapten Antibo
Page 121 and 122: Buffers NovocastraTM Epitope Retrie
Page 123: Buffers Epitope Retrieval Solutions
Page 127 and 128: Human Herpesvirus (type 8) Probe (F
Page 129 and 130: ASR Probes NovocastraTM ASR Probes
Page 131 and 132: Primer Sets NovocastraTM Primer Set
Page 133 and 134: Corynebacterium diphtheriae 100 Tes
Page 135 and 136: Origin Origin Familiar and trusted
Page 137 and 138: CD3 Clone PS1 50 Tests ORG-8982 Ant
Page 139 and 140: CD79a Clone 11E3 50 Tests ORG-8975
Page 141 and 142: Ki67 Clone MM1 50 Tests ORG-8772 An
Page 143 and 144: Terminal Deoxynucleotidyl Transfera
Page 145 and 146: Power- Vision PowerVisionTM The fre
Page 147 and 148: PowerVision Poly-HRP anti-Mouse IHC
Page 149 and 150: Product Name Index Product Name Ind
Page 151 and 152: Novocastra Product Code Page Unit 3
Page 153 and 154: Product Code Page Unit CD2 (LFA-2)
Page 155 and 156: Product Code Page Unit CD45RO NCL-U
Page 157 and 158: Product Code Page Unit Cytokeratin
Page 159 and 160: Product Code Page Unit Fascin NCL-F
Page 161 and 162: Product Code Page Unit Laminin NCL-
Page 163 and 164: Product Code Page Unit Nitric Oxide
Page 165 and 166: Product Code Page Unit Retinoblasto
Page 167 and 168: Origin Product Code Page Unit bcl-2
Page 169: Leica Microsystems operates globall
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QF0159 Marketing Release Record

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