QF0159 Marketing Release Record

Novocastra HLA Class II (DR) Antigen
Clone LN-3
1 mL lyophilized NCL-LN3 FP
HLA-DR is an MHC Class II antigen that maps to chromosome 6. It is a
heterodimer composed of 2 non-covalently associated glycoproteins of
about 35 kD (alpha, heavy) and 27 kD (beta, light). Both chains are comprised
of two Ig-like domains and have transmembrane sequences and short
cytoplasmic tails. It is reported to be expressed mainly on antigenpresenting
cells (monocytes/macrophages and dendritic cells), B cells and
some activated T cells. Expression has also been reported on thymic
epithelial cells.
Human anaplastic lymphoma: immunohistochemical staining for HLA class II antigen using
NCL-LN3. Note membrane staining of large cells. Paraffin section.
Novocastra HMB45 (Melanoma Marker)
Clone HMB45 Reference Range
1 mL, 0.1 mL liquid NCL-L- HMB45 F P (Enzyme)
7 mL BOND ready-to-use PA0027 P (Enzyme)
Product Specific Information
The HMB45 antigen has also been identified in retinal pigment epithelium
(RPE) but is reported to be reactive only with the transient prenatal and
infantile RPE. No reaction is reported to be observed with intradermal nevi
and normal adult melanocytes and non-melanocytic cells. Tumor cells of
epithelial, lymphoid, glial and mesenchymal origin are reported to be
negative. This clone is well described in the literature. It is indicated to label
an intracytoplasmic antigen in the majority of melanomas and other tumors
demonstrating melanoma/melanocytic differentiation. The clone is also
reported to react with junctional and blue nevus cells. (Bacchi CE et al., A
Review. Applied Immunohistochemistry. 4:73-85 (1996)).
Human metastatic melanoma: immunohistochemical staining for melanoma cells using
NCL-L-HMB45. Note cytoplasmic staining of malignant cells. Paraffin section
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Novocastra Human Chorionic
Gonadotrophin (alpha)
Clone 4E12
1 mL lyophilized NCL-HCG-alpha F P (HIER)
The human chorionic gonadotrophin alpha (hCGa) gene has now been
identified as an estrogen receptor alpha (ERa) responsive gene in breast
cancer cells. It encodes the common alpha subunit of the four secreted
glycoprotein hormones, hCG, LH, FSH and TSH. The common alpha chain
and the hormone-specific beta chains have molecular weights of 14 kD and
17 kD, respectively. hCGa is expressed as part of hCG in normal placenta
and as part of LH, FSH and TSH in the pituitary gland. hCGa mRNA is
reported to be detected in normal pregnant women and in the peripheral
blood mononuclear cells of patients with trophoblastic disease. Additionally,
hCG is reported to be detected in seminomatous and non-seminomatous
testicular cancers as well as in its free alpha and beta subunits.
Independent studies suggest that approximately half of ERa positive breast
cancers express hCGa.
Normal human placenta: immunohistochemical staining for human chorionic gonadotrophin
alpha using NCL-HCG-alpha. Note cytoplasmic staining of syncytiotrophoblasts. Paraffin
section.
Novocastra Human Chorionic
Gonadotrophin (beta)
Polyclonal
1 mL lyophilized NCL-HCGp F P (Enzyme)
7 mL BOND ready-to-use PA0014 P (HIER)
Human chorionic gonadotrophin (hCG) is a glycoprotein hormone produced
by trophoblastic cells of the placenta beginning 10 to 12 days after
conception. Maintenance of the fetus in the first trimester of pregnancy
requires the production of hCG, which binds to the corpus luteum of the
ovary which is stimulated to produce progesterone which in turn maintains
the secretory endometrium. hCG is composed of two subunits, alpha and
beta. The alpha subunit of hCG is identical to the subunit of luteinising
hormone, thyroid stimulating hormone and follicle stimulating hormone. The
common alpha chain and the hormone-specific beta chains have molecular
weights of 14 kD and 17 kD, respectively. The hCG beta-subunit is unique in
the family of beta-containing glycoprotein hormones in that it contains an
extension of 29 amino acids at its COOH end. It is believed that the Cterminal
region of the HCG-beta subunit plays a role in the intracellular
behavior of the heterodimer.
Product Specific Information
NCL-HCGp was raised to the isolated beta-chain of human chorionic
gonadotrophin and reacts with placental trophoblasts. NCL-HCGp shows a
slight cross-reaction with luteinising hormone and may, therefore, stain
basophil cells in the pituitary.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies