QF0159 Marketing Release Record

Primary Antibodies
Human squamous cell carcinoma: immunohistochemical staining for cytokeratin 1 using
NCL-CK1. Note intense cytoplasmic staining of tumor cells. Paraffin section.
Novocastra Cytokeratin 4
Clone 6B10
0.5 mL lyophilized NCL-CK4 F P (HIER) W
Cytokeratin 4 is a 59 kD cytokeratin intermediate filament protein. It is found
in non-cornifying squamous epithelium such as that of the superficial and
intermediate epithelial cells of the esophagus, ectocervix, tongue, vagina,
larynx, pharynx, epiglottis, anus as well as the superficial cells of the
cornea. Cytokeratin 4 is also reported to be expressed in the suprabasal
cells of the urinary bladder transitional epithelium, in single cells and cell
groups of sweat glands, prostatic ducts and in cylindrical, ciliated bronchial
epithelial cells. Cytokeratin 4 is reported to be in squamous cell carcinomas
derived from several non-cornified stratified epithelia.
Product Specific Information
NCL-CK4 is a chain-specific antibody. It is of particular use in the characterization
of certain complex epithelia.
Human tonsil: immunohistochemical staining for cytokeratin 4 using NCL-CK4.
Note cytoplasmic staining of mucosal epithelium. Paraffin section.
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RUO*
Novocastra Cytokeratin 5
Clone XM26 Reference Range
1 mL, 0.1 mL lyophilized NCL-CK5 F P (HIER) W
1 mL liquid NCL-L-CK5 F P (HIER) W IVD
7 mL ready-to-use RTU-CK5 F P (HIER) IVD
7 mL BOND ready-to-use PA0468 P (HIER) IVD
Antigen Background
Cytokeratins are a large family of cytoskeletal proteins found in epithelial cells.
They are coordinately synthesized in pairs so that at least one member of each
family is expressed in each epithelial cell. Cytokeratins assemble into obligatory
heteropolymers composed of type I (acidic) and type II (basic) polypeptides to
form higher order tetramers and protofilaments. Basal cells of human epidermis
express acidic keratin 14 and basic cytokeratin 5. Cytokeratin 5 is a 58 kD protein
that is closely related to cytokeratin 6. They share similar tissue distribution and
are found in various proportions in many non-keratinizing stratified squamous
epithelia eg tongue mucosa, as well as in basal epithelia of trachea, basal cells
of epidermis, hair follicles, sebaceous and sweat glands of skin, luminal cells of
the mammary gland, basal cells of prostate, urothelium, vagina and
endocervical mucosa. Cytokeratins 5 and 6 are also expressed in basal cell
epitheliomas, squamous cell carcinomas of skin, tongue, epiglottis and of the
rectal-anal region. Point mutations in the cytokeratin 5 gene at locus 12q11-q13
can cause various types of epidermolysis bullosa simplex. Cytokeratin 5 is also
reported to be expressed in most epithelial and biphasic mesotheliomas. Clone
XM26 is specific for the 58 kD intermediate filament protein known as
cytokeratin 5. It is not cross-reactive with cytokeratin 6.
Human prostate: immunohistochemical staining for cytokeratin 5 using NCL-L-CK5.
Note intense cytoplasmic staining of myoepithelial cells. Paraffin section.
Novocastra Cytokeratin 6
Clone LHK6B
1 mL lyophilized NCL-CK6 F IVD
Cytokeratins are precisely
regulated in tissue and little is known about the molecular mechanisms
underlying this regulation. However, the expression pattern of cytokeratin 6
is known to be particularly complex. It is found in hair follicles, suprabasal
cells of a variety of internal stratified epithelia, in epidermis, in both normal
and hyperproliferative situations. Epidermal injury results in activation of
keratinocytes which produce and respond to growth factors and cytokines
and become migratory. Activated keratinocytes express a specific pair of
cytokeratins, 6 and 16. Furthermore, cytokeratin 6 is reported to be
expressed in approximately 76 percent of head and neck squamous cell
carcinomas. The expression of cytokeratin 6 has been particularly
associated with differentiation.
Product Specific Information
NCL-CK6 reacts with the human cytokeratin intermediate filament protein
(56 kD) identified as cytokeratin 6.
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
IVD