QF0159 Marketing Release Record

Primary Antibodies
Novocastra Beta-2-Microglobulin
Polyclonal
1 mL lyophilized NCL-B2Mp P (Enzyme) O
Beta-2-microglobulin, a single polypeptide chain of molecular weight 11.6
kD, is present on the surface of most nucleated cells and its expression may
be decreased or lost in malignancy. Beta-2-microglobulin is the major
constituent of a subtype of secondary amyloidosis which is associated with
long term hemodialysis. Clinical and pathological features of this disease
have been characterized. Spontaneous fractures and destructive arthropathies
(articular swelling and pain in an oligoarticular distribution, along
with effusions in large joints) have been related to amyloid deposition.
Amyloid has been implicated in most clinical complaints of beta-2microglobulin-related
amyloid arthropathy where it is found in synovial
biopsies taken from the involved joints.
Product Specific Information
NCL-B2Mp is also effective in ELISA techniques.
Novocastra Beta Amyloid
Clone 6F/3D
1 mL lyophilized NCL-B-Amyloid FP
Antigen Background
Beta amyloid is an extracellular filamentous protein deposit found in the
brain. It is the major protein component of amyloid cores and neuritic plaques
and is also found as a deposit in neurofibrillary tangles. In man, Alzheimer's
disease is the most common cause of senile dementia and is characterized
by abnormal filamentous protein deposits in the brain. Beta amyloid deposits
are also detected in Lewy body dementia, Down's syndrome, amyloidosis
(Dutch type) and in the Guam Parkinson-Dementia complex.
Product Specific Information
Pretreatment of tissue sections with 98 to 100 percent formic acid is
recommended when using NCL-B-Amyloid.
Human brain, Alzheimer's disease: immunohistochemical staining for beta amyloid protein
using NCL-B-Amyloid. Note intense staining of senile plaques. Paraffin section.
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Novocastra Beta-Catenin
Clone 17C2
1 mL lyophilized NCL-B-CAT F P (HIER) W
7 mL BOND ready-to-use PA0083 P (HIER)
Antigen Background
The catenins, (alpha, beta and gamma) are cytoplasmic proteins which bind
to the highly conserved tail of the E-cadherin molecule. Beta-catenin is a
component of the adherens junction, a multiprotein complex which supports
Ca2+ -dependent cell to cell contact which in itself is critical for adhesion,
signal transmission and for anchoring the actin cytoskeleton. Beta-catenin’s
role is as a transcription effector of the wnt-signalling pathway. Immunohistochemistry
is the best way to demonstrate nuclear expression of betacatenin
and wnt-pathway activation. This aberrant expression is observed in
human tumorigenesis and especially in colorectal cancer.
Human endometrial adenocarcinoma: immunohistochemical staining for beta-catenin using
NCL-B-CAT. Note membrane staining of tumor cells. Paraffin section.
Novocastra Beta-Dystroglycan
Clone 43DAG1/8D5
1 mL, 0.1 mL lyophilized NCL-b-DG FWE
Antigen Background
Dystrophin associated glycoproteins (DAGs) are a complex of at least seven
proteins involved in the attachment of dystrophin to muscle membranes. The
biological significance of this dystrophin/glycoprotein complex is not fully
understood, but it appears to form an essential linkage between actin on the
inside of the muscle fiber and muscle laminin in the basal lamina which
surrounds the fiber. Beta-dystroglycan spans the muscle membrane and it
has been suggested that it is the member of the complex which binds directly
to dystrophin. Labeling of beta-dystroglycan may be reduced in some forms
of muscular dystrophy where another component eg dystrophin or laminin, is
directly affected. Labeling with an antibody to beta-spectrin to monitor
membrane integrity, is an essential immunohistochemical control.
Western blot: detection of human beta-dystroglycan (43 kD) using NCL-b-DG. Lane A,
molecular weight markers. Lane B, human skeletal muscle extract immunoblotted with
NCL-b-DG.
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
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