QF0159 Marketing Release Record

Primary Antibodies
Novocastra Collagen Type VI (�3 Chain)
Clone 64C11
1 mL lyophilized NCL-COLL-VI P (HIER)
Normal human placenta: immunohistochemical staining for collagen type VI using
NCL-COLL-VI. Note pericellular staining of cytotrophoblasts. Paraffin section
Novocastra Collagen Type VII
Clone LH7.2
1 mL lyophilized NCL-COLL-VII F
/50
RUO*
Collagen type VII is a basement membrane component which is the major
protein in the anchoring fibrils projecting from the lamina densa into the
subjacent connective tissue. Collagen type VII has been reported to be
detected in the basal lamina of stratified epithelia such as epidermis, oral,
oesophageal and cervical epithelium and urothelium of the bladder. Those
epithelia which are composed of different cell types eg sweat gland
epithelium or breast epithelium which are made up of myoepithelial cells
next to glandular cells, possess a type VII collagen-containing basement
membrane. Basement membranes play an important role in tumor
progression. In normal breast tissue, benign breast lesions and in situ
malignancies, the basement membrane always surrounds ducts and tubules
whereas in invasive breast carcinomas it is often absent. Collagen type VII is
reported to be a defective membrane component in the condition Recessive
Dystrophic Epidermolysis Bullosa (RDEB).
Human skin: immunohistochemical staining for collagen type VII using NCL-COLL-VII.
Note staining of the basal lamina of the stratified epithelium. Frozen section.
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RUO*
Novocastra Complement Component C9
Clone 10A6
1 mL lyophilized NCL-CCC9 P (HIER)
Complement component C9 binds to the C5b-8 complex as the final protein
of the membrane attack complex. After binding, it undergoes a conformational
change and inserts itself into the cell membrane, forming
transmembrane channels. Complement component C9 acts in a similar way
to perforin, a pore forming protein found in cytotoxic T cells. Male and
female reproductive tissues express and synthesize complement
components, binding proteins and receptors, although the implications of
this is unclear. The detection of complement component C9 has been
reported in cases of acute myocardial damage at necropsy. Detection of
myocardial infarction or diffuse damage can be unreliable with conventional
methods of examination of the heart such as enzyme histochemistry or by
the elaborate technique of quantification of contraction band necrosis.
Human myocardium: immunohistochemical staining for complement component C9 using
NCL-CCC9. Note staining of partially necrotic myocardium and vessel walls. Paraffin section.
Novocastra CPP32 (Caspase-3)
Clone JHM62
1 mL lyophilized NCL-CPP32 P (HIER) W
Cysteine protease protein (CPP)-32 is a member of the interleukin-1 betaconverting
enzyme (ICE) family of mammalian proteases which specifically
cleaves substrates at the C-terminal side of aspartic acid residues.
Members of this family have been implicated in apoptosis and CPP32
(caspase-3) is thought to act as a control mediator of programmed cell
death in mammalian cells. CPP32 is synthesized as an inactive 32 kD
proenzyme and is processed during apoptosis to its active form which is
responsible for the cleavage of poly (ADP-ribose) polymerase (PARP), actin
and sterol regulatory element binding protein (SREBP). CPP32 is reported to
be found in epithelial cells of skin, renal proximal tubules and collecting
ducts, epithelioreticular cells of the thymus and bronchial, colonic and
salivary duct epithelia. Chondrocytes, bone osteocytes, megakaryocytes,
mature neutrophils of bone marrow and plasma cells of the tonsil, lymph
node and bone marrow are also reported to express CPP32 antigen.
Novocastra Cyclin B1
RUO*
Clone 7A9
1 mL lyophilized NCL-CYCLIN B1 P (HIER) W C
Cyclin B protein acts in a similar way to cyclin A, as regulatory subunits of
p34/cdc2/cdk1 affecting the G2 to M phase transition. Cyclin B expression is,
therefore, restricted to a specific short period of the cell cycle with cyclin B1
expression detected earlier and peaking in concentration before cyclin B2
expression. Cyclin B positive cells, indicated by cytoplasmic staining, in
proliferating tissue are reported to represent a subset of Ki67 positive cells.
Products in this catalog are subject to regulatory approval.
Please consult your Leica Microsystems representative for availability in your region.
* For Research Use Only. Not for use in diagnostic procedures.
IVD
RUO*