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QF0159 Marketing Release Record

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Primary Antibodies<br />

Human seminoma: immunohistochemical staining for Oct-3/4 transcription factor using NCL-L-<br />

Oct-3/4. Note intense staining of pluripotent tumor cells. Paraffin section.<br />

Novocastra Osteonectin<br />

Clone 15G12<br />

1 mL, 0.1 mL lyophilized NCL-O-NECTIN P (HIER) W<br />

Osteonectin (ON), also known as BM-40 or SPARC (secreted protein, acidic<br />

and rich in cysteine) is a multifunctional glycoprotein (32.5 kD) involved with<br />

tissue mineralization as well as extracellular matrix modelling. ON is the<br />

most abundant glycoprotein secreted by human osteoblasts in developing<br />

bone and odontoblasts of developing teeth. ON mRNA and protein have<br />

been reported to be expressed in non-mineralized tissues such as steroidproducing<br />

cells of the adrenal glands, suprabasal layers of the epidermis,<br />

glomeruli in the kidney, bronchi of the lung, megakaryocytes and large<br />

vessels. This organ-specific distribution of ON in non-mineralized tissues<br />

suggests a role during human development. It is also reported to be<br />

expressed in osteosarcomas, at high levels in osteoblastic osteosarcomas<br />

and at low levels in osteoid formation of the diffuse deposition type.<br />

Human osteosarcoma: immunohistochemical staining for osteonectin using NCL-O-NECTIN.<br />

Note cytoplasmic staining of malignant cells. Paraffin section.<br />

/90<br />

For detailed information on all products please visit our website:<br />

www.leica-microsystems.com<br />

RUO*<br />

Novocastra Osteopontin<br />

Clone OP3N<br />

1 mL lyophilized NCL-O-PONTIN P (HIER)<br />

Osteopontin is a 34 kD extracellular matrix protein with a cell binding<br />

domain. Other molecules which share this domain include fibronectin,<br />

vitronectin and a variety of other extracellular proteins that bind members of<br />

the integrin family of cell surface receptors. Osteopontin was originally<br />

identified as a major component of the non-collagenous organic bone<br />

matrix, however, it has subsequently been demonstrated in a wide range of<br />

normal adult tissues and body fluids. It is a multifunctional protein involved<br />

in bone mineralization, cell adhesion, cell migration, chronic inflammatory<br />

disease and transformation. Osteopontin is reported to be linked to<br />

tumorigenesis and metastasis in several experimental animal models and<br />

human cancers. In breast carcinomas, demonstrated by RT-PCR and in situ<br />

hybridization studies, expression was confined to tumor cells. It is also<br />

reported to be expressed in normal breast, including vascular endothelial<br />

cells, macrophages, myoepithelial cells, osteosarcomas but not in lymphoid<br />

tumors. Other studies using in situ hybridization have shown expression in<br />

the epithelium of gastrointestinal tract, gall bladder, pancreas, urinary and<br />

reproductive tracts, lung, salivary and sweat glands. Ganglion cells in the<br />

bowel also express osteopontin as do macrophages, T cells and NK cells<br />

upon activation. Expression of osteopontin in vascular smooth muscle and<br />

endothelium may be triggered by atherosclerosis, vascular calcification and<br />

by hypertension.<br />

Human osteosarcoma: immunohistochemical staining for osteopontin using NCL-O-PONTIN.<br />

Note extracellular staining in close proximity to tumor cells. Paraffin section.<br />

Novocastra Ovarian Cancer Antigen<br />

(CA125)<br />

Clone Ov185:1<br />

1 mL lyophilized NCL-CA125 F P (HIER)<br />

1 mL liquid NCL-L-CA125 F P (HIER) IVD<br />

7 mL ready-to-use RTU-CA125 F P (HIER)<br />

7 mL BOND ready-to-use PA0539 P IVD<br />

See also CA125 (Ovarian Cancer Antigen) on page 28.<br />

Novocastra OX40 (CD134)<br />

Clone 102H6<br />

1 mL lyophilized NCL-CD134 F P (HIER)<br />

See also CD134 (OX40) on page 45.<br />

Products in this catalog are subject to regulatory approval.<br />

Please consult your Leica Microsystems representative for availability in your region.<br />

* For Research Use Only. Not for use in diagnostic procedures.<br />

IVD<br />

IVD<br />

IVD<br />

IVD

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