QF0159 Marketing Release Record

Novocastra Deleted in Pancreatic
Cancer Locus 4 Protein
Clone JM56
1 mL lyophilized NCL-DPC4 P (HIER)
Deleted in pancreatic cancer locus 4 (DPC4) is a tumor suppressor gene
reported to be frequently mutated or deleted in pancreatic and metastatic
colon cancers. DPC4, also known as Smad4, acts as a cofactor that binds
transforming growth factor-beta receptor-activated Smad2 and Smad3
generating transcriptional complexes which translocate to the nucleus to
participate in sequence-specific DNA-binding and transcriptional activation.
Mutation or deletion of the DPC4 gene is reported in 50 percent of
pancreatic ductal adenocarcinomas and a subset of acute myelogenous
leukemias, biliary tract carcinomas, ovarian, colon and breast cancers. The
expression of DPC4 protein has been reported to be a sensitive and specific
marker for DPC4 gene alterations in pancreatic carcinomas. Loss of DPC4
expression occurs late in the neoplastic progression which leads to the
development of infiltrating pancreatic cancer when it is histologically
recognizable as a carcinoma. The continued expression of DPC4 protein is
reported in pancreatic intraductal papillary mucinous neoplasms (IPMN)
and suggests genetic differences in tumorigenesis from ductal carcinomas.
Novocastra Desmin
Clone DE-R-11
1 mL lyophilized NCL-DES-DERII F P (Enzyme) W
1 mL liquid NCL-L-DES-DERII F P (Enzyme) W
7 mL ready-to-use RTU-DES-DERII F P (Enzyme)
7 mL BOND ready-to-use PA0032 P (HIER)
Product Specific Information
NCL-DES-DERII reacts with an 18 kD rod piece of the intermediate filament
protein desmin (53 kD) in muscle cells. The antibody does not appear to
recognize other intermediate filament proteins. In normal tissues, Clone DE-
RII reacts with both striated (skeletal and cardiac) and smooth muscle cells.
The labeling is confined to the Z bands in skeletal and cardiac muscle giving
a characteristic striated appearance.
Normal human small intestine: immunohistochemical staining for desmin using
NCL-DES-DERII. Note cytoplasmic staining of muscle cells in the muscularis externa.
Paraffin section.
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Novocastra DOG-1
Clone K9 Reference Range
1 mL, 0.1 mL liquid NCL-L-DOG-1 P (HIER)
7 mL BOND ready-to-use PA0219 P (HIER)
Antigen Background
DOG-1, a 986 amino acid protein of unknown function, is expressed
predominantly on the plasma membrane of gastrointestinal stromal tumors
(GISTs) and is rarely expressed in other soft tissue tumors, which, due to
appearance, can be confused with GISTs. Immunoreactivity for DOG-1 has
been reported to be found in 97.8 percent of scorable GISTs, including all KIT
negative GISTs. Reactivity for DOG-1 has been suggested to aid in the
identification of GISTs, including Platelet-Derived Growth Factor Receptor
Alpha mutants that fail to express KIT antigen.
Product Specific Information
The use of PBS-based diluents may result in increased background staining.
Human gastrointestinal stromal tumor: immunohistochemical staining for DOG-1 using
NCL-L-DOG-1. Note intense membrane and cytoplasmic staining of tumor cells. Paraffin
section.
Novocastra Dysferlin
Clone Ham1/7B6
1 mL, 0.1 mL lyophilized NCL-Hamlet FPW
Clone Ham3/17B2
1 mL lyophilized NCL-Hamlet-2 F P (HIER) W
Antigen Background
Dysferlin is the protein product of the 2p13 gene that is defective in individuals
with Limb-Girdle Muscular Dystrophy type 2B (LGMD2B) and Miyoshi
Myopathy (MM). Dysferlin is normally localized to the muscle plasma
membrane. In individuals with LGMD2B and MM, immunoreactivity to
dysferlin is reported to be severely reduced or lost, depending on the type of
mutation. Individuals with other neuromuscular conditions demonstrate
normal labeling patterns.
Product Specific Information
NCL-Hamlet may require heat induced epitope retrieval in some cases.
labeling with an antibody to beta-spectrin, to monitor membrane integrity, is
an essential immunohistochemical control.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies