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Chapter – 3 Preparation of small library…..
3.4.6 GENERAL PROCEDURE FOR THE PREPARATION OF DNJ-1301
TO DNJ-1305
To a stirred solution of 0.01 mole of 2-propylpentanohydrazide in 50 ml
warm ethanol was added 0.01 mole of an appropriate N-Mannich base of
isatin in 60 ml ethanol. Then 2–3 drops of glacial acetic acid were added and
the reaction mixture was refluxed for 5 hours. The progress and the
completion of the reaction were checked by silica gel-G F254 thin layer
chromatography using toluene : ethyl acetate (7 : 3) as a mobile phase. After
the reaction to be completed, the flask was cooled to afford a solid product.
The solid residue was filtered, washed with cold ethanol and dried.
Recrystallization was carried out from hot ethanol. (Physical data of the
synthesized end products are summarized in the table 3.5.1)
3.4.7 GENERAL PROCEDURE FOR THE PREPARATION OF DNJ-1401
TO DNJ-1405
It was again prepared by the method described by Chohan et. al. f To a
stirred solution of 0.01 mole of 2-amino benzothiazole in 50 ml warm ethanol
was added 0.01 mole an appropriate Mannich base of isatin in 60 ml ethanol.
Then 2–3 drops of concentrated sulphuric acid were added and the reaction
mixture was refluxed for 4 hours. The progress and the completion of the
reaction were checked by silica gel-G F254 thin layer chromatography using
hexane : ethyl acetate (6 : 4) as a mobile phase. After the reaction to be
completed, the flask was cooled to afford a solid product. The solid residue
was filtered, washed with cold ethanol, then with ether and dried.
Recrystallization was carried out from hot ethanol. (Physical data of the
synthesized end products are summarized in the table 3.5.2)
Department of Chemistry, Saurashtra University, Rajkot – 360 005 157