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Chapter – 6 Biological evaluation of newly.....
The representative compounds synthesized in earlier chapters were
evaluated for biological activity. The biological screening protocols and in
silico study of some of the compounds are reported in this chapter.
6.1 ANTIMICROBIAL SCREENING
Antibiotic resistance is a serious concern worldwide as it would result in
strains against which currently available antibacterial agents will be
ineffective. In general, bacterial pathogens may be classified as either grampositive
or gram-negative pathogens. Antibiotics compounds with effective
activity against both gram-positive and gram-negative pathogens are
generally regarded as having a broad spectrum of activity. The synthesized
compounds were preliminary screened against gram-positive and gramnegative
pathogens.
Gram-positive pathogens, for example Staphylococci, Enterococci,
Streptococci and Micobacteria bacteria are particular because of the
development of resistant strain which is difficult to eradicate from the hospital
environment once established. Example of such strains are methicillin
resistance Staphylococcus (MRSA), methicillin resistance coagulase negative
Staphylococci (MRCNS), penicilline resistance Streptococcus pnumaniae and
multiplied resistance Enterococcus faecium, community acquired pathogens
(CAP) and so on.
6.1.1 MINIMUM INHIBITORY CONCENTRATION (MIC)
In microbiology, MIC is the lowest concentration of an antimicrobial that
will inhibit the visible growth of a microorganism after overnight incubation.
Minimum inhibitory concentrations are important in diagnostic laboratories to
confirm resistance of microorganisms to an antimicrobial agent and also to
monitor the activity of new antimicrobial agents. An MIC is generally regarded
as the most basic laboratory measurement of the activity of an antimicrobial
agent against an organism.
Department of Chemistry, Saurashtra University, Rajkot – 360 005 327