Mayo Test Catalog, (Sorted By Test Name) - Mayo Medical ...

FBART
91439
Currently, histology results are considered the gold standard for diagnosing esophageal dysplasia and
adenocarcinoma. However, there are many limitations of biopsy including limited sampling of the
affected area, lengthy procedure time for biopsy collection, poor interobserver reproducibility of
pathologists to diagnose dysplasia or adenocarcinoma, and an inability of histologic findings to predict
patient progression from Barrett's esophagus to esophageal adenocarcinoma.(1) FISH, a technique that
utilizes fluorescently labeled DNA probes to examine cells for chromosomal alterations, can be used to
detect cells with chromosomal changes (eg, polysomy) that are indicative of neoplasia (dysplasia or
adenocarcinoma). Studies indicate that a multicolor, multitarget FISH assay that utilizes probes to 8q24
(MYC), 9p21 (CDKN2A), 17q12 (ERBB2; alias HER-2), and 20q13.2 (ZNF217), is able to detect
dysplasia and adenocarcinoma in endoscopic esophageal brushing specimens collected from patients with
Barrett's esophagus.(1,2)
Useful For: Aid to identifying dysplasia and adenocarcinoma in patients with Barrett's esophagus
Interpretation: An interpretive report is provided based on the combination of routine cytology and
FISH results.
Reference Values:
An interpretive report will be provided.
Clinical References: 1. Brankley SM, Wang KK, Harwood AR, et al: The development of a
fluorescence in situ hybridization assay for the detection of dysplasia and adenocarcinoma in Barrett's
esophagus. J Mol Diagn 2006 May;8(2):260-267 2. Rygiel AM, Milano F, Ten Kate FJ, et al: Gains and
amplifications of c-myc, EGFR, and 20q13 loci in the no dysplasia-dysplasia-adenocarcinoma sequence
of Barrett's esophagus. Cancer Epidermiol Biomarkers Prev 2008;17:1380-1385 3. Barr Fritcher EG,
Brankley SM, Kipp BR, et al: A comparison of conventional cytology, DNA ploidy analysis, and
fluorescence in situ hybridization for the detection of dysplasia and adenocarcinoma in patients with
Barrett's esophagus. Hum Pathol 2008;39:1128-1135
Bartonella Antibody Panel, IFA CSF
Reference Values:
REFERENCE RANGE: