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Walia Special Edition on the Bale Mountains (2011) - Zoologische ...

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Observers located each point by GPS, and four subsamples (N/E/S/W) were taken at each<br />

locati<strong>on</strong>. Sampling density was equivalent to that <strong>on</strong> rodent trapping grids, with 5m between<br />

subsamples. For each plant species intersected by <strong>the</strong> sampling rod, observers recorded <strong>the</strong> maximum<br />

height, plant part (leaf, stem or flower) and colour (to indicate seas<strong>on</strong>al changes in nutriti<strong>on</strong>al<br />

quality). Substrate (earth, rock or water), date, time and observer were also recorded.<br />

Vegetati<strong>on</strong> was surveyed by observers without formal botanical expertise, limiting <strong>the</strong><br />

number of taxa which could be identified to species level. Surveys occurred in both wet and dry<br />

seas<strong>on</strong>s, so <strong>the</strong> flower structures necessary for identificati<strong>on</strong> of grasses and some herbaceous species<br />

were not always present. All grass-like plants were <strong>the</strong>refore split into two categories <strong>on</strong>ly: wetland<br />

sedges and grasses. Similarly, no attempt was made to distinguish between different mosses or<br />

lichens.<br />

Ground-truthing and statistical analysis<br />

The land cover type represented by each class was determined using qualitative habitat descripti<strong>on</strong>s,<br />

supplemented by visual examinati<strong>on</strong> of aerial photographs, false-colour composites, 199 groundtruthing<br />

photographs and familiarity with areas gained during field surveys between April 2003 and<br />

April 2005. To assess how related clusters of classes in <strong>the</strong> distance dendrogram differed from <strong>on</strong>e<br />

ano<strong>the</strong>r, pairwise statistical comparis<strong>on</strong>s were made at each node for which sufficient data existed.<br />

Differences in plant species compositi<strong>on</strong> and substrate were assessed using c<strong>on</strong>tingency tables.<br />

General Linear Models (GLMs) were used to test for differences in altitude, slope, aspect, and<br />

percent vegetati<strong>on</strong> cover, and mean sward height was also compared after accounting for species<br />

compositi<strong>on</strong> and altitude. Tests used all classes and all species for which enough data were available;<br />

sample sizes varied depending up<strong>on</strong> which classes were being compared.<br />

The combinati<strong>on</strong> of mountainous terrain, low sun elevati<strong>on</strong> (52.1 degrees) and sun azimuth<br />

(139.6 degrees) at <strong>the</strong> time of satellite image capture caused large differences in illuminati<strong>on</strong><br />

between sou<strong>the</strong>ast and northwest slopes. This meant that classes <strong>on</strong> steeply sloping ground may<br />

have differed from each o<strong>the</strong>r <strong>on</strong>ly in <strong>the</strong> degree of illuminati<strong>on</strong>, ra<strong>the</strong>r than in species compositi<strong>on</strong><br />

or density of vegetati<strong>on</strong>. Therefore we calculated <strong>the</strong> incidence angle of <strong>the</strong> sun’s rays to describe<br />

<strong>the</strong> relative illuminati<strong>on</strong> of each pixel, and test for differences in average illuminati<strong>on</strong> between<br />

classes. The following formula was taken from Dozier and Frew (1990):<br />

θ i = arccos(cosθ 0 *cosS + sinθ 0 *sinS*cos(Φ 0 − A))<br />

where θ = illuminati<strong>on</strong> angle, θ = solar elevati<strong>on</strong>, S = pixel slope, Φ = solar azimuth and A = pixel<br />

i 0 0<br />

aspect.<br />

We investigated seas<strong>on</strong>al changes in photosyn<strong>the</strong>tic activity by categorising plants into ‘alive’<br />

(flowering or green) or ‘dead/dormant’ (brown or yellow). This was hard to determine for some<br />

pubescent species such as Helichrysum sp.; <strong>the</strong> status of <strong>the</strong>se species was assigned as ‘unknown’<br />

unless <strong>the</strong>re was evidence of flowering.<br />

<str<strong>on</strong>g>Walia</str<strong>on</strong>g>-<str<strong>on</strong>g>Special</str<strong>on</strong>g> <str<strong>on</strong>g>Editi<strong>on</strong></str<strong>on</strong>g> <strong>on</strong> <strong>the</strong> <strong>Bale</strong> <strong>Mountains</strong> 101

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