Effects of dietary n-3 polyunsaturated fatty acids and ... - FINS
Effects of dietary n-3 polyunsaturated fatty acids and ... - FINS
Effects of dietary n-3 polyunsaturated fatty acids and ... - FINS
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1 st WorkshopXIII International Feed Technology Symposiumpreparation <strong>and</strong> pelleting calf feed mixture by F. oxysporum <strong>and</strong> F. subglutinans speciesthat were present in one <strong>of</strong> the feed ingredient, because these species are known as weakT-2 toxin producers in Serbia [12].Pelleting did not positively affected on mycotoxicological properties <strong>of</strong> calf feedmixture.From the production day (day 0) until the expiry date (day 90) pelleted as well aspowdered mixture were microbiologically <strong>and</strong> mycotoxicologically correct according toRegulations on maximal quantity <strong>of</strong> harmful materials <strong>and</strong> ingredients in fodder [17].Analysis performed 2 months after expiry date (day 150) showed, with the exception <strong>of</strong>sulphito-reducing Clostridium spp., that total count <strong>of</strong> other microorganisms (bacteria,yeasts <strong>and</strong> molds) as well as contamination with mycotoxin were even reduced (Tables 2- 4).In vitro experiments with two F. verticillioides isolates obtained from powdered calfmixture after 90 days <strong>of</strong> storage showed that this species did not have potential for ZON,T-2 <strong>and</strong> DAS biosynthesis. The similar results i.e. that F. verticillioides can notbiosynthesize ZON <strong>and</strong> type A trichothecenes were presented in our previousinvestigation with different Fusarium cultures isolated from feed ingredients <strong>and</strong>complex animal mixtures [3].CONCLUSIONIt can be concluded that pelleting procedure <strong>of</strong> calf feed mixtures has had the positiveeffect on improvement <strong>of</strong> microbiological proprieties <strong>of</strong> investigated mixtures, but noton mycotoxicological once.In the case <strong>of</strong> adequate storage conditions (moistute, temperature, housekeepingmeasures etc.) calf feed mixture can be used after expiry date because its microbiological<strong>and</strong> mycotoxicological correctnes can be preserved.ACKNOWLEDGEMENTSThis paper was realized within TR 20016 project, financed by Ministry for Science <strong>and</strong>Technological Development <strong>of</strong> Republic <strong>of</strong> Serbia.REFERENCES1. Adamović, M.J., Bočarov-Stančić, A.S., Pantić, V. R., Radivojević, M.A.,Adamović, I.D., Stojanović, B.D.: Influence <strong>of</strong> pelleting on microbiological<strong>and</strong> mycotoxicological correctnes <strong>of</strong> feed mixtures with bentonite supplement,Zbornik Matice srpske za prirodne nauke, 116 (2009), 113-119.2. Bočarov-Stančić, , A., Adamović, M., Miljković, A., Štrbac S., Salma, N :Mikotoksikološka kontaminacija hraniva i krmnih smeša u Banatu, A PVojvodina, Biotehnologija u stočarstvu, 24 (2008), 359-373.3. Bočarov-Stančić, A.S., Lević, J.T., Dimić, G.R., Stanković, S. Ţ-, Salma, N.M. : Investigation <strong>of</strong> toxigenic potential <strong>of</strong> fungal species by the use <strong>of</strong> singlescreening method, Zbornik Matice srpske za prirodne nauke, 116 (2009), 25-32.116
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