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Effects of dietary n-3 polyunsaturated fatty acids and ... - FINS

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1 st WorkshopXIII International Feed Technology Symposium<strong>of</strong> the samples were coocked in the oven at the temperature <strong>of</strong> 80-85 0 C until thetemperature in the centre <strong>of</strong> the meat reached 69 0 C (about 1 hour). After 24 hours, thesamples were sent to the laboratories <strong>of</strong> Food Technology Institute in Novi Sad, where<strong>fatty</strong> acid analysis <strong>and</strong> sensory evaluation were performed.Fatty acid analysisThe preselected meat samples were homogenized with food processor <strong>and</strong> fat wasextracted from 1 g <strong>of</strong> each sample. From the extracted lipids <strong>fatty</strong> acid methyl esterswere prepared with boron trifluoride/methanol solution. Obtained samples were analyzedby a gas chromatograph Agilent 7890A system with FID, auto-injection module forliquid <strong>and</strong> headspace sampling, equipped with fused silica capillary column (DB-WAX30 m, 0.25mm, 0.50 um). The <strong>fatty</strong> <strong>acids</strong> were identified by comparison with st<strong>and</strong>ardsfrom Supelco 37 component FAME mix <strong>and</strong> data from PUFA NO.2, Animal sourceBCR-163 beef/pork fat blend. Results were expressed as mg <strong>of</strong> <strong>fatty</strong> <strong>acids</strong> per 100 g <strong>of</strong>tissue (mg/100g) <strong>and</strong> as a ratio between omega-6 <strong>and</strong> omega-3 <strong>fatty</strong> <strong>acids</strong>.RESULTS AND DISCUSSIONDuring the study the animals were readily fed <strong>and</strong> there was no illnesses or losses. Allpiglets were similar in size, healthy, with shiney <strong>and</strong> clean hair <strong>and</strong> vivacious. Results <strong>of</strong>monitoring the avarage weight, average weight gain, feed consumption <strong>and</strong> feedconversion ratio are shown in the Table 2.Table 2. Average weight <strong>and</strong> weight gain <strong>of</strong> piglets, feed consumption <strong>and</strong> feedconversion ratioAverage weight, kg035.day79.dayAverage weightgain, kg/day1-35.day35-79.dayFeedconsumption,kgFeedconversionratio, kg/kgControl 1,70 9,00 30,00 0,209 0,477 45,00 2,14Experimental 1,70 10,50 35,00 0,251 0,557 40,00 1,63From the results shown in Table 2, it is evident that piglets from the treatment group fedwith diet enriched with extruded linseed (Vitalan) consumed 40 kg <strong>of</strong> feed while thepiglets from the control group fed with the diet without enrichemnet consumed 45 kg <strong>of</strong>feed. Even though they consumed less feed, the piglets from the treatmnet group, with201

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