Effects of dietary n-3 polyunsaturated fatty acids and ... - FINS

1 st WorkshopXIII International Feed Technology SymposiumPreparation of starch hydrolyzates from corn meal and triticale [8,9]Starch hydrolyzates were obtained by a two step hydrolysis (liquefaction andsaccharification). A 100 g of corn meal was mixed with water at the weight ratio 1:3, and60 ppm of Ca 2+ (as CaCl 2 ) ions was added. The liquefaction was carried out at 85ºC andpH=6.0 for 1h by adding 0.026% (v/w starch) enzyme Termamyl SC. The liquefiedmash was saccharified at 55ºC and pH=5.0 for 4h with 0.156% (v/w starch) enzymeSAN Extra L. The hydrolysis was performed in flasks in a thermostated water bath withshaking at 150 rpm.Grinded and mashed triticale samples were mixed with water warmed at 50 C in metallicjars, keeping the sample to water ratio at 1:3. After mixing of the samples with waterTerm amyl 120 L was added. The samples were held in a water bath with mixing(150 rpm) for 30 min at 50 C. After that triticale samples were heated to 60 C. At thistemperature the samples were held for 60 min with mixing. Temperature was thenlowered to 55 C and SAN Super 240 L was added. Samples were held on thistemperature for 30 min after which the temperature was lowered to 30 C.Laboratory preparation of cell culture [8]Saccharomyces cerevisiae was used for the fermentation of the hydrolyzed corn mealand triticale samples. The culture originated from the collection of BIB-TMF, Belgrade,and was maintained on a malt agar slant. Before use as an inoculum for the fermentation,the culture was aerobically propagated in 500 ml flasks in a shaking bath at 30ºC for 24h and then separated by centrifugation.Ethanol fermentation of starch hydrolyzatesStarch hydrolyzates were subjected to ethanol fermentation by Saccharomycescerevisiae under anaerobic conditions (pH=5.0; 30ºC). The initial yeast concentrationwas 2%. Fermentation time was 36 hours.Separation of stillage after distillationAfter ethanol distillation, the stillages of corn and triticale were removed bycentrifugation. The solid samples were dried at 60 0 C and used for chemical analysis.Enrichment of corn stillage with yeastDried corn stillage was enriched with spray dried Saccharomyces cerevisiae (1%w/wstillage) [10].RESULTS AND DISCUSSIONThe results of the chemical composition of corn stillage, the stillage of triticale and thecorn stillage enriched with yeast are presented in Table 1.301