Effects of dietary n-3 polyunsaturated fatty acids and ... - FINS

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1 st WorkshopXIII International Feed Technology Symposium6. Gliddon M. J., Wright D., Markantonatos A., Groth W.: Determination ofmaduramicin amonium in poultry feedstuffs by high-performance liquidchromatography, Analyst, 113(5) (1988), 813-816.7. Glišović L., Agbaba D.: Denzitometric determination of salinomycin in animalfeeds. Acta Veterinaria, 42, 5-6, (1992), 325-328.8. Dimenna G.P., Lyon F.S., Thompson F.M.: Effect of antibiotic combination,dosing period, dose vehicle and assay method on Salinomycin residue levelsand their ionophoricity in chicken tissues, J. Agric. Food Chem, 37 (1989),668-676.9. Elliott C.T., Kennedy D.G., McCaughey W.J.: Methods for the detection ofpolyether ionophore residues in poultry. Analyst, 123 ( 1998 ), 45-56.10. Jenkins M., Allen P., Wilkins G., Klopp S., Miska K.: Eimeria praecoxinfection ameliorates effects of Eimeria maxima infection in chickens. Vet.Parasitol. 155, 1-2 (2008), 10-4.11. Kostadinović Lj.: Effects of coccidiostatics on biochemistry parameters ofbroilers and determination their residues in tissues. Thesis. University of NoviSad, 1998.12. Kostadinović Lj., Pavkov S.: A quantitative and screening method fordetermination of salinomycin in drugs and feed, Proc. I Congress of pharmacyof the Republic of Macedonia, 1995, 1-2, 266-268.13. Hagel R.B.: Liquid chromatographic determination of lasalocid in premixes, J.Assoc Off Anal Chem, 61, 5, (1978), 1070-1073.14. Heitzman R.J.: Veterinary drug residues, Second Edition, Commission of theEuropean Communities, Sg.4.4./1: Ionophores – A Routhine method for thedetermination of residues of ionophores in chicken meat, liver and kidney,using HPTLC, United Kingdom, 1996.15. Campbell H.: HPLC analysis of monensin, narasin and salinomycin in feeds,using postcolumn derivatization, Antibiotic and drug workshop, 103 rd Annualinternational meeting of the AOAC, St. Louis, USA, 1989.16. Constantinoui C.C., Molloy J.B., Jorgensen W.K., Coleman G.T.:Development and validation of an ELISA for detecting antibodies to Eimeriatenella in chickens, Veterinary Parasitology, 150, 4, (2007), 306-313.281
1 st WorkshopXIII International Feed Technology Symposium282QUALITY OF FEED INGREDIENTS – CHEMICAL,MICROBIOLOGICAL AND MYCOTOXICOLOGICALPARAMETERSAleksandra Miljković, Nataša Salma, Aleksandra Bočarov-Stančić, Vladimir Pantić,Bisera Dolić, Danijela Mihaljčić"Center for Bio-Ecology"d.o.o., Zrenjanin, Petra Drapšina 15, 23000 Zrenjanin, SerbiaABSTRACTChemical, microbiological and mycotoxicological analysis included 11 different types ofcomponents of feed mixtures, 62 samples respectively, that were analyzed in theaccredited laboratory in "Bio-Ecological Center" Ltd. in Zrenjanin from the beginning of2009. Content of proteins was not adequate in 4.1% of tested samples, as well ascellulose content. Higher fat content was found in 6.1 % of feed ingredients, and higherprotease activity in 2.0% of the samples. The most of samples (82.6%) matched theSerbian normatives for feed. The maximum number of bacteria (6.3 x 10 6 /g), as well asyeasts and molds (9,267/g) was detected ino wheat grains harvested in 2009.Microbiological not adequate quality of the 4 analyzed samples was a consequence ofthe presence of Proteus species. Mycological analysis established the presence of 18types of molds, and 10 genera respectively. The dominance of representatives of thegenus Fusarium (56.52%) was reported with F. subglutinans and F. verticillioides as themost widespread species. Despite the findings of a number of potentially toxigenicmolds mycotoxicological analysis in most cases gave negative results. From six analyzedmycotoxins (aflatoxin B1, ochratoxin A, zearalenone - ZON, deoxinivalenol, T-2 toxinand diacetoxyscirpenol) only two were identified. ZON, in the amount of 0.294 mg/kgwas contaminant of one sample of wheat grains, and in the amount of 1.472 mg/kg onesample of crushed oil sunflower seed. Trichotecen of type A - T-2 toxin was determinedonly in herd pea in the concentration of 0.500 mg / kg.Key words: feed ingredients, chemical, microbiological and mycotoxicologicalparametersINTRODUCTIONIt is well known that one of the priorities of every nutritionist is the improvement of theproduction potential of domestic animals as well as getting the product of high-quality.Balanced diet that matches the needs of certain categories of animals provides a goodhealth and good reproductive state and thus reaching the desired production goals [6].Quality of animal feed does not only means the optimal nutrient value and health safety(microbiological quality) but also the absence of harmful substance. Components of feedmixtures may be contaminated with toxigenic molds and their toxic metabolites(mycotoxins) that can cause health disturbances of animals, and sometimes even theirdeath. Seriousness of their impact depends not only on the amount of toxic substancethat enters the body, but also upon the frequency of the uptake. Small dose of
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