Effects of dietary n-3 polyunsaturated fatty acids and ... - FINS

1 st WorkshopXIII International Feed Technology SymposiumMATERIAL AND METHODSExperiment was organized as two-factorial (3 3), with three replications, where factorA was plant material and factor B type of additive (Table 1). Maize hybrid ZP-677 wasensiled in the wax phase of grain development, while lucerne of the NS Mediana ZMS 5cultivar was used at the beginning of bloom and after short wilting. Urea that was usedwas chemically pure with the crude protein equivalent of 291.6%. Thehomofermentative inoculant that was used had according to the producermicrocapsulated bacteria Lactobacillus plantarum (min. 1,0 10 11 CFU), Lactobacillusacidophilus (min. 1,0 10 11 CFU), Streptococcus faecium (min. 1,0 10 11 CFU) andPediococcus acidilactici (min. 1,0 10 11 CFU). The amount of inoculant was usedaccording to the recommendations, 250 g/10 t green mass of maize and 250 g/5 t forlucerne. Organozeolite was product of Institute for Technology of Nuclear and OtherRaw Materials (Belgrade, Serbia).All experimental silages after the treatment with the additives were compressed in plasticsiloses with volume of 60 dm 3 . After 56 days the siloses were opened and representativesamples were collected for analysis. Parameters of chemical composition and quality ofsilages [1] were analyzed in the Laboratory for animal nutrition at the Faculty ofagriculture, Zemun-Belgrade. Statistical analysis was done by analysis of variance withPC software StatSoft v. 6 [18].Table 1. Additives used in experimentMaterialAdditiveWhole maize MaizeLucerneplant cobMaize meal, 50 g/kg + - -Inoculant, 250 g/10 t green mass of maizeand 250 g/5 t for lucerne+ - +Urea, 5 g/kg - + -Organozeolite, 2 g/kg - + +RESULTS AND DISCUSSIONCompared with the starting material all silages had somewhat more dry matter (Table 2).That was contribution of the additives, but also partly because some volatile substanceswere lost during the drying of samples. Silages were dried at 80°C and than the drymatter was corrected for the amount of volatile substances (acetic and butyric acid,ammonia and alcohol). The amount of such materials was very small [5].Amount of crude protein in silages was lower compared with the starting material whichis the result of some nitrogen loses in form of ammonia. The exception was whole plantmaize silage with urea, where protein content was increased. Crude protein analyzed byKjeldahl process reflects only the nitrogen contents, not the true protein [7].Variability of lipid content is explained with the extraction of the part of the lactic acidwith diethyl-ether [2], so that the highest amount of ether extract was detected in the147