Effects of dietary n-3 polyunsaturated fatty acids and ... - FINS

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1 st WorkshopXIII International Feed Technology SymposiumTable 3. Differences between repeated measurements for the gravimetric and TGAmethodMeanMeasurement 1 Measurement 2 t p SELGravimetric method8.10 8.13 0.13 0.894 0.22TGA method9.34 9.33 0.04 0.969 0.05CONCLUSIONComparison of the results of the gravimetric and TGA methods used for the determination ofcrude ash content in feedstuffs showed that the results for the TGA method had demonstratedbetter precision compared with the reference chemical method. Furthermore, the results ofthis study showed the existence of a certain bias between the results of two methods, wherethe gravimetric method produced results that were lower than the results obtained by thegravimetric method. The differences in obtained results may be affected by differentphysical nature of the methods, different equipment used, different measurementconditions, uncertainties of mass equipment and temperature controllers, computationeffects, operator effects or random variation. Further work should be performed in orderto identify the level of accuracy of these methods.ACKNOWLEDGEMENTSThis work was financed by the Ministry of Science and Technological Development,Republic of Serbia (Project No. 20066).REFERENCES1. Danzer, K: Analytical chemistry: theoretical and metrological fundamentals.Springer-Verlag Berlin, Heidelberg 2007, pp 65-100.2. Eurachem/CITAC Guide CG 4: Quantifying Uncertainty in AnalyticalMeasurement, Eurachem/CITAC Working Group, Eurochem, London (2000).3. Felsner, M.L., Cano, C.B., Matos, J.R., de Almeida-Muradian, L.B., Bruns,R.E: Optimization of thermogravimetric analysis of ash content in honey. JBraz Chem Soc, 15 (2004), 797-802.4. Hoffman, P. C: Ash content of forages, Focus on Forage, 7 (2005), 1-2.5. Hunt, L.A: Ash and energy content of material from seven forage grasses,Crop Sci, 6 (1966), 507-509.6. Jovanović, R., Dujić, D., Glamočić, D: Ishrana domaćih životinja. Stylos,Novi Sad, 2002.197
1 st WorkshopXIII International Feed Technology Symposium7. Pérez-Marín, D.C., Garrido-Varo, A., Guerrero-Ginel, J.E., Gómez-Cabrera, A: Near-infrared reflectance spectroscopy (NIRS) for the mandatorylabelling of compound feedingstuffs: chemical composition and opendeclaration,Anim Feed Sci Technol, 116 (2004), 333–349.8. Pojić, M., Mastilović, J., Pestorić, M., Daković, S: A comparative study oftwo analytical methods for fat content determination in brewer’s grits, J AmSoc Brew Chem, 67(2009), 166-169.9. Schenz, T.W., Israel, B., Rosolen, M.A: Thermal analysis of water-containgsystems, in: Water relationships in foods: advances in the 1980s and trends forthe 1990s. Eds. H. Levine and L. Slade. Plenum Press. New York 1991, pp199-214.10. Tomassetti, M., Campanella, L., Aureli, T: Thermogravimetric analysis ofsome spices and commercial food products: Comparison with other analyticalmethods for moisture content determination (part 3). Thermochim Acta, 143(1989), 15-26.11. Van Soest, P. J: Nutritional Ecology of the Ruminant. Cornell UniversityPress, Ithaca 1994.12. Williams, P. C: Sampling, sample preparation, and sample selection, in:Handbook of Near-Infrared Analysis. Eds. D. A. Burns and E. W. Ciurczak,CRC Press, Boca Raton, FL. Pp. 267-295, 2008.)13. Windham, W.R., Hill, N.S., Stuedemann, J.A: Ash in forage, esophageal,and fecal samples analyzed using near-infrared reflectance spectroscopy. CropSci, 31 (1991), 1345-1349.198
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