Effects of dietary n-3 polyunsaturated fatty acids and ... - FINS

1 st WorkshopXIII International Feed Technology SymposiumRegulations on changes and supplements of regulations on the quality and otherrequirements of fodder [17]. Other parameters given in Table 2 were analyzed at therequest of users, in order to obtain information necessary for the preparation of feedmixtures for different categories of domestic animals according to the nutritiousrecommendations given by Sinovec and Ševković [12].Table 3. Microbiological quality of fodder ingredients analyzed in 2009SampletypeNo. ofsamplesBCFU/g(aver. value)MYCFU/g(aver. value)Adequatequality (%)Wheat grain 3 6303000 9267 100Wheat brain 1 1070000 920 100Barley grain 4 4312500 3530 100Triticale 1 2350000 3400 100Herd pea 1 655000 330 100Protilac 1 1700 150 100Soybean cake 4 278000 345 75Soybean grits 1 720000 300 0Sunflower pellet 5 3386200 5790 60Flax seed 1 148000 1600 100Corn silage1 565000 1900 100TOTAL 23 1700 – 6303000 150 – 9267 82.6Legend: BCFU/g – bacterial colony forming units per gram,MYCFU/g – colony forming units of molds and yeasts per gramThe majority of tested samples (82.6%) had satisfactory microbiological qualityaccording to the Regulations on maximal quantity of harmful materials and ingredientsin fodder [15]. The maximum numbers of bacteria (6.3 x 10 6 /g), as well as yeasts andmolds (9,267/g) were detected in wheat grain samples harvested in 2009, while thelowest number of microorganisms (1700 BCFU/g and 150 MYCFU/g) was recorded inProtilac additive.Microbiologicaly anadequate quality of 1 sample of soybean cake, and 2 samples ofsunflower pellets was a consequence of the presence of Proteus species. From otherpathogenic bacteria only Clostridium spp. were found in one sample of soybean cake,but in much smaller number compared to the max allowed number according to Serbianregulations (20/g compared to 1000/g).286