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Effects of dietary n-3 polyunsaturated fatty acids and ... - FINS

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1 st WorkshopXIII International Feed Technology SymposiumThere was significant variation <strong>of</strong> NFE in lucerne silages was result <strong>of</strong> the carbohydrateadditives used. Maize plant silage without additive had the lowest amount <strong>of</strong> NFE,which is result <strong>of</strong> the intensive fermentation <strong>and</strong> the use <strong>of</strong> fermentable carbohydratesfor organic acid synthesis. For that reason all silages had lower NFE compared with thestarting material.Significant variations <strong>of</strong> mineral matters were observed only in lucerne silages because<strong>of</strong> carbohydrate additives.The highest pH values were observed in lucerne silages (Table 3), which was expectedconcerning the high buffering value <strong>of</strong> that plant <strong>and</strong> its previous wilting [4]. Whencarbohydrate additive <strong>and</strong> inoculant were used there was significant decrease in pHvalue in lucerne silage. Adding urea to the whole plant maize silage resulted insignificant increase <strong>of</strong> pH values. In spite <strong>of</strong> that, such silages had pH values within theoptimum range [7].The use <strong>of</strong> inoculants in maize cob silages resulted in furtherdecreases <strong>of</strong> pH values, below the optimum range.Table 3. Parameters <strong>of</strong> biochemical changes in silagesTreatments pHAcids, g/kg DMNH 3 -N,AceticButyricg/kg N LacticF B T F B TLucerne 5.02 a 156.17 a 41.45 b 9.53 b 42.58 a 52.11 ns - - -Lucerne + 50g/kg maize 4.96 b 106.84 b 45.17 b 11.25 b 38.30b a 49.55 ns - - -mealLucerne +inoculant4.78 c 107.52 b 58.72 a 16.32 a 32.18 b 48.50 ns - - -Whole maizeplant3.83 b 69.73 b 52.38 a 15.56 a 7.18 ns 22.74 ns - - -Whole maizeplant + 5 4.12 a 332. 25 a 36.04 c 11.87 b 8.25 ns 20.12 ns - 3.88 3.88g/kg ureaWhole maizeplant + 2 3.85 b 24.41 c 41.28 b 10.13 b 8.34 ns 18.47 ns - - -g/kg zeoliteMaize cob 3.88 a 56 a 28 b 7 a 3 b 10 ns - - -Maize cob +inoculant3.63 b 42 ab 37 a 6 a 5 a 11 ns - - -Maize cob +organozeolite3.96 a 47 b 30 b 3 b 6 a 9ns - - -Statistical significance (P)A ** ** ** * * * - * *B ** ** ** * * ns - * *a,b,c Values in same column for same plant material with different letter is significantlydifferent (P 0.05) ns = no significance. S – free, B – bonded, T – total.The pH value <strong>and</strong> dry matter content are most important factors that dictate theproteolysis intensity, but they cannot completely stop it [3]. The amount <strong>of</strong> ammonianitrogen in relation to total nitrogen is the first <strong>and</strong> fundamental indicator <strong>of</strong> the degree149

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