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using EPFC. The intradermal tuberculin comparative test was applied to all animals at 12 and 24 months postvaccination. IFN production of cell cultures stimulated with bovine PPD showed significant increase (1.4, 0.90, 1.4 OD) at days 15, 90 and 180 postvaccination, in the group innoculated with EFPC. In the group vaccinated with BCG the increase was observed at 60 and 150 days postvaccination (0.90, 1.7 OD) while the control group showed increment at day 90 (1.35 OD). Animals from the three groups showed an increse (1.4 OD) at day 60 when stimulated with avian PPD. Stimulation with ESAT 6 did not give a clear response in any of the groups. With respect to the humoral response, all groups showed an increase in OD at day 90 but was significantly higher (P
esponse in the eye produces accumulation of polymorphonuclear neutrophils (PMNs) in tissue and ocular fluids, increase of the vascular permeability and therefore an increase of AH proteins. The increase of PMNs explains the larger concentrations of TMS reached in AH of the animals with keratoconjunctivitis. Although TMS levels in AH (either at 5 or 10 mg/kg) persisted during 72-96 h, they never surpassed the M. bovis MIC (< 1 µg/mL). Nevertheless a longer TMS persistence was evident in sick animals, being documented through longer mean residence time (41.2, 45.7 and 22.7 vs 15.9 h for the G1, 2, 3 and 4 respectively) and a smaller clearance from AH (222.8, 469.5, 773.3 vs 1613.5 mL/h/kg for the G 1, 2, 3 and 4, respectively). These information suggest that TMS penetrate to the ocular balloon and concentrates in AH depending on the degree of inflammation. Concentrations in vitreous humors and ocular tissue, should be evaluated in the future to consider the use of the TMS for the treatment of certain ocular infections. Key words: tilmicosin, aqueous humor, keratoconjunctivitis, pharmacokinetic, cattle 697 The use of PCR for Identification of Brucella abortus in Samples from Aborted Fetuses in a Dairy Farm in the State of Aguascalientes, Mexico D. Limón 1 , G. Martínez 1 , G. Zenteno 1 , B. Arellano 2 , E. Diaz 1 , R. Flores 1 , F. Morales 1 1 INIFAP, CENID Microbiologia, MEXICO, D.F., Mexico 2 Universidad Nacional Autonoma de México, Facultad De Medicina Veterinaria, MEXICO, D.F., Mexico Abomasal fluid and tissues conserved in 10 % bufferated formalin solution from 13 aborted fetuses, as well as sera from 55 aborted cattle were collected in a dairy farm located in Aguascalientes, Mexico and were transported to the Diagnostic Laboratory of CENID- Microbiologia, INIFAP. The abomasal fluid samples were inoculated in enriched Farrel’s medium in order to attempt the Brucella abortus isolation. The tissues samples were processed for histophatological analysis, while the sera were used for detection of BVD, IBR, Neospora, Leptospira and Brucella antibodies. Those Brucella spp suggested isolations were examined by using a PCR procedure. Depending of the amplified fragments (361, 456, 1063 and 1298 bp) it is posible the identification and differentiation of field and vaccinal Brucella abortus strains. Six isolated strains were cultured from the abomasal fluid and all of them were identificated as Brucella abortus field strains by PCR. Brucellosis typical lesions (multifocal necrotical hepatitis and exudative pneumonia) were observed in 4/13 studied fetuses. The result of serological examination of 55 samples was as follows: Brucellosis, 27 %; Neosporosis, 40 %; Leptospirosis, 65 % (titers > 1:400) and IBR, 100% and BVD 80 %. During the last few years Leptospirosis and Neosporosis have shown an increase in their importance as a cause of abortion in cattle in Mexico, our results in the present study confirm this affirmation. The fact that a specific vaccination program to prevent neosporosis does not exist in the dairy farm under investigation, makes it important to consider this agent as important causal of the abortion. In regards to Brucellosis, it is a disease with high prevalence in the dairy cattle farms despite the fact that there is an intensive official control campaign in Mexico and excellent vaccines such as S19 and SRB51 exist; for this reason it is important to have diagnosis techniques with high sensivity and specificity as it is the case of PCR technique. It is important to promote the use of integral etiological diagnosis of abortions in order to be in conditions to apply adequate and effective control measurements. It often occurs that in some laboratories in Mexico the samples are tested only for diagnosis of brucellosis and do not consider the use of techniques for determinate the possible presence of other microorganisms associated with reproductive problems. Financial support SAGARPA-CONACyT. (2004/COI/23) Key words: PCR, Brucella abortus, aborted fetuses 698 Long-Term Excretion of Brucella abortus, Strain B19 in Milk and Urine by Vaccinated Adult Female Bovines M. Genovez 1 , W. Pacheco 1 , C. Pozzi 2 , L. Paulin 1 , M. Gamberini 3 , S. Azevedo 4 1 Instituto Biológico, Animal Health Center, Sâo Paulo, Brazil 2 Instituto de Zootecnia, Animal Health Center, Nova Odessa, Brazil 3 Universidade Federal de Goiás, Animal production, Goiania, Brazil 4 Universidade Federal De Campina Grande, Department for Infectious diseases- Epidemiology, Patos, Brazil Abortion caused by Brucella abortus is a common bovine reproductive disease in many countries and may be currently prevented by vaccination with live B19 vaccine, administered to 3-8 month old heifers. The length of excretion, its relation to the hormonal cycle and its effect on contactant and susceptible animals, including man, are not completely known. The persistence of B19 excretion in milk and urine by adult dairy cows vaccinated at 3-8 months old was investigated in this study. Samples were collected during 12 months (15 collections), beginning at estrus until immediate post-partum, from 14 females in three groups: G1(3-4 years old), G2 (5-6 years old) and G3 (7-9 years old). Milk and urine samples were monitored by bacteriologic analyses and Multiplex PCR differential for B.abortus and B19 strain; blood serum samples were analyzed by Rose Bengal test for brucellosis diagnosis and direct estradiol ELISA KIT and progesterone ELISA KIT (Diagnostics Biochem., Canada) were used for hormone monitoring. All milk samples were negative in serology and bacteriologic examination. B19 was detected by PCR in 74/210 (35.2%) and 12/210 (5.7%) from urine and milk samples, respectively (p=0.001). Excretion mainly occurred from the beginning of estrus to 150 days of pregnancy and for 30 days after parturition. Hence, excretion was intermittent, no matter the age of the animal and the time after vaccination. Hence, excretion was intermittent, no matter the age of the animal, the time after vaccination, and occurred in cows up to nine-year old. Hormonal stage did not seem to affect B19 excretion, and may be associated with erythritol scarcity. The impact of longterm B19 excretion requires more attention. B19 may remain in the environment with a real possibility of transmission to negative cattle herds, mainly to bulls, or even to other susceptible animal species, including man. Urine was the best sample for B19 detection. This paper was supported by FAPESP: Fundaçao de Amparo a Pesquisa do Estado de Sao Paulo, Brazil Key words: bovine brucellosis, B19 excretion, hormonal cycle 699 Main Causes of Abortion in Cattle at Different Areas of Mexico G. Zenteno 1 , F. Manrriquez 1 , I. Vitela 2 , L. Diaz 4 , R. Flores 1 , D. Martinez 3 , F. Morales 1 1 INIFAP, CENID Microbiologia, México DF, Mexico 2 Instituto Tecnológico el Llano, Microbiología, Aguascalientes, Mexico 3 Universidad Veracruzana, Facultad de Medicina Veterinaria y Zootecnia, Facultad De Medicina Veterinaria, Veracruz, Mexico 4 Gobierno del Estado de Aguascalentes, Comité de Fomento y Protección Pecuaria, Aguascalientes, Mexico Data related to the incidence and prevalence of etiological agents associated with reproductive failures in cattle of Mexico are the result of isolated studies, only a few cases include results of integrated diagnosis. Some papers describe the results of serological profiles, but in most of them the interpretation of the results does not permit the diagnosis of the etiological agent. For example, previous studies performed by our research group demonstrated different prevalences of brucellosis, BVD, IBR, neosporosis and leptospirosis as follows: The brucellosis prevalence was always high (12-21 %) except in Veracruz (3%); Leptospirosis was similar to brucella with the highest prevalence in Aguascalientes (27%) and the lowest in Veracruz (3%). The BVD and IBR highest prevalences were observed in the same states (56-86%) while in Veracruz there were lower (47 and 38 % respectively). The prevalence of neosporosis varied between 30 and 45 % in most states, but in Veracruz was only 15 %. With these results it was impossible to relate the abortions with a particular infectious agent. The purpose of this study was to perform an integral, comparative serological profile, in sera collected from aborted and non aborted animals from 7 dairy cattle in different areas of the state of Aguascalientes, Mexico. Blood samples were collected from cattle aborted during a five month period previous to the collection and from non aborted animals. All samples were tested for the identification of Brucelosis, BVD, IBR, Neosporosis and Leptospirosis antibody titers. The results of this serological study, were then used in order to compare the prevalences of each etiological agent with either, aborted and non aborted populations. The results of this comparative study demonstrated that in some farms more than one etiological agent was found to be important: in 4 cattle farms brucellosis was a serious problem; in one farm the main cause of abortion was IBR, while BVD and neospora appeared to be the most important causes of abortion in Infectious and Zoonotic Deseases (Public Health) 101
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130. évfolyam Különszám II. Vol
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The World Buiatrics Congress (WBC)
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lood and liver tissue. Absolute vit
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RNS causes increased lipid peroxida
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An experiment was conducted to stud
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2 Hungarian Institute of Agricultur
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and plasma HCO 3 - increased from H
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Materials and Methods: Serum sample
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vitamin A stores in liver, the hypo
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andomly assigned to one of five tre
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observed in group II of the animals
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The aim of the monitoring was to co
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health group (HG) categories for an
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lactating cows is sparse. Lotan and
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491 Effect of Acarbose on Milk Yiel
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499 Secondary Hepatogenous Photosen
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they all died in a two to three day
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and HCO 3 level. The concentration
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amount of saponins was observed in
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Water was offered ad-libitum. Feed
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Se, I and Co) more than 500 blood s
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541 Variability of Blood Profile an
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evaluated, highly significantly gre
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we measured sensitivity of the enti
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were 0.17; 0.13 °C (Max.: 0.73). D
Page 52 and 53: calving pregnancy period were the b
Page 54 and 55: 2 University of Nottingham, Departm
Page 56 and 57: animals) was treated with 5 mg enro
Page 58 and 59: In dairying, good management practi
Page 60 and 61: Somatic cell count (SCC) is one of
Page 62 and 63: This investigation was run in some
Page 64 and 65: eproductive data showed that cows f
Page 66 and 67: (3.6%) and others (Bacillus spp., N
Page 68 and 69: 602 Coagulase Gene Polymorphism of
Page 70 and 71: 2 Institute of Veterinary Bacteriol
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Page 76 and 77: incubated for one hour at 37 oC. Af
Page 78 and 79: In Belgium, an increasing amount of
Page 80 and 81: Conclusion: It can be concluded tha
Page 82 and 83: calves recovered after 4 to 10 days
Page 84 and 85: disease. Thus losses were estimated
Page 86 and 87: 635 Prevalence of the Pulmonary Les
Page 88 and 89: Conclusions The sanitation program
Page 90 and 91: Italy. After the sample examination
Page 92 and 93: Results: The cows sera were collect
Page 94 and 95: (Mb) as co-infecting agent is becom
Page 96 and 97: distributed over 13 different fatte
Page 98 and 99: main infection sources to the urban
Page 100 and 101: 686 Characterisation of Cattle Orig
Page 104 and 105: three cattle farms each. Leptospiro
Page 106 and 107: (cELISA) only to definitively confi
Page 108 and 109: 1 Islamic Azad University of Shahre
Page 110 and 111: Financial support: CNPq, CAPES, FIN
Page 112 and 113: 731 Detection and Investigation on
Page 114 and 115: necropsied, tissue samples were tak
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Page 120 and 121: decrease in animal aggregation in p
Page 122 and 123: 747 Effect of Dietary Supplementati
Page 124 and 125: 755 Study of Pulmonary Adenomatosis
Page 126 and 127: 1 University of Erciyes, Faculty of
Page 128 and 129: 2006 was observed a small decreasin
Page 130 and 131: for O69 strain), and clinical signs
Page 132 and 133: 2 University of Veterinary Medicine
Page 134 and 135: 794 Serum Haptoglobin and Fibrinoge
Page 136 and 137: complete random design (CRD). The r
Page 138 and 139: - Ca, Mg, K, and Ca:P ratio were lo
Page 140 and 141: 818 Cerebral Coenurosis in a Goat:
Page 142 and 143: concentrations of the goat milk, in
Page 144 and 145: was significant and showed that bre
Page 146 and 147: cm long and 1/48 cm diameter. Middl
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Page 150 and 151: 847 Llama Anesthesia for Orthopedic
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28 Preliminary Results of a General
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Israeli commercial dairy herds cons
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109 Association between Johne’s D
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195 Changes in Plasma Beta-Carotene
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In the last year 2006, in the perio
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serious economic concern in the fat
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was a cumulative incidence rate of
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The level of antibodies produced in
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1096 Determination of Risk Indicato
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ParaCheck, CSL/Biocor, Australia (C
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1111 The Prevalence of Hock Lesions
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Objectives: To evaluate two differe
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1126 Relationships between Paratube
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1134 A Comparison between Two Calf
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The present work aimed to investiga
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University College Dublin, School o
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214 Cystic Ovarian Diseases in Dair
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inseminator were tested in regressi
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University of Nottingham, Animal He
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tissue (8,6 cm 2 ) was twice as lar
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libido in unmotivated males. Simila
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groups according to their diameter
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improve embryonic development are m
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in each sample). On day 0, 104 cows
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1 Warsaw University of Life Science
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and durable spermatozoa. Within the
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motility and CM integrity (p=0.869
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the median number of attempted moun
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909 The effect of “GnRH” and
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3 COOPEVOLIA, Centre Zootechnique,
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923 Production of Bovine Blackleg V
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Institute of Physiology and Sanocre
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2 University College Dublin, Animal
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(I.M.) 1 mg of estradiol cipionate
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Lameness results in major economic
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methods; ANOVA (with binary classif
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Initially two radiographs were obta
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number of positive faecal samples d
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Pfizer Animal Health, Veterinary Me
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Key words: calf, active immunity, p
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1 University of Camerino, Veterinar
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vaccinated (Group II) with Pastobov
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# Antigens Association in diarrhea
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1015 Prevalence of Eimeria Infectio
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group showed higher serum lactate c
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thymus in calves and heifers with c
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concentrations of calves with umbil
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The goal of this paper is to expose
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187 Cooling Strategies to Improve M
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inspections, carried out by specifi
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The origin of Hungarian Grey cattle
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University of Warmia and Mazury in
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POSTER ABSTRACTS 1225 The Effect of
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multiple fracture (n=1, 1.3%). The
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position. A fusiform skin incision
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Conclusions: It is the first presen
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1 Faculty of Veterinary Medicine, A
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of 101.55±4.94 mmHg. Afterwards th
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In Mexico, there is a wide variety
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Cardiovascular disorders are common
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Conclusion: The total contamination
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Material and methods: The trial inc
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detection of M. wenyonii infection.
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390 Study on Attenuation Mechanisms
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POSTER ABSTRACTS 1152 Detection of
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digestion. It works only on Apicomp
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determined. In addition the surface
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Africa and Europe, which causes mor
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Results: On D0, the animals include
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addition of mineral salt, during 12
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digestion could be improved with im
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Ltd., Blandford Forum, UK). The air
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cattle ocular squamous cell carcino
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pharmacokinetic/pharmacodynamic (PK
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Bovine Immunodeficiency Virus (BIV)
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significantly lower in groups vacci
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5.00+0.67 and 4.94+0.15 mEq/l in th
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potable water and food, avoiding st
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equal distributions at 08:00 and 16
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electrochemical properties of the a
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Selenium was discovered as an essen
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growth by measuring BW at 15-day in
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influence of different risk factors
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performances of primiparous cows ar
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anaerobes. Aerobic bacteria isolate
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P4 12 to 24 hours before calving is
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exists about Mb prevalence in dairy
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frequency of animals with BoHV anti
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esults, supported by a decrease in
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international drivers of change. Th
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The performance of two groups of no
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spasm is accompanied by kyphosis, w
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occurrence of a crisis of stiffness
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Notices 336 XXV. Jubilee World Buia
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