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Oral and Poster Abstracts

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cm long <strong>and</strong> 1/48 cm diameter. Middle cardiatic vein goes towards<br />

coronary sinus <strong>and</strong> it drains into it. This vein <strong>and</strong> interventricular<br />

subsinousal artery together lie on right longitudinal groove. Another large<br />

vein is observed in buffaloes’ heart. It is called ventricular margine vein.<br />

Results of this survey are established that buffaloes’ heart is different from<br />

other ruminants’ heart. Ruminants’ heart is monocoronary type but<br />

buffaloes’ heart is bicoronary.<br />

Key words: anatomy, buffalo, heart, vein<br />

842 Plasma Concentrations of Testosterone <strong>and</strong> Androsteneioda<br />

in Male Buffaloes (Bubalus Bubalis) Originated from<br />

Tropical Regions<br />

J. Siqueira, E. Oba, R. Bittencourt, L. Leal<br />

FMVZ-UNESP, 1Department of Animal Reproduction <strong>and</strong> Veterinary<br />

Radiology, Botucatu-SP, Brazil<br />

Despite of the important functions that the hormones play in physiology,<br />

it is observed that few studies about reproductive endocrinology are<br />

related to the male <strong>and</strong> even less to bufaline species (Ohashi, Rev Bras<br />

Reprod An, 299:31, 2007). Thus, the objective of this study was to<br />

determine the plasma levels of testosterone <strong>and</strong> <strong>and</strong>rostenedione in 35<br />

crossbred buffaloes (Murrah x Mediterranean), raised extensively in<br />

tropical region. The animals had 27.0 ± 4.08 months of age <strong>and</strong> 190.5 ±<br />

31.79 kg of carcass weight on average. The blood of animals was<br />

collected during the slaughter, transported under refrigeration to the<br />

laboratory (FMVZ/UNESP/Botucatu-Brazil) <strong>and</strong> centrifuged (2,500 g)<br />

for 15 minutes. The plasma was stored at -70 °C. The hormonal<br />

determinations were performed by the method of radioimmunoassay<br />

(RIA). The SAS statistical program was used to evaluate mean, st<strong>and</strong>ard<br />

deviation <strong>and</strong> Pearson’s correlation between the analyzed characteristics.<br />

The mean values <strong>and</strong> st<strong>and</strong>ard deviation of testosterone <strong>and</strong><br />

<strong>and</strong>rostenedione concentrations were respectively 124.98 ± 106.39 pg/ml<br />

<strong>and</strong> 221.14 - 272.10 ng/ml, results similar were found by Ohashi (Buffalo<br />

Journal, 12:313 -320, 1996) in crossbred buffaloes with 18 to 24 months.<br />

The testosterone concentration presented a positive correlation with body<br />

weight <strong>and</strong> <strong>and</strong>rostenedione concentration (p

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