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pharmacokinetic/pharmacodynamic (PK/PD) parameters. Six Holstein lactating cows with subclinical mastitis in at least one mammary quarter were selected. DNF 18% (Advocin ® ,Pfizer Inc, Argentina) was administered subcutaneously (10 mg/kg single dose). Blood and quarter milk samples were collected at different times until 96h after drug administration. Milk and plasma DNF concentrations were measured by HPLC with fluorescence detection.The MIC 90 was determined in 51 S. aureus strains to calculate PK/PD parameters. PK parameters were determined by non-compartmental analysis. Each mammary quarter was considered independently (n=24), since our purpose was to compare the findings in mastitic quarters (n=8) against healthy ones (n=16) and in quarters of high-producing (HP) cows (n=12) against lowproducing (LP) cows (n=12). The PK/PD parameters calculated were AUC 0-24h/MIC 90 (area under the concentration vs time until 24h/MIC for 90% of the isolates), AUC 0-∞/MIC 90 (AUC until infinity/MIC 90) and T>MIC 90 (time during which the concentrations exceed the MIC 90).DNF penetration into milk was fast. DNF concentrations determined in milk (C max=2.7±0.8 µg/mL) were higher than those found in plasma (C max=1.4±0.2 µg/mL) and milk bioavailability (F milk) was 4.2.C max in plasma occurred 1.2h postadministration, while C max in milk occurred 6h post-administration. T1/2 in milk (8.5h) was longer than T1/2 in plasma (4.4h). The PK parameters shows that milk concentrations decrease slower than plasma ones. According to the level of production, DNF was eliminated slower from quarters of LP cows. Concomitantly, the mean residence time (MRT) in milk from quarters of LP cows was longer than the MRT obtained in milk from quarters of HP cows. The C max and the C max/MIC 90 resulted larger in milk from quarters of LP cows. According to health status, it was observed that the MRT was longer in mastitic quarters. The level of production and the quarter health status could modify the PK and PK/PD parameters of DNF in milk of lactating cows treated parenterally. Consequently, we consider that therapeutic protocols should be adjusted according to susceptibility and the MIC of the bacteria involved and the level of production of the animal to be treated. Key words: danofloxacin, pharmacokinetic/pharmacodynamic, milk, mastitis, cows 406 Epidemiology of Staphyloccoccus aureus Intramammary Infection in Dairy Cows in Slovenia O. Podpecan 1 , P. Zrimsek 2 , M. Pate 3 , M. Ocepek 3 , I. Zdovc 3 , A. Pengov 3 1 Savinian Veterinary Policlinic, Ruminants, alec, Slovenia 2 Veterinary faculty Ljubljana, Clinic for Reproduction and Horses, Ljubljana, Slovenia 3 Veterinary faculty Ljubljana, Institute for Microbiology and Parasitology, Ljubljana, Slovenia The main objective of the study was to determine factors associated with epidemiology of S. aureus intrammamary infections in dairy cows. A total of 763 dairy cows and 2978 udder quarters from 42 herds were included in the study. Lactation number, body condition score and somatic cell counts (SCC) were calculated. Bacteriological determination of isolates and genotyping of S. aureus were performed using the Api-Staph test and pulsed-field gel electrophoresis (PFGE). Antibiotic resistance rates of S. aureus isolates were determined using the agar disk diffusion test. Statistical comparison of the results was performed using Kruskal- Wallis One Way Analysis of Variance on Ranks, Dunn´s test and Mann-Whitney-U test. The prevalence of S. aureus intramammary infection on a herd level was 33.7%. SCC in milk from udder quarters infected with S. aureus were significantly higher, irrespective of the lactation number, comparing with bacteriological negative udder quarters (P
Key words: semen kinematics, semen, fertility, CASA 409 Effect of Antioxidant Addition on in vitro Semen Quality and in vivo Fertility of Frozen-Thawed Bovine Semen M. Griga 1 , H. Nehring 2 , C. Leiding 1 1 Artificial Insemination Center BVN, Animal Reproduction and A.I, Neustadt Aisch, Germany 2 Institut für Fortpflanzung landwirtschaftlicher Nutztiere, Animal Reproduction and A.I, Berlin, Germany Reactive oxygen species (ROS) play a significant role in many of the sperm physiological processes and they also trigger many pathological processes. Favourable effects of antioxidant molecules on sperm motility have been reported in the literature. The objective of this study was to examine the influence of antioxidant molecules, content in to the Oxyfree ® Premix (IMV, Technologies, France), on the motility and fertility when added to bulls semen diluted with a egg yolk extender (Triladyl ® , Minitueb, Germany). Four ejaculate from each six Fleckvieh bulls were split into four fractions. Two fractions were diluted to a final concentration of 14 x 106 sperm/straw and another two fracton of 8 x 10 6 sperm/straw. One fraction with 14 and one with 8 x10 6 sperm/straw were diluted with extender containing Oxyfree. Frozen-thawed semen were evaluated for motility by a CASA system (SpermVision ® , Minitueb, Germany) 30 and 120 minutes after thawed and acrosomal integrity and spermatic membrane integrity by staining with fluorescein isothiocyanate-conjugated Arachis hypogea agglutinin (FITC-PNA) in combination with propidium iodide and analysed by flow cytometer (Beckman & Coulter, USA). ROS was determined by flow cytometer by staining with BODIPY 581/591 , dihydrorhodamine 123 (DHR) and dichlorofluorescin diacetate (DCFH), 15 minutes, 2 hours and 4 hours post-thaw and also 15 minutes with oxidative stress by adding of butyl hydroxide and hydrogen peroxide. Post-thawed semen evaluation schowed that motility (mean ± SD) was increased (p
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130. évfolyam Különszám II. Vol
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The World Buiatrics Congress (WBC)
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lood and liver tissue. Absolute vit
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RNS causes increased lipid peroxida
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An experiment was conducted to stud
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2 Hungarian Institute of Agricultur
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and plasma HCO 3 - increased from H
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Materials and Methods: Serum sample
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vitamin A stores in liver, the hypo
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andomly assigned to one of five tre
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observed in group II of the animals
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The aim of the monitoring was to co
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health group (HG) categories for an
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lactating cows is sparse. Lotan and
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491 Effect of Acarbose on Milk Yiel
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499 Secondary Hepatogenous Photosen
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they all died in a two to three day
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and HCO 3 level. The concentration
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amount of saponins was observed in
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Water was offered ad-libitum. Feed
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Se, I and Co) more than 500 blood s
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541 Variability of Blood Profile an
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evaluated, highly significantly gre
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we measured sensitivity of the enti
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were 0.17; 0.13 °C (Max.: 0.73). D
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calving pregnancy period were the b
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2 University of Nottingham, Departm
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animals) was treated with 5 mg enro
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In dairying, good management practi
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Somatic cell count (SCC) is one of
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This investigation was run in some
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eproductive data showed that cows f
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(3.6%) and others (Bacillus spp., N
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602 Coagulase Gene Polymorphism of
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2 Institute of Veterinary Bacteriol
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an important part of the pathogens
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4 Universidade de Sao Paulo, Clinic
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incubated for one hour at 37 oC. Af
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In Belgium, an increasing amount of
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Conclusion: It can be concluded tha
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calves recovered after 4 to 10 days
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disease. Thus losses were estimated
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635 Prevalence of the Pulmonary Les
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Conclusions The sanitation program
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Italy. After the sample examination
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Results: The cows sera were collect
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(Mb) as co-infecting agent is becom
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distributed over 13 different fatte
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main infection sources to the urban
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686 Characterisation of Cattle Orig
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using EPFC. The intradermal tubercu
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three cattle farms each. Leptospiro
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(cELISA) only to definitively confi
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1 Islamic Azad University of Shahre
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Financial support: CNPq, CAPES, FIN
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731 Detection and Investigation on
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necropsied, tissue samples were tak
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aseline values. The mean HCO 3, PCO
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immunization with either the curren
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decrease in animal aggregation in p
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747 Effect of Dietary Supplementati
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755 Study of Pulmonary Adenomatosis
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1 University of Erciyes, Faculty of
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2006 was observed a small decreasin
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for O69 strain), and clinical signs
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2 University of Veterinary Medicine
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794 Serum Haptoglobin and Fibrinoge
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complete random design (CRD). The r
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- Ca, Mg, K, and Ca:P ratio were lo
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818 Cerebral Coenurosis in a Goat:
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concentrations of the goat milk, in
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was significant and showed that bre
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cm long and 1/48 cm diameter. Middl
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circular external skeletal fixator
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847 Llama Anesthesia for Orthopedic
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28 Preliminary Results of a General
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Israeli commercial dairy herds cons
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109 Association between Johne’s D
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195 Changes in Plasma Beta-Carotene
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In the last year 2006, in the perio
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serious economic concern in the fat
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was a cumulative incidence rate of
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The level of antibodies produced in
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1096 Determination of Risk Indicato
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ParaCheck, CSL/Biocor, Australia (C
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1111 The Prevalence of Hock Lesions
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Objectives: To evaluate two differe
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1126 Relationships between Paratube
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1134 A Comparison between Two Calf
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The present work aimed to investiga
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University College Dublin, School o
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214 Cystic Ovarian Diseases in Dair
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inseminator were tested in regressi
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University of Nottingham, Animal He
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tissue (8,6 cm 2 ) was twice as lar
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libido in unmotivated males. Simila
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groups according to their diameter
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improve embryonic development are m
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in each sample). On day 0, 104 cows
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1 Warsaw University of Life Science
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and durable spermatozoa. Within the
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motility and CM integrity (p=0.869
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the median number of attempted moun
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909 The effect of “GnRH” and
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3 COOPEVOLIA, Centre Zootechnique,
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923 Production of Bovine Blackleg V
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Institute of Physiology and Sanocre
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2 University College Dublin, Animal
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(I.M.) 1 mg of estradiol cipionate
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Lameness results in major economic
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methods; ANOVA (with binary classif
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Initially two radiographs were obta
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number of positive faecal samples d
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Pfizer Animal Health, Veterinary Me
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Key words: calf, active immunity, p
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1 University of Camerino, Veterinar
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vaccinated (Group II) with Pastobov
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# Antigens Association in diarrhea
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1015 Prevalence of Eimeria Infectio
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group showed higher serum lactate c
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thymus in calves and heifers with c
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concentrations of calves with umbil
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The goal of this paper is to expose
Page 248 and 249: 187 Cooling Strategies to Improve M
Page 250 and 251: inspections, carried out by specifi
Page 252 and 253: The origin of Hungarian Grey cattle
Page 254 and 255: University of Warmia and Mazury in
Page 256 and 257: POSTER ABSTRACTS 1225 The Effect of
Page 258 and 259: multiple fracture (n=1, 1.3%). The
Page 260 and 261: position. A fusiform skin incision
Page 262 and 263: Conclusions: It is the first presen
Page 264 and 265: 1 Faculty of Veterinary Medicine, A
Page 266 and 267: of 101.55±4.94 mmHg. Afterwards th
Page 268 and 269: In Mexico, there is a wide variety
Page 270 and 271: Cardiovascular disorders are common
Page 272 and 273: Conclusion: The total contamination
Page 274 and 275: Material and methods: The trial inc
Page 276 and 277: detection of M. wenyonii infection.
Page 278 and 279: 390 Study on Attenuation Mechanisms
Page 280 and 281: POSTER ABSTRACTS 1152 Detection of
Page 282 and 283: digestion. It works only on Apicomp
Page 284 and 285: determined. In addition the surface
Page 286 and 287: Africa and Europe, which causes mor
Page 288 and 289: Results: On D0, the animals include
Page 290 and 291: addition of mineral salt, during 12
Page 292 and 293: digestion could be improved with im
Page 294 and 295: Ltd., Blandford Forum, UK). The air
Page 296 and 297: cattle ocular squamous cell carcino
Page 300 and 301: Bovine Immunodeficiency Virus (BIV)
Page 302 and 303: significantly lower in groups vacci
Page 304 and 305: 5.00+0.67 and 4.94+0.15 mEq/l in th
Page 306 and 307: potable water and food, avoiding st
Page 308 and 309: equal distributions at 08:00 and 16
Page 310 and 311: electrochemical properties of the a
Page 312 and 313: Selenium was discovered as an essen
Page 314 and 315: growth by measuring BW at 15-day in
Page 316 and 317: influence of different risk factors
Page 318 and 319: performances of primiparous cows ar
Page 320 and 321: anaerobes. Aerobic bacteria isolate
Page 322 and 323: P4 12 to 24 hours before calving is
Page 324 and 325: exists about Mb prevalence in dairy
Page 326 and 327: frequency of animals with BoHV anti
Page 328 and 329: esults, supported by a decrease in
Page 330 and 331: international drivers of change. Th
Page 332 and 333: The performance of two groups of no
Page 334 and 335: spasm is accompanied by kyphosis, w
Page 336 and 337: occurrence of a crisis of stiffness
Page 338 and 339: Notices 336 XXV. Jubilee World Buia
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