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(3.6%) and others (Bacillus spp., Nocardia spp, Candida spp.) (1.7%). These results demonstrate a significant share of minor pathogens beside contagious mastitis pathogens as S. aureus and S. agalactiae in mastitis incidents in Jalisco, Mexico. Key words: clinical and subclinical mastitis, bacterial pathogens, dairy cattle, Staphylococcus aureus, Streptococcus agalactiae 595 Evaluating Post-Milking Teat Dip Efficacy Using Somatic Cell Count Data A. Bradley 1,2 , J. Breen 1,2 , P. Janowicz 3 , M. McKinzie 3 , T. Hemling 3 1 University of Bristol, School of Veterinary Science, Bristol, United Kingdom 2 Quality Milk Management Services Ltd., Wells, United Kingdom 3 DeLaval, Kansas City, United States Objectives: Post milking teat disinfectants are traditionally assessed by identifying new intramammary infections (IMI) using regular individual quarter bacteriology. Somatic cell counts (SCC) are recognised as a proxy for IMI; quarters can be classified as “infected” or “uninfected” and new infections defined by movements across defined thresholds. The aim of this study was to compare the efficacy of two post milking teat disinfectants using both bacteriology and somatic cell counts, and to assess the utility of these two approaches. Materials and Methods: Two teat dip formulations (DeLaval Prima (0.5% hydrogen peroxide) and Hamra Blue (0.4% chlorhexidine)) were compared on three farms in the UK. Four hundred and fourteen cows were randomly allocated (ratio 1:1) to be dipped post milking with one of the two formulations. Cows were recruited over a 20 week period; 377 cows were eligible for inclusion in the subsequent analyses. Quarter milk samples were collected for bacteriology and SCC from all cows at enrolment and on completion of the study. During the study, quarters were sampled for SCC every 14 days. Bacteriology to confirm new IMIs was performed when quarters experienced a rise in SCC from below to above 100,000 cells/ml. All cases of clinical mastitis were sampled during the study in order to determine the cause of IMI. New IMIs were defined using various criteria; efficacy was evaluated using statistical protocols recommended by the National Mastitis Council (NMC). Results: Efficacy of the two products was compared; new IMIs were defined by movements across the 100,000 cells/ml threshold alone, and in combination with supporting bacteriology. Efficacy was also evaluated by comparing clinical mastitis incidence and the change in prevalence of IMI between entry and exit of quarters from the study. With the exception of the change in prevalence of infection between entry and exit from the study (due to a difference in minor pathogen IMI), none of the measures demonstrated a significant difference between the products. Analysis by various statistical protocols recommended by the NMC showed the experimental product (DeLaval Prima) was equivalent to the control product (Hamra Blue). Conclusion: The use of SCCs, with/without the judicious use of bacteriology offers a viable, cost effective alternative to the extensive use of bacteriology alone in assessing post-milking teat dip efficacy. 596 The Effectiveness of Cefalosporine+Kanamicine and Ampiciline Gentamicine + Dexametazone in the Treatment of Clinical Mastitis in the Livestock Bovine Milk in Mexico M. Posadas, L. Matus, O. Blanco, B. Pena Facultad de Medicina Veterinaria y Zootecnia-UNAM, Animal production, México DF, Mexico The present study was carried in Tizayuca Hidalgo, Mexico during July to September in order to determining the effectiveness of the treatment with the combination cefalexine monohydrate, kanamicine sulfate and ampiciline - gentamicine + dexametazone. We administered by the intrammary road for the solution of problems of clinical mastitis in bovine producing of milk. The sample was took in a population of 50 animals diagnosed with clinical mastitis and that they presented alterations in the milk besides that they obtained a grade 2 in test of California (CMT), which were integrated in two groups of 25 glands distributed randomly.The control group (A) it was conformed by 25 glands affected which was administered cefalexine monohydrate + kanamicine sulfate during 2 days for a intramamary road.The witness group (B) was conformed by 21 affected glands, to which was administered: ampiciline - gentamicine + dexametazone for a intramamary road with it forms to their medical prescription. They took samples of milk of the glands affected to carry out the bacteriological 64 XXV. Jubilee World Buiatrics Congress 2008 exam and to know the etiology.The isolated agents of the sample of milk of the two groups “A” and “B” was the following ones: Streptococcus agalactiae, Staphtlococcus aureus, Corynebacterium pseudotuberculosis, Pasterella spp., Moraxella spp., Eschericha coli, Staphylococcus epidermidis, Staphylococcus, saprophyticus, Serratia spp., Streptococcus uberis, Citrobacter spp., coagulase negative Staphylococci, Actinobacillus salpingitidis, Staphylococcus intermedius and Aeromona hydrophila. The results demonstrated that the combination cefalexine monohydrate and kanamicine sulfate, it had an effectiveness of 88% to control the presentation of clinical mastitis of the group “A” in comparison with the group “B” that was of 66%. In the statistical analysis they were not significant differences (P=.065>0.05). As for the presentation of alterations in the milk, the animals of the group “A” they recovered in 86% while the group “B” was of 76%. With regard to the test of California for the group “A” 82% of the glands didn’t present reaction some after the treatment while in the group “B” was of 68%, what allows to deduce that the cefalexine monohydrate and kanamicine sulfate is a highly advisable product for the treatment of clinical mastitis. 597 Effect of Anhydrous Cephalonium-Based Drug Indicated for Bovine Mastitis Treatment during the Dry Period on Phagocytosis Rates C. Batista, M. Blagitz, M. Azedo, C. Stricagnolo, M.C. Sucupira, A.M. Della Libera Faculdade de Medicina Veterinaria da Universidade de Sao Paulo, Clinica Medica, Sao Paulo, Brazil The dry period is considered a very vulnerable phase of lactation to mastitis and the phagocytosis mechanisms are fundamental for this period. The antibiotic therapy used for the treatment of dry cows acts on the elimination of existing intramammary infections, avoiding new infections and decreasing its incidence on the next lactation. An efficient drug must contain an adequate antimicrobial component in harmony with other eventual components and must be harmless to the organism’s defense mechanisms. Twenty-one paired samples, negative to bacteriological tests, were used to evaluate the influence of Cepravin ® , an anhydrous cephalonium-based drug indicated for the treatment of mastitis during the dry period, on the phagocytic function of milk leukocytes. In order to evaluate the cellular activity, leukocytes were isolated in adequate quality (viability) and quantity (concentration) and submitted to in vitro phagocytosis test with Zymosan (Saccaromyces cerevisae cell wall), after exposition to a solution containing the drug. The average phagocytosis rate for cells submitted to the treatment (64.43% ± 15.10) did not differ in relation to the average phagocytosis rate for the control group (58,33% ± 12,00). In addition, the coefficient of variation of phagocytosis rate obtained from cells submitted to the treatment (23.44) was not different from the coeficient found in control samples (20.57). The results suggest that the treatment, besides the antibiotic activity, does not interfere in the phagocytic function of milk leukocytes, which is fundamental for the defense of mammary gland, mainly during the dry period. Key words: mastitis, treatment, phagocytosis, dry period 598 Microbiological Evaluation, Somatic Cell and Differential Leukocytes Count in Relation to California Mastitis Test in Sante In’s Ewes M. Blagitz 1 , F. Souza 1 , V. Gomez 1 , C. Batista 1 , M. Azedo 1 , C. Strincagnolo 1 , N. Benites 2 , P. Melville 2 , M.C. Sucupira 1 , A. M. Della Libera 1 1 Faculty of Veterinary Medicine and Animal Husbandry FMVZ-USP, Clinica Medica, Sao Paulo, Brazil 2 Faculdade de Medicina Veterinaria da Universidade de Sao Paulo, Preventive Medicine and Public Health, Sao ppaulo, Brazil To evaluate the variation in microbiological profile, total somatic cell (SCC) and differential leukocytes counts (DSCC) in milk of Santa Ins ewes according to CMT scores, 170 milk samples were examined for microscopic somatic cell count (SCC), differential leukocytes count (DSCC), microbiological examination and their relationship with California mastitis test (CMT). On the 24.21% bacteriologically positive samples, 86.96% were coagulase negative staphylococci (CNS), and 13.04% were Streptococcus dysgalactiae. The mean (± Standard deviation) logarithm SCC of uninfected sheep’s gland was 5.01 (±0.87) and 5.93 (±0.94) (p
30.00%, 58.97% and 58.33% in negative, 1, 2, and 3 CMT scores respectively. The proportion of glands considered positive for CMT 1, 2 and 3 was 29.41%, 23.53% and 7.65% correspondingly. The results showed a higher somatic cell count on higher CMT scores, but no significant difference on 2 and 3 scores, thus the means (± Standard deviation) logarithm SCC according to CMT scores were 4.44 (+0.57), 5.21 (±0.46), 6.35 (±0.49), 6.52 (±0.73). Besides this, a positive and significant correlation (r = 0.822, p
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130. évfolyam Különszám II. Vol
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The World Buiatrics Congress (WBC)
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lood and liver tissue. Absolute vit
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RNS causes increased lipid peroxida
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An experiment was conducted to stud
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2 Hungarian Institute of Agricultur
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and plasma HCO 3 - increased from H
Page 16 and 17: Materials and Methods: Serum sample
Page 18 and 19: vitamin A stores in liver, the hypo
Page 20 and 21: andomly assigned to one of five tre
Page 22 and 23: observed in group II of the animals
Page 24 and 25: The aim of the monitoring was to co
Page 26 and 27: health group (HG) categories for an
Page 28 and 29: lactating cows is sparse. Lotan and
Page 30 and 31: 491 Effect of Acarbose on Milk Yiel
Page 32 and 33: 499 Secondary Hepatogenous Photosen
Page 34 and 35: they all died in a two to three day
Page 36 and 37: and HCO 3 level. The concentration
Page 38 and 39: amount of saponins was observed in
Page 40 and 41: Water was offered ad-libitum. Feed
Page 42 and 43: Se, I and Co) more than 500 blood s
Page 44 and 45: 541 Variability of Blood Profile an
Page 46 and 47: evaluated, highly significantly gre
Page 48 and 49: we measured sensitivity of the enti
Page 50 and 51: were 0.17; 0.13 °C (Max.: 0.73). D
Page 52 and 53: calving pregnancy period were the b
Page 54 and 55: 2 University of Nottingham, Departm
Page 56 and 57: animals) was treated with 5 mg enro
Page 58 and 59: In dairying, good management practi
Page 60 and 61: Somatic cell count (SCC) is one of
Page 62 and 63: This investigation was run in some
Page 64 and 65: eproductive data showed that cows f
Page 68 and 69: 602 Coagulase Gene Polymorphism of
Page 70 and 71: 2 Institute of Veterinary Bacteriol
Page 72 and 73: an important part of the pathogens
Page 74 and 75: 4 Universidade de Sao Paulo, Clinic
Page 76 and 77: incubated for one hour at 37 oC. Af
Page 78 and 79: In Belgium, an increasing amount of
Page 80 and 81: Conclusion: It can be concluded tha
Page 82 and 83: calves recovered after 4 to 10 days
Page 84 and 85: disease. Thus losses were estimated
Page 86 and 87: 635 Prevalence of the Pulmonary Les
Page 88 and 89: Conclusions The sanitation program
Page 90 and 91: Italy. After the sample examination
Page 92 and 93: Results: The cows sera were collect
Page 94 and 95: (Mb) as co-infecting agent is becom
Page 96 and 97: distributed over 13 different fatte
Page 98 and 99: main infection sources to the urban
Page 100 and 101: 686 Characterisation of Cattle Orig
Page 102 and 103: using EPFC. The intradermal tubercu
Page 104 and 105: three cattle farms each. Leptospiro
Page 106 and 107: (cELISA) only to definitively confi
Page 108 and 109: 1 Islamic Azad University of Shahre
Page 110 and 111: Financial support: CNPq, CAPES, FIN
Page 112 and 113: 731 Detection and Investigation on
Page 114 and 115: necropsied, tissue samples were tak
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aseline values. The mean HCO 3, PCO
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immunization with either the curren
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decrease in animal aggregation in p
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747 Effect of Dietary Supplementati
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755 Study of Pulmonary Adenomatosis
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1 University of Erciyes, Faculty of
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2006 was observed a small decreasin
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for O69 strain), and clinical signs
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2 University of Veterinary Medicine
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794 Serum Haptoglobin and Fibrinoge
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complete random design (CRD). The r
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- Ca, Mg, K, and Ca:P ratio were lo
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818 Cerebral Coenurosis in a Goat:
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concentrations of the goat milk, in
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was significant and showed that bre
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cm long and 1/48 cm diameter. Middl
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circular external skeletal fixator
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847 Llama Anesthesia for Orthopedic
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28 Preliminary Results of a General
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Israeli commercial dairy herds cons
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109 Association between Johne’s D
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195 Changes in Plasma Beta-Carotene
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In the last year 2006, in the perio
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serious economic concern in the fat
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was a cumulative incidence rate of
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The level of antibodies produced in
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1096 Determination of Risk Indicato
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ParaCheck, CSL/Biocor, Australia (C
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1111 The Prevalence of Hock Lesions
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Objectives: To evaluate two differe
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1126 Relationships between Paratube
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1134 A Comparison between Two Calf
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The present work aimed to investiga
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University College Dublin, School o
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214 Cystic Ovarian Diseases in Dair
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inseminator were tested in regressi
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University of Nottingham, Animal He
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tissue (8,6 cm 2 ) was twice as lar
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libido in unmotivated males. Simila
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groups according to their diameter
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improve embryonic development are m
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in each sample). On day 0, 104 cows
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1 Warsaw University of Life Science
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and durable spermatozoa. Within the
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motility and CM integrity (p=0.869
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the median number of attempted moun
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909 The effect of “GnRH” and
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3 COOPEVOLIA, Centre Zootechnique,
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923 Production of Bovine Blackleg V
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Institute of Physiology and Sanocre
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2 University College Dublin, Animal
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(I.M.) 1 mg of estradiol cipionate
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Lameness results in major economic
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methods; ANOVA (with binary classif
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Initially two radiographs were obta
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number of positive faecal samples d
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Pfizer Animal Health, Veterinary Me
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Key words: calf, active immunity, p
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1 University of Camerino, Veterinar
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vaccinated (Group II) with Pastobov
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# Antigens Association in diarrhea
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1015 Prevalence of Eimeria Infectio
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group showed higher serum lactate c
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thymus in calves and heifers with c
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concentrations of calves with umbil
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The goal of this paper is to expose
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187 Cooling Strategies to Improve M
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inspections, carried out by specifi
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The origin of Hungarian Grey cattle
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University of Warmia and Mazury in
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POSTER ABSTRACTS 1225 The Effect of
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multiple fracture (n=1, 1.3%). The
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position. A fusiform skin incision
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Conclusions: It is the first presen
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1 Faculty of Veterinary Medicine, A
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of 101.55±4.94 mmHg. Afterwards th
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In Mexico, there is a wide variety
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Cardiovascular disorders are common
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Conclusion: The total contamination
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Material and methods: The trial inc
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detection of M. wenyonii infection.
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390 Study on Attenuation Mechanisms
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POSTER ABSTRACTS 1152 Detection of
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digestion. It works only on Apicomp
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determined. In addition the surface
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Africa and Europe, which causes mor
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Results: On D0, the animals include
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addition of mineral salt, during 12
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digestion could be improved with im
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Ltd., Blandford Forum, UK). The air
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cattle ocular squamous cell carcino
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pharmacokinetic/pharmacodynamic (PK
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Bovine Immunodeficiency Virus (BIV)
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significantly lower in groups vacci
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5.00+0.67 and 4.94+0.15 mEq/l in th
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potable water and food, avoiding st
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equal distributions at 08:00 and 16
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electrochemical properties of the a
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Selenium was discovered as an essen
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growth by measuring BW at 15-day in
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influence of different risk factors
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performances of primiparous cows ar
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anaerobes. Aerobic bacteria isolate
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P4 12 to 24 hours before calving is
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exists about Mb prevalence in dairy
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frequency of animals with BoHV anti
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esults, supported by a decrease in
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international drivers of change. Th
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The performance of two groups of no
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spasm is accompanied by kyphosis, w
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occurrence of a crisis of stiffness
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Notices 336 XXV. Jubilee World Buia
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