Oral and Poster Abstracts

thermography. Two groups of 14 buffaloes were milked at 42 kPa, high
vacuum (HV) and, after 3 weeks of adaptation to progressively lower
vacuum levels, at 36 kPa (LV), using the same pulsation parameters (60
cycles/min and 65:35 ratio). Milking was carried-out at intervals of 9
hours (daytime) and 15 hours (over-night). Tests were performed in a
28+28 units parallel parlour with low line milking system equipped with
light weight clusters (1.78 kg), automatic cluster removers and
electronic herd management system. Thermographic images (Flir
System, ThermaCam P25) of posterior udder area (PUA) and teats
(base-teat - BT; middle-teat - MT and tip-teat - TT) were taken premilking
(PM), during milking (M), immediately after milking (IAM)
and up to 5 minutes after milking (AM+). Temperatures were recovered
by processing the thermoimages in ThermaCam Researcher Basic 2.8
Software. The results of vacuum level effects on teat tissue temperature
show evident differences among BT and TT, and among MT and TT in
all stages. Moreover interesting is the temperature difference between
LV and HV levels at PM and IAM: relevant gap is showed in HV level
(2.67 °C) compared to LV (1.79 °C), underlining a greater blood flow
arrangement and smaller distress of teat. Though being an introductive
work, this data set suggests an important role of thermography to
monitor the effects of milking procedures on udder and teat.
838 Serum Levels of Gamma Glutamyl Transferase (GGT) and
Aspartate Aminotransferase (AST) in Murrah Buffaloes
V. Gomes 1 , K. Madureira 1 , K. Leonel 1 , M. Blagitz 2 , S. Kitamura 3 ,
A. Gomes 4 , F. Benesi 2
1 Faculdades Anhanguera, Medicina Veterinária, Leme, Brazil
2 Universidade de Sao Paulo, Clinica Medica, Sao Paulo, Brazil
3
Universidade Anhembi Morumbi, Medicina Veterinária, Sao Paulo,
Brazil
4
Universidade Estadual do Norte do Parana, Medicina Veterinária,
Bandeirantes, Brazil
Although buffaloes are affected by several hepatic diseases, diagnosis
of these conditions only based on liver examination is difficult. Due to
this fact, the use of complementary exams, such as serum activity of
liver enzymes GGT and AST is very important. However, there are no
reference values determined for buffaloes that would aid the
interpretation of the results. Therefore, the objective of the present
study was to determine reference values and to assess the influence of
age on serum activity of GGT and AST in Murrah buffaloes bred in Sao
Paulo, Brazil. Serum samples collected from 125 clinically healthy
buffaloes and were placed into 4 experimental groups, according to the
age: 0 to 3 months of age (Group 1); 4 to 6 months of age (Group 2); 7
to 12 months of age (Group 3); and over 1 year of age (Group 4). GGT
and AST serum activities were evaluated using commercial kits in an
automatic biochemical analyzer at 405nm. GGT serum activity was
equal to 35.9; 19.6; 25.1; and 33.3 UI/L, respectively, in animals of
Groups 1, 2, 3 and 4. Activity was significantly greater in the first
months of life. AST serum activity, on the other hand, was significantly
greater in the first months of life (92.3 UI/L), increasing to 128, 177 and
143 UI/L as animals grew older in groups 2, 3 and 4, respectively.
Therefore, it was concluded that age is a physiological factor that
causes variations in the serum activity of liver enzymes GGT and AST.
Besides, reference values were determined for the enzymes, which is
an excellent aid to the clinical diagnosis of liver diseases in domestic
animals.
Key words: Murrah buffaloes, blood, gamma glutamyl transferase,
aspartate aminotransferase
839 Total Protein, Albumin and Globulin Serum Concentrations
in Murrah Buffaloes. Reference Values and Influence of Age
V. Gomes 1 , K. Madureira 1 , K. Leonel 1 , M. Silva 1 , M. Blagitz 2 ,
A. Gomes 3 , S. Kitamura 4 , F. Benesi 2
1
Faculdades Anhanguera, Medicina Veterinária, Leme, Brazil
2
Universidade de Sao Paulo, Clinica Medica, Sao Paulo, Brazil
3
Universidade Estadual do Norte do Parana, Medicina Veterinária,
Bandeirantes, Brazil
4
Universidade Anhembi Morumbi, Medicina Veterinária, Sao Paulo,
Brazil
Proteins are indispensable to life due to their biological significance
and to the various functions they perform in the organism. Because of
this, the evaluation of protein serum concentration is an important tool
in clinical diagnosis. Although it is important to know the physiological
biochemical profile for an adequate interpretation of results when
serum proteins are evaluated, there are few studies on the subject
involving buffaloes. In the present study, serum concentrations of total
protein, albumin and globulin were determined in 125 healthy Murrah
buffaloes. Animals were divided into 4 experimental groups, according
to the age: 0 to 3 months of age (Group 1); 4 to 6 months of age (Group
2); 7 to 12 months of age (Group 3); and over 1 year of age (Group 4).
Serum concentration of total proteins was carried out by means of the
biuret method, whereas serum albumin was determined by means of
bromocresol green. Globulin concentration was determined by
subtracting individual values obtained for total protein and albumin.
Values were expressed in g/dL. Mean concentration of serum protein
was greater in animals of group 1 (8.2 g/dl) and 4 (7.7 g/dL), and lower
in animals of group 2 (7.0 g/dL) and 3 (6.9 g/dL). Albumin
concentration was equal to 2.4; 2.2; 2.1 and 2.5g/dL in animals of
groups 1, 2, 3 and 4, respectively. Concentrations were greater in
groups 1 and 4. Globulin concentration was equal to 5.8; 4.7; 4.7; and
5.1, in groups 1, to 4, respectively. Therefore, age seemed to influence
the biochemical characteristics studied, with greater total protein,
albumin and globulin concentrations mainly observed in group 1.
Key words: Murrah buffaloes, total protein, albumin and globulin
840 A Survey on some Bacteriological and Pathological Aspects of
Buffalo Liver Abscesses
A. Ghadrdan-Mashhadi 1 , M. Jamshidian 2 , K. Gheysar-Beigi 3
1
Faculty of Veterinary Medicine, Shahid Chamran University,
Department of Clinical Sciences, Ahvaz, Iran
2
Faculty of Veterinary Medicine, Shahid Chamran University,
Department of Pathobiology, Ahvaz, Iran
3
Faculty of Veterinary Medicine, Shahid Chamran University, Ahvaz,
Iran
Local suppurative infections of the liver do not cause clinical signs of
hepatic dysfunction unless they are particularly massive or extensively
metastatic, however it sometimes causes to weight loss. Also, they do
cause significant losses in feedlot and grain-fed animals because of
rejection of the affected liver at the abattoir. This study was carried out
to find the occurrence of liver abscesses in buffaloes slaughtered in
Ahvaz in 2003. The animals (n: 503) were selected from two sexes and
divided to three age groups (group A: less than 2.5 year, group B: 2.5-
5 years, and group C: more than 5 years). If the abscesses were present,
the location and sizes would be recorded and then sampling for
bacterial culture was done. Results showed that 5 buffaloes (0.99%)
had liver abscesses. According to presence of abscesses, there was no
significant difference between two sexes and three age groups. Most of
the abscesses were found in right lobes (40%) and in diaphragmatic
surface (60%) of livers. The following bacteria were isolated: A.
pyogenes (3 cases) and S. epidermidis (1 case). In one case the abscess
was sterile.
841 The Anatomy of Buffals Cardiac Veins
HA. Karimi 1 , GH. Ardalani 2 , AH. Zadeh Kokia
1 Faculty of Veterinary Medicine, University of Tabriz, Basic
Sciences, Tabriz, Iran
2 Faculty of Veterinary Medicine, Islamic Azad University of
Shahrekord, Basic Sciences, Urmia, Iran
In this research anatomical structure of coronary vein and type of low
oxygen blood drainage into right atrium was studied in Gharbi
Azarbayjan buffalo heart. 20 healthy buffaloes’ hearts and healthy cows’
hearts are collected from Tabriz industrial slaughter house. Buffaloes’
hearts are used for studying coronary veins and cows’ hearts are used for
comparating to buffaloes’ hearts. Anatomical structure of buffalo
cardiatic veins (coronary veins) are detected by injection contraste meida
such as Barium Sulphat and magnulin and radiograph was prepared. Also
the hearts were injected by coloured latex, rodopass and ejllatine and this
injected buffaloes’ hearts are dissected and compared to cows’ hearts.
Obtained results of the survey are showed that buffaloes cardiatic blood is
drining into right atrium by two veins, great cardiatic veins and middle
cardiatic veins. Great cardiatic vein lies on left longitudinal groove. It was
parallel to left longitudinal branch of left coronary artery. Great cardiatic
vein arises a circumflex branch that lies on coronary groove between left
atrium and left ventricle. Great cardiatic vein receives blood from left
atrium and left ventricle and it drains into coronary sinus. Obtained results
of coronary sinus biometry are showed that coronary sinus has about 4/5
Sheep, Goat and Buffaloes 143