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2 University College Dublin, Animal Health and Welfare, Dublin, Ireland A total of 46 Irish Holstein-Friesian spring-calving cows were blocked [lactation number (1 to 8), body condition, body weight, calving date, predicted milk yield] and allocated to two management environments [cubicle housing with a total mixed ration pre- and postpartum (HOUSED) and rotational pasture with grass silage preand grass and concentrate postpartum (GRASS)]. The occurrence of reproductive disorders [dystocia, retained foetal membranes and puerperal metritis, 0-10 days in milk (DIM) and endometritis (MetricheckTM) 35-49 DIM], the commencement of luteal activity (CLA) (from thrice weekly milk sampling for progesterone analysis, EIA) and reproductive performance following a seasonal breeding period (98 days), were recorded. Proportions were analysed using Fisher’s exact or chi-square while the collated records for continuous variables were analysed using Wilcoxon’s or T-test in SAS, as appropriate. The raw average milk production for the treatments was of 6,186 kg vs. 7,299 kg for GRASS and HOUSED cows, respectively. Cows managed in the GRASS environment had a lower incidence of dystocia (4.4 vs 17.4%, P=0.08), puerperal metritis (17.4 vs 47.8%, P=0.06), endometritis (9.1 vs 31.8%, P=0.07) and an earlier CLA (median, and interquintile range: 23, 20- 31 vs 34, 28-50 days, P=0.09). Overall, reproductive performance was suboptimal and did not differ significantly between groups. However, it is posited that the lower incidence of uterine disorders and earlier CLA of the GRASS cows may have contributed to their numerically higher submission rate (82.6 vs 60.9%, P=0.19), shorter calving to service (70 vs 75.7 days, P=0.38) and calving to conception (102 vs 107.1 days, P=0.29) intervals and higher final pregnancy rate (73.9 vs 60.9%, P=0.53). Though this is a biologically plausible explanation, limited experimental units may have reduced the probability of detecting these numerical differences as statistically significant. It is speculated that treatmentinduced differences in body condition loss pre- and postpartum and differential peripartum immune suppression may have contributed to these findings. In conclusion, cows managed in a GRASS environment appeared to have an earlier onset of uterine involution and luteal activity and this appeared to be associated with better reproductive performance however; the limited scale of this study precludes robust inference. Key words: uterine infection, reproductive performance, pasture vs TMR systems 938 Apoptosis, Placental Maturation and Placental Retention in Bos Taurus E. Martins 1 , A. Vasconcelos 1 , A. Marques Jr 2 , J. Ferreira Neto 2 , J. Nunes 2 , V. Martins 1 , K. Meça 1 1 Instituto de Ciencias Biológicas UFMG, General Pathology, Belo Horizonte, Brazil 2 Escola de Veterinária UFMG, Departamento de Clinica e Cirurgia Veterinaria, Belo Horizonte, Brazil Apoptosis is an important event for the homeostasis and placental maturation. A decreased cellularity in placentome close to parturition suggests that placental release is somehow associated with that. Here, the role of apoptosis in placental maturation was evaluated in 42 Holstein puerperal cows, distributed in 3 treatments: parturition to term (Group I, n= 22); parturition to term and retained placenta (Group II, n=10) and dexamethasone-induced parturition 5 days before the predicted time (Group III, n=10). Placentomes were collected and processed. Slides stained by Shorr and MGP were submitted to morphometry to count caruncular epithelial, trophoblast binucleated and apoptotic cells. Apoptosis was also approached by the TUNEL, ELISA (to detect free histones), DNA electrophoresis (to confirm the internucleosomal fragmentation) and transmission electron microscopy. Caruncular cells along 100 µm of epithelium did not differ between groups I (3,59±0,59) and II (4,28±0,71) (p>0.10). However, group I presented a number of caruncular cells smaller than group III (4,75 ± 1,56) (p0.01) than in groups II (4,84±10,84) and III (5,02±11,18). ELISA results were similar to the morphometric ones, except that groups III and I were not different (p
940 The Effect of Linseed Based Inert Fat Supplementation on Reproductive and Metabolic Performance in Holstein-Friesian Cows M. Keresztes 1 , G. Sassi 2 , V. Faigl 1 , M. Kulcsár 1 , H. Fébel 3 , M. Mézes 4 , Gy. Gábor 3 , O. Szenci 1,2 , Gy. Huszenicza 1 1 Szent István University Faculty of Veterinary Science, Reproduction, Budapest, Hungary 2 Szent István University Faculty of Veterinary Science, Large Animal Clinic, Budapest, Hungary 3 Research Institute for Animal Breeding and Nutrition, Herceghalom, Hungary 4 Szent István University Faculty of Agricultural and Environmental Sciences, Department of Animal Nutrition, Gödöll[, Hungary Feeding rumen-protected fatty acids to ruminants - particularly if it is rich in polyunsaturated fatty acids (PUFA) - can influence reproductive activity through a variety of mechanisms. Both n-6 and n-3 PUFAs can stimulate the ovarian follicular growth, the luteal function and the steroid metabolism of the animals. The objective of this work was to determine the effect of a linseed based inert fat supplementation rich in linoleic (C18:2, n-6) and linolenic acid (C18:3, n-3) on metabolites and hormones in plasma, as well as on resumption of ovarian activity during early lactation. Forty-nine multiparous (2,73 ± 0,45 BCS) Holstein-Friesian cows were assigned in Control (n=25) and fat-supplemented (FS; n=24) groups. Beginning 1 day post partum rumen protected fat based on linseed oil was added to the TMR diet of the Treated group, throughout 90 days of the lactation. Metabolizable protein and net energy for lactation concentrations were similar in the 2 diets. Blood samples were taken on the 1, 2, 5, 10-11 postpartum weeks for quantification of ‚OH-butyrate (BHB), non esterified fatty acids (NEFA), urea, insulin, insulin-like growth hormone factor I (IGF-I), 3’,3,5triiodtironine (T3) and thyroxine (T4) levels. Milk samples were taken three times a week from the parturition until the 7 week for progesterone (P4) concentration. Average daily milk production in the first 180 pp day was higher in the treated group (30.96± 5 kg/d vs. 26.66±4.6 kg/d; P
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130. évfolyam Különszám II. Vol
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The World Buiatrics Congress (WBC)
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lood and liver tissue. Absolute vit
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RNS causes increased lipid peroxida
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An experiment was conducted to stud
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2 Hungarian Institute of Agricultur
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and plasma HCO 3 - increased from H
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Materials and Methods: Serum sample
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vitamin A stores in liver, the hypo
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andomly assigned to one of five tre
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observed in group II of the animals
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The aim of the monitoring was to co
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health group (HG) categories for an
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lactating cows is sparse. Lotan and
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491 Effect of Acarbose on Milk Yiel
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499 Secondary Hepatogenous Photosen
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they all died in a two to three day
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and HCO 3 level. The concentration
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amount of saponins was observed in
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Water was offered ad-libitum. Feed
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Se, I and Co) more than 500 blood s
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541 Variability of Blood Profile an
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evaluated, highly significantly gre
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we measured sensitivity of the enti
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were 0.17; 0.13 °C (Max.: 0.73). D
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calving pregnancy period were the b
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2 University of Nottingham, Departm
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animals) was treated with 5 mg enro
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In dairying, good management practi
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Somatic cell count (SCC) is one of
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This investigation was run in some
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eproductive data showed that cows f
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(3.6%) and others (Bacillus spp., N
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602 Coagulase Gene Polymorphism of
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2 Institute of Veterinary Bacteriol
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an important part of the pathogens
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4 Universidade de Sao Paulo, Clinic
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incubated for one hour at 37 oC. Af
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In Belgium, an increasing amount of
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Conclusion: It can be concluded tha
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calves recovered after 4 to 10 days
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disease. Thus losses were estimated
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635 Prevalence of the Pulmonary Les
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Conclusions The sanitation program
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Italy. After the sample examination
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Results: The cows sera were collect
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(Mb) as co-infecting agent is becom
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distributed over 13 different fatte
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main infection sources to the urban
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686 Characterisation of Cattle Orig
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using EPFC. The intradermal tubercu
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three cattle farms each. Leptospiro
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(cELISA) only to definitively confi
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1 Islamic Azad University of Shahre
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Financial support: CNPq, CAPES, FIN
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731 Detection and Investigation on
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necropsied, tissue samples were tak
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aseline values. The mean HCO 3, PCO
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immunization with either the curren
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decrease in animal aggregation in p
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747 Effect of Dietary Supplementati
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755 Study of Pulmonary Adenomatosis
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1 University of Erciyes, Faculty of
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2006 was observed a small decreasin
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for O69 strain), and clinical signs
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2 University of Veterinary Medicine
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794 Serum Haptoglobin and Fibrinoge
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complete random design (CRD). The r
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- Ca, Mg, K, and Ca:P ratio were lo
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818 Cerebral Coenurosis in a Goat:
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concentrations of the goat milk, in
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was significant and showed that bre
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cm long and 1/48 cm diameter. Middl
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circular external skeletal fixator
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847 Llama Anesthesia for Orthopedic
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28 Preliminary Results of a General
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Israeli commercial dairy herds cons
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109 Association between Johne’s D
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195 Changes in Plasma Beta-Carotene
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In the last year 2006, in the perio
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serious economic concern in the fat
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was a cumulative incidence rate of
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Page 168 and 169: 1096 Determination of Risk Indicato
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Page 172 and 173: 1111 The Prevalence of Hock Lesions
Page 174 and 175: Objectives: To evaluate two differe
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Page 178 and 179: 1134 A Comparison between Two Calf
Page 180 and 181: The present work aimed to investiga
Page 182 and 183: University College Dublin, School o
Page 184 and 185: 214 Cystic Ovarian Diseases in Dair
Page 186 and 187: inseminator were tested in regressi
Page 188 and 189: University of Nottingham, Animal He
Page 190 and 191: tissue (8,6 cm 2 ) was twice as lar
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Page 196 and 197: improve embryonic development are m
Page 198 and 199: in each sample). On day 0, 104 cows
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Page 202 and 203: and durable spermatozoa. Within the
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Page 208 and 209: 909 The effect of “GnRH” and
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Page 212 and 213: 923 Production of Bovine Blackleg V
Page 214 and 215: Institute of Physiology and Sanocre
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Page 220 and 221: Lameness results in major economic
Page 222 and 223: methods; ANOVA (with binary classif
Page 224 and 225: Initially two radiographs were obta
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Page 228 and 229: Pfizer Animal Health, Veterinary Me
Page 230 and 231: Key words: calf, active immunity, p
Page 232 and 233: 1 University of Camerino, Veterinar
Page 234 and 235: vaccinated (Group II) with Pastobov
Page 236 and 237: # Antigens Association in diarrhea
Page 238 and 239: 1015 Prevalence of Eimeria Infectio
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Page 246 and 247: The goal of this paper is to expose
Page 248 and 249: 187 Cooling Strategies to Improve M
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Page 254 and 255: University of Warmia and Mazury in
Page 256 and 257: POSTER ABSTRACTS 1225 The Effect of
Page 258 and 259: multiple fracture (n=1, 1.3%). The
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Page 262 and 263: Conclusions: It is the first presen
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of 101.55±4.94 mmHg. Afterwards th
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In Mexico, there is a wide variety
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Cardiovascular disorders are common
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Conclusion: The total contamination
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Material and methods: The trial inc
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detection of M. wenyonii infection.
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390 Study on Attenuation Mechanisms
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POSTER ABSTRACTS 1152 Detection of
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digestion. It works only on Apicomp
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determined. In addition the surface
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Africa and Europe, which causes mor
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Results: On D0, the animals include
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addition of mineral salt, during 12
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digestion could be improved with im
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Ltd., Blandford Forum, UK). The air
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cattle ocular squamous cell carcino
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pharmacokinetic/pharmacodynamic (PK
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Bovine Immunodeficiency Virus (BIV)
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significantly lower in groups vacci
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5.00+0.67 and 4.94+0.15 mEq/l in th
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potable water and food, avoiding st
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equal distributions at 08:00 and 16
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electrochemical properties of the a
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Selenium was discovered as an essen
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growth by measuring BW at 15-day in
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influence of different risk factors
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performances of primiparous cows ar
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anaerobes. Aerobic bacteria isolate
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P4 12 to 24 hours before calving is
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exists about Mb prevalence in dairy
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frequency of animals with BoHV anti
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esults, supported by a decrease in
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international drivers of change. Th
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The performance of two groups of no
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spasm is accompanied by kyphosis, w
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occurrence of a crisis of stiffness
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Notices 336 XXV. Jubilee World Buia
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