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exists about Mb prevalence in dairy herds in France. A national serological survey previously showed that in French beef cattle an average of 7% of animals and 28 to 90% of the herds, according to the department, have been infected by Mb. Two recent studies were conducted to try to determine the prevalence of Mb udder infection in dairy cattle. The first one was implemented with the aim of estimating the Mb prevalence in the 1522 herds of a milk cooperative covering six southeastern French departments. The number of herds to be tested (n=345) was calculated based on an expected Mb infection prevalence rate of 2% (by reference to US data). The second one was conducted over a period of 12 months, based on 280 dairy herds coming from the same region, in order to estimate the prevalence of Mb in clinical mastitis. Each month, only one clinical mastitis milk sample was allowed to be collected from the different herds. Mb search was carried out by i) culture and then identification by dot immunobinding and ii) PCR directly performed on samples (kit LSI-Lissieu-France). Occurrence for M. canadense, M. bovigenitalium and M. alkalescens was also tested. The results demonstrated that the prevalence for Mb infection in these dairy herds was lower than the 2% expected. Both surveys were preliminary studies and will need to be supported by further investigations in order to confirm our results in other areas of France with different dairy farming systems. Key words: Mycoplasma bovis, milk, dairy herds, prevalence 205 Epidemiological Surveillance of Ruminant Mycoplamosis in France F. Poumarat 1 , MA. Arcangioli 2 , M. Chazel 1 , D. Le Grand 2 , P. Gaurivaud 1 , F. Tardy 1 , D. Calavas 1 1 AFSSA, Ruminant Mycoplasmosis, Lyon, France 2 Ecole Vétérinaire, Ruminant Mycoplasmosis, Marcy l'Etoile, Lyon, France A network of diagnosis laboratories coordinated by the French Food Safety Agency (AFSSA) allows a continuous epidemiological surveillance of ruminant mycoplasmosis throughout French territory. The aims are: i) to identify mycoplasmas isolated from the ruminants; ii) to follow the evolution of these mycoplasmosis and to detect emergence of new species or variants; iii) to identify any re-emergence of Contagious Bovine Peripneumoniae (CCBP); iv) to share scientific information and technical knowledge relative to the ruminant mycoplasmosis; v) to collect and keep representative collection of isolates. Mycoplasmas are first isolated from field samples in veterinary diagnostic laboratories and then send to AFSSA Lyon. Species identification is performed by dot immunobinding assay, and PCR is used to confirm if necessary. Then, for unidentified strains, conclusion is obtained by sequencing of 16S RNA genes and comparison to data bases. Between 2003 and 2007, about 2000 mycoplasmas isolates from animals originating from more than 70 out 208 Prevalence of Salmonella as Cause of Abortions in Cattle in Southwest Ireland and Protective Effect of Vaccinations J. Crilly 1 , E. Power 2 , C. Sanchez-Miguel 2 , D. Dufe 3 , B. Makoschey 4 1 Dairy Production Research Centre, Cork, Ireland 2 Regional Veterinary Laboratory, Cork, Ireland 3 Intervet-Schering-Plough, Regulatory Affairs, Boxmeer, Netherlands 4 Intervet-Schering-Plough, Int. Marketing, Boxmeer, Netherlands Objectives: The purpose of this study was to estimate the prevalence rates of Salmonella Dublin (S. Dublin) as the cause of bovine abortions in southwest Ireland and to quantify the protective effects of S. dublin vaccination under field conditions. Materials and Methods: Pre-existing data on submissions from aborted and stillborn calves to the Cork Regional Veterinary Laboratory from 1989 to 2006 were used in the analysis. The material was tested for Salmonella spp by standard bacteriological culture. By linking the vaccination history of individual submissions to the post-mortem bacteriological findings, it was possible to assess 322 XXV. Jubilee World Buiatrics Congress 2008 Abortions Workshop of 96 French departments were identified. 1100 from cattle were analysed over the period. 95% came from bronchopneumonia affecting mainly young animals (87%) and 5% from arthritis and mastitis. Mycoplasma (M.) bovis was the most commonly identified pathogen (43%), singly or in mixed culture (20%). Otherwise M. bovirhinis and M. arginini were regularly found respectively (23% and 13%) but are thought to be only opportunistic. M. bovigenitalium, M. canadense, A. laidlawii and M. mycoides subsp. mycoides biotype Large Colony were sporadically encountered. Few isolates of M. canis and M. alkalescens that has been suggested to be cause of respiratory disease, arthritis and mastitis in cattle have been isolated but these mycoplasmas has not shown in France the epizootic increasing that has been reported recently in UK. No strain of M. mycoides subsp. mycoides biotype Small Colony, the agent of CBPP, was detected either in cattle or in small ruminants. These results underscore the importance in France of M. bovis in cattle pneumonia. But M. bovis mastitis incidence seems to be low as confirmed by other targeted surveys, only 10 outbreaks have been identified for 10 years. Key words: ruminants, mycoplasma, epidemiology, network, surveillance 206 Prevalence and Control of Mycoplasma bovis in Europe R. Nicholas 1 , E. Raedelli 2 , M. Luini 3 , G. Loria 4 , R. Ayling 1 , E. Scanziani 2 1 Veterinary Laboratories Agency, SEB, Addlestone, United Kingdom 2 University of Milan, Milan, Italy 3 Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna Brescia, Milan, Italy 4 Veterinary Laboratories Agency, SEB, Addlestone, United Kingdom 5 Veterinary Laboratories Agency, SEB, Addlestone, United Kingdom 6 University of Milan, Milan, Italy Mycoplasma bovis is a major cause of disease in both growing and adult cattle. Pneumonia, mastitis, arthritis and otitis have all been associated with mycoplasma infection, none of which respond well to chemotherapy. In the UK serological screening of pneumonic herds indicates that M. bovis is associated with approximately a quarter of all outbreaks in calves; this mycoplasma is also the most commonly isolated pathogen from pneumonic lungs. Since its first introduction into Ireland in the mid 1990s, M. bovis has also become a frequent isolate from pneumonic lungs. In a recent study of cattle rearing regions in Northern Italy, M. bovis was the most common bacterial pathogen involved in bovine pneumonia; all veal calves and three quarters of all beef cattle were seropositive to M. bovis. A commercial vaccine still appears a long way from availability in Europe but autogenous vaccines, which are being used increasingly, may help to bridge this gap in the short term. Key words: Mycoplasma bovis, pneumonia, autogenous vaccine the benefit of S. dublin vaccinations under field conditions. All vaccination were performed with inactivated S. Dublin vaccines (Intervet International, The Netherlands). More than 16,000 submissions from cases of bovine abortions were made over the 18 year study period. Results: Apart from a small number of cases, which were unsuitable for bacteriological examination due to advanced autolysis, culture results were available for S. Dublin for most of the submissions. Of all samples examined for S. Dublin, 9.9% were culture positive. About 25% of the submissions were from cows vaccinated against S. Dublin, 25% cows not vaccinated against this pathogen and 50% with unknown vaccination status. The level of vaccination against S. Dublin clearly increased during the study period. The time series of abortions caused by S. Dublin showed evidence of two components, namely a background prevalence with periods where levels increase two fold or more. Conclusions: In submissions from vaccinated cows, the relative risk of being S. Dublin culture positive was consistently lower than for submissions from unvaccinated animals or animals with unknown
vaccination status (average 1989-2007: 7% for vaccinated animals versus 12% for unvaccinated animals). The benefit of salmonella vaccination appeared greater from 1996 onwards. The relative risk of being S. Dublin culture positive was only 5% for submissions from vaccinated animals after 1995 compared to 13% for submissions from unvaccinated animals in the same period. 209 Sero-Epidemiology of Brucellosis in Goats at Rakh Khaire Wala Farm District Layyah, Pakistan M. Arshad 1 , M. Munir 1 , Z. Abbas 2 1 University of Agriculture, Department of Microbiology, Faisalabad- 38040,, Pakistan 2 University of Agricuture, Department of Parasitology, Faisalabad, Pakistan Introduction: Brucellosis is responsible for considerable economic losses due to abortion, low milk production and infertility in goats. It also has a zoonotic importance that plays a significant role in the national economy and the public health of many parts of the world particularly in developing countries. Keeping in view the economic and zoonotic importance of this disease, the project had been planned to study the sero-prevalence of brucellosis in goats at Rakh Khaire Wala Farm, District Layyah, Pakistan. Materials And Methods: A total of five hundred (500) serum samples were collected randomly from goat breeds (Teddy, Pak 239 The Present and the Future of IBR Control P. Franken Animal Health Service Deventer, Cattle Health Service, Deventer, Netherlands The development of a marker vaccine against IBR, has opened the way for the eradication of IBR also in high prevalence and high density area's. A number of European countries have started or will start eradication programmes based on the use of this vaccine. Actually there is a lot of knowledge and experience about control and eradication of IBR in different epidemiological situations.An overview will be given of the present status in Europe and the needs for international trade of animals and genetic material will be stated. Key words: IBR, marker vaccine, control, future 240 Infectious Bovine Rhinotracheitis and the Epidemiological Role of the other Ruminant Species J. Thiry, B. Muylkens, E. Thiry University of Liege - Faculty of Veterinary Medicine, Department of Infectious and Parasitic Diseases - Virology and Viral Diseases, Liege, Belgium See in the Supplement I. 241 Pitfalls in IBR Diagnosis in the Field M. Beer, P. Koenig Friedrich-Loeffler-Institut, Institute of Diagnostic Virology, Greifswald-Insel Riems, Germany BHV-1 (IBR/IPV) control is based on marker vaccination and/or removal of infected animals which is mainly depending on the initial BHV-1 sero-prevalences. A field-infected animal has to be considered over its total lifespan as a latently infected carrier and as a potential source of infection. Therefore, BHV-1 diagnostics is crucial and often confronted with exceptional demands and requirements. For the sensitive and specific detection of BHV-1 antibodies, three different types of marker-independent tests are available: glycoprotein B (gB)blocking ELISAs, indirect ELISA tests, and the serum neutralisation test (NT). However, the gB-blocking assays represent the most IBR Workshop Angora, Beetal and Daira Din Panah) maintained at Rakh Khaire Wala Farm District Layyah. All the sera samples were first examined through Rose Bengal Plate Test (RBPT) and the samples found positive through RBPT were further confirmed through Serum Agglutination Test (SAT). Results: The overall prevalence of brucellosis was found to be 11% and 9.8% through Rose Bengal Plate Test and Serum Agglutination Test, respectively. Overall the females showed maximum prevalence of brucellosis in all breeds by RBPT (11.34%) and SAT (10.18%). Teddy goats showed the highest prevalence of brucellosis followed by Pak Angora, Beetal and Daira Din Panah. Discussion: This was a large scale screening of sera and in such cases the RBPT is an excellent tool and is the recommended method. This test has been widely employed in many successful Nationals Brucellosis Eradication Programmes. Based on the results, it can be concluded that the high prevalence of brucellosis is present in study area. Treatment of infected sheep and goats with antibiotics should be discouraged because the antibiotics may appear in the human food chain and this would be disastrous for the milk industry. Instead, efforts should be directed towards controlling and eradicating brucellosis from small ruminants by adopting integrated control strategies. Serological testing and slaughter of the animals that react positively with Brucella antigens successfully eradicated brucellosis in several countries. Vaccination against Br. melitensis may also be helpful in reducing the number of infected flocks and ultimately permit brucellosis control. Key words: goat, brucellosis, Pakistan sensitive test systems. In non-vaccinated animals, one ELISA system is commonly used as a screening test. In doubtful cases, a second ELISA principle can be employed as a confirmatory test. In contrast, BHV-1control according to the marker concept is based on vaccination with glycoprotein E (gE)-deleted marker vaccines. The marker-diagnostics is used to confirm the absence of gE-specific antibodies in "BHV-1 (gE-antibody) free" animals and to detect gE-antibodies in cattle infected with wild type virus. Therefore, determination of absence or presence of antibodies directed against gE is the only tool to discriminate marker-vaccinated from wild type virus infected animals. In addition, gE-blocking ELISAs are at the moment the only available test system for the detection of gE-specific antibodies. The possibilities and limitations of the diagnostic systems for BHV-1 will be presented and discussed, e.g. problems of the different sensitivity levels of conventional and gE-marker tests, the failure of testing milk samples for gE-antibodies as well as several phenomena like the “vaccinationphenomenon” and the “fresh sample-phenomenon”. It will be demonstrated that the choice of the appropriate test system and the establishment of an internal batch control are prerequisites of the optimal performance of BHV-1-ELISA systems. In addition, the reasons for the occurrence of so-called “pseudo-vaccinees” - animals that are not marker vaccinated but react clearly positive for BHV-1 and negative for gE - will be analysed. Finally, the lack of confirmatory marker tests must be compensated so far by evaluation of the epidemiological plausibility of the test results and by emphasising diagnosis on the herd level. Key words: IBR, BHV-1, diagnostics, marker tests 242 Prevalence and Risk Factors for Bovine Herpesvirus 1 Infection in Cattle Herds from Parana State, Brazil J. Dias, A. Alfieri, J. Ferreira Neto, E. Muller, A. Alfieri Universidade Estadual de Londrina, Departamento de Medicina Veterinária Preventiva, Londrina, Brazil The Brazilian cattle herd is constituted for approximately 205 million heads. The Paraná state, located in southern region of Brazil, is characterized by a developed livestock of beef and dairy cattle, with approximately 10.5 million heads. Throughout Brazil, the bovine herpesvirus (BoHV) infection is related to reproductive problems. Vulvovanigitis and meningoencephalitis are other clinical outcomes observed in infected herds. Serological surveys have showed a high Abortion – IBR Workshop 323
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130. évfolyam Különszám II. Vol
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The World Buiatrics Congress (WBC)
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lood and liver tissue. Absolute vit
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RNS causes increased lipid peroxida
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An experiment was conducted to stud
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2 Hungarian Institute of Agricultur
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and plasma HCO 3 - increased from H
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Materials and Methods: Serum sample
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vitamin A stores in liver, the hypo
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andomly assigned to one of five tre
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observed in group II of the animals
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The aim of the monitoring was to co
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health group (HG) categories for an
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lactating cows is sparse. Lotan and
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491 Effect of Acarbose on Milk Yiel
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499 Secondary Hepatogenous Photosen
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they all died in a two to three day
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and HCO 3 level. The concentration
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amount of saponins was observed in
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Water was offered ad-libitum. Feed
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Se, I and Co) more than 500 blood s
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541 Variability of Blood Profile an
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evaluated, highly significantly gre
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we measured sensitivity of the enti
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were 0.17; 0.13 °C (Max.: 0.73). D
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calving pregnancy period were the b
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2 University of Nottingham, Departm
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animals) was treated with 5 mg enro
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In dairying, good management practi
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Somatic cell count (SCC) is one of
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This investigation was run in some
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eproductive data showed that cows f
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(3.6%) and others (Bacillus spp., N
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602 Coagulase Gene Polymorphism of
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2 Institute of Veterinary Bacteriol
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an important part of the pathogens
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4 Universidade de Sao Paulo, Clinic
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incubated for one hour at 37 oC. Af
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In Belgium, an increasing amount of
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Conclusion: It can be concluded tha
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calves recovered after 4 to 10 days
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disease. Thus losses were estimated
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635 Prevalence of the Pulmonary Les
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Conclusions The sanitation program
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Italy. After the sample examination
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Results: The cows sera were collect
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(Mb) as co-infecting agent is becom
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distributed over 13 different fatte
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main infection sources to the urban
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686 Characterisation of Cattle Orig
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using EPFC. The intradermal tubercu
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three cattle farms each. Leptospiro
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(cELISA) only to definitively confi
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1 Islamic Azad University of Shahre
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Financial support: CNPq, CAPES, FIN
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731 Detection and Investigation on
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necropsied, tissue samples were tak
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aseline values. The mean HCO 3, PCO
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immunization with either the curren
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decrease in animal aggregation in p
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747 Effect of Dietary Supplementati
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755 Study of Pulmonary Adenomatosis
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1 University of Erciyes, Faculty of
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2006 was observed a small decreasin
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for O69 strain), and clinical signs
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2 University of Veterinary Medicine
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794 Serum Haptoglobin and Fibrinoge
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complete random design (CRD). The r
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- Ca, Mg, K, and Ca:P ratio were lo
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818 Cerebral Coenurosis in a Goat:
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concentrations of the goat milk, in
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was significant and showed that bre
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cm long and 1/48 cm diameter. Middl
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circular external skeletal fixator
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847 Llama Anesthesia for Orthopedic
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28 Preliminary Results of a General
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Israeli commercial dairy herds cons
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109 Association between Johne’s D
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195 Changes in Plasma Beta-Carotene
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In the last year 2006, in the perio
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serious economic concern in the fat
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was a cumulative incidence rate of
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The level of antibodies produced in
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1096 Determination of Risk Indicato
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ParaCheck, CSL/Biocor, Australia (C
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1111 The Prevalence of Hock Lesions
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Objectives: To evaluate two differe
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1126 Relationships between Paratube
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1134 A Comparison between Two Calf
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The present work aimed to investiga
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University College Dublin, School o
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214 Cystic Ovarian Diseases in Dair
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inseminator were tested in regressi
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University of Nottingham, Animal He
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tissue (8,6 cm 2 ) was twice as lar
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libido in unmotivated males. Simila
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groups according to their diameter
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improve embryonic development are m
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in each sample). On day 0, 104 cows
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1 Warsaw University of Life Science
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and durable spermatozoa. Within the
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motility and CM integrity (p=0.869
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the median number of attempted moun
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909 The effect of “GnRH” and
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3 COOPEVOLIA, Centre Zootechnique,
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923 Production of Bovine Blackleg V
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Institute of Physiology and Sanocre
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2 University College Dublin, Animal
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(I.M.) 1 mg of estradiol cipionate
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Lameness results in major economic
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methods; ANOVA (with binary classif
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Initially two radiographs were obta
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number of positive faecal samples d
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Pfizer Animal Health, Veterinary Me
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Key words: calf, active immunity, p
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1 University of Camerino, Veterinar
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vaccinated (Group II) with Pastobov
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# Antigens Association in diarrhea
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1015 Prevalence of Eimeria Infectio
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group showed higher serum lactate c
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thymus in calves and heifers with c
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concentrations of calves with umbil
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The goal of this paper is to expose
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187 Cooling Strategies to Improve M
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inspections, carried out by specifi
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The origin of Hungarian Grey cattle
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University of Warmia and Mazury in
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POSTER ABSTRACTS 1225 The Effect of
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multiple fracture (n=1, 1.3%). The
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position. A fusiform skin incision
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Conclusions: It is the first presen
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1 Faculty of Veterinary Medicine, A
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of 101.55±4.94 mmHg. Afterwards th
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In Mexico, there is a wide variety
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Cardiovascular disorders are common
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Conclusion: The total contamination
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Page 278 and 279: 390 Study on Attenuation Mechanisms
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Page 282 and 283: digestion. It works only on Apicomp
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Page 290 and 291: addition of mineral salt, during 12
Page 292 and 293: digestion could be improved with im
Page 294 and 295: Ltd., Blandford Forum, UK). The air
Page 296 and 297: cattle ocular squamous cell carcino
Page 298 and 299: pharmacokinetic/pharmacodynamic (PK
Page 300 and 301: Bovine Immunodeficiency Virus (BIV)
Page 302 and 303: significantly lower in groups vacci
Page 304 and 305: 5.00+0.67 and 4.94+0.15 mEq/l in th
Page 306 and 307: potable water and food, avoiding st
Page 308 and 309: equal distributions at 08:00 and 16
Page 310 and 311: electrochemical properties of the a
Page 312 and 313: Selenium was discovered as an essen
Page 314 and 315: growth by measuring BW at 15-day in
Page 316 and 317: influence of different risk factors
Page 318 and 319: performances of primiparous cows ar
Page 320 and 321: anaerobes. Aerobic bacteria isolate
Page 322 and 323: P4 12 to 24 hours before calving is
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Page 328 and 329: esults, supported by a decrease in
Page 330 and 331: international drivers of change. Th
Page 332 and 333: The performance of two groups of no
Page 334 and 335: spasm is accompanied by kyphosis, w
Page 336 and 337: occurrence of a crisis of stiffness
Page 338 and 339: Notices 336 XXV. Jubilee World Buia
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