Oral and Poster Abstracts

– Ca, Mg, K, and Ca:P ratio were lower in lactating goat .
The references ranges for serum biochemical analysis analysis can be
used for metabolic and nutritional disorders detection in goat
Key words: mineral metabolism, goat, lactating, Sahara
810 Metabolic Parameters of Dairy Goats Submitted to Artificial
Bioclimatic Conditions Similar to the Eastern Amazon Region
R. Pinho, J. Guimares, J. Ribeiro Filho, L. Martins, E. Castilho,
M. Borges, F. Santos, E. Da Silva
Universidade Federal de Viçosa, Veterinary Medicine, Viçosa, Brazil
This work deals with the adaptability of Alpine and Saanen female goat
breeds submitted to artificial bioclimatic conditions similar to those of the
the Eastern Amazon Region, when compared to animals raised under
normal typical bioclimatic conditions of regions where they demonstrate
seasonality. The study was conducted from April to July of 2007,
consisting of an adaptation period of 30 days and an experimental period
of 60 days. Group 1 (n=4) animals remained in the bioclimatic chamber
with temperature and air humidity control (8:00-12:00 hours: 30 ˚C;
12:00-18:00: 36 ˚C; 18:00-8:00: 26 ˚C; with 60% of average humidity;
and a 12 hour fotoperiod), thus simulating bioclimatic conditions of the
northern region of Brazil (next to Equator line), whereas group 2 (n=4)
was kept under influence of natural climatic variations of the season. The
physiological parameters of respiratory and cardiac frequencies, rectal
temperature and rumination behavior were measured twice a day, besides
blood collection twice a week for triiodotironine, tiroxine, cortisol, total
cholesterol, total proteins and albumine dosages. Water consumption
values were different, showing that the animals of group 1 consumed
more than the double (4386.3 mL/day) than those of group 2 (1663.2
mL/day). The ration consumption demonstrates there a difference both
between groups and periods (499.0 and 490.8 g/day, for groups 1 and 2,
respectively). There was no variation in the average values for corporal
weight and corporal score condition as a function of time, however,
during the experimental period, there was a difference in the weight of the
animals between groups (50.5 and 41.5 kg, for groups 1 and 2,
respectively). A difference was observed for the studied physiological
parameters between the morning and the afternoon, being the afternoon
values always higher than the ones in the morning. Cholesterol, albumine
and total protein concentrations demonstrated no difference between the
obtained average values during the observation period in function of the
days. No difference was registered between the obtained values during the
experimental period in Triiodotironine, tiroxine and cortisol
concentrations. The results indicated that female goats can be raised under
bioclimatic conditions, without modifying the related physiological
standards, making possible the production of embryos over the whole year
in regions where they demonstrate seasonality.
Key words: goats, metabolic, bioclimatology
811 CAE Virus Detection by PCR on Milk Samples
L. Gregory 1 , M. Lara 2 , M. Hasegawa 1 , E. Meira jr. 1 , H. Rizzo 1 ,
J. Rodrigues 3 , E. Durigon 3 , R. Castro 4
1 Universidade de Sao Paulo, Clinica Medica, Sao Paulo, Brazil
2 Instituto Biológico, Department of Clinical Science, Faculty of
Veterinary Medicine, Sao Paulo, Brazil
3 Universidade de Sao Paulo, ICB, Sao Paulo, Brazil
4 Universidade Federal de Pernambuco, Virology, Sao Paulo, Brazil
The caprine arthritis encephalitis is a multisystemic infectious disease
caused by a lentivirus and may affect animals at all ages and sex. The
major symptoms are leukoencephalomyelitis, cronical interstitial
pneumonia and hardening interstitial mastitis. CAE has world wide
distribution and in Sao Paulo, Brazil it is widely spread among the
herds. It was described that mammary glands from infected animals
had a gradual non inflammatory diffuse nodular hardening. It was
described decreased milk production, high electro-conductivity, high
levels of chlorides, high cell accounts, low protein levels, low fat levels
and low solid levels. The CAE virus detection on milk samples by the
PCR technique is characterized as a method with high specificity and
sensibility that provides fast results. The technique is able to identify
proteins or nucleic acids on samples with no living virus. In this study,
56 milk samples from infected animals were evaluated. 19 samples
were diagnosed positive by the PCR. The resultant cDNA were used to
the pol gene amplification. The positive control band was located on
the 286 pb position, which confirms the adequacy and sensibility using
PCR for the CAE virus detection on milk samples.
136 XXV. Jubilee World Buiatrics Congress 2008
Key words: CAE, caprine, ruminants, clinic, virology
812 Caprine Athritis Encephalitis Virus Detection by PCR on
Tissue Samples
L. Gregory 1 , M. Lara 2 , M. Hasegawa 1 , E. Meira jr. 1 , H. Rizzo 1 ,
J. Rodrigues 3 , E. Durigon 3 , R. Castro 4
1 Universidade de Sao Paulo, Clinica Medica, Sao Paulo, Brazil
2 Instituto Biológico, centro de sanidade animal, Sao Paulo, Brazil
3 Universidade de Sao Paulo, ICB, Sao Paulo, Brazil
4 Universidade Federal de Pernambuco, Virology, Recife, Brazil
In 1980 CAE was recognized as a viral disease caused by a lentivirus
from the Retroviridae family. In Brazil the caprine arthritis encephalitis
was introduced with irregular acquisition of imported animals.Two
distinguished clinical manifestations of the disease are characterized by
the major symptoms described: Leukoencephalomyelitis, that affects
kids and arthritis, most commonly observed on adult animals. Besides
these well defined clinical manifestations, it may be observed cronical
interstitial pneumonia and indurative interstitial mastitis. Some animals
may be diagnosed negatives when submitted to serological tests due to
the restricted gene expression, or in the early phases of the disease. On
these cases PCR tests present itself as a reliable diagnostic technique.
In this study, 33 tissue samples from brain, lungs and mammary glands
taken from 11 animals presenting arthritis or mastitis, were submitted
to PCR. The resultant cDNA was used to the pol gene amplification.
Two brain samples, one lung sample and one mammary gland sample
from two animals produced visible positive bands at the agarose gel.
The positive control band was located on the 286 pb position, which
confirms the adequacy and sensibility using PCR for the CAE virus
detection on tissue samples.
Key words: CAE, caprine, virology, clinic of ruminants
813 Mercury Poisoning in Goats
I. Maksymovych 1 , V. Vlizlo 2 , J. Nicpo_ 3 , M. Jankowski 3
1 Lviv national University of Veterinary medcine and Biotechnolgy,
Department of Clinical Diagnostics, Lviv, Ukraine
2 Institute of Animal Biology UAAS, Department of Clinical
Diagnostics, Lviv, Ukraine
3 Wroclaw University of Environmental and Life Sciences,
Department of Internal and Parasitic Diseases with Clinic for
Horses, Wroclaw, Poland
Pollution of the environment with heavy metals is registered near the
industrial centers. Cadmium, lead and mercury are the most toxically for
organism and do not cause any lesions of different organs and systems.
Materials and methods: To investigate the clinical symptoms,
biochemical indices of blood and urine, histological changes in kidneys
of goats at poisoning with mercury and to study the efficiency of
treatment measures was the purpose of our research work. 12 healthy
(control) and 6 mercury poisoned goats of local breed at the age from 9
months to 3 years were the materials for our investigation.
Results: Goats poisoning with mercury had caused the depression,
anorexia, fever, tachycardia, hypotonia of fore-stomach. The
concentration of urea to 9,7±1,99 mmol/l (P