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Oral and Poster Abstracts

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eproductive data showed that cows found positive to mycoplasma had<br />

lower reproductive efficiency. The percentage of cows with 3 or more AI<br />

per conception was higher (82%) for infected cows. The same pattern was<br />

observed on other parameters such as days-open <strong>and</strong> metritis incidence.<br />

The rate of pneumonic infections was highest after the onset of the<br />

outbreak. St<strong>and</strong>ard treatments were inefficient. Arthritis was also<br />

diagnosed in some cows showing enlarged joints <strong>and</strong> lameness. There<br />

was an increase of SCC between September <strong>and</strong> November in positive<br />

cows. During those 3 months 67%, 83% <strong>and</strong> 100% of the infected cows<br />

had SCC above 200,000 cells/ml with an average higher than 1,200,000<br />

cells/ml. The production level of the herd decreased from 26.5 kg in<br />

August to 22.6 kg of milk/day/cow in November. This report confirmed<br />

for the first time the association of M. bovis with clinical <strong>and</strong> subclinical<br />

mastitis in dairy cattle in Portugal. These results suggest that M. bovis<br />

should be considered in the differential diagnosis of mastitis in Portuguese<br />

dairy farms.<br />

Key words: mycoplasma bovis, dairy cattle, mastitis, arthritis,<br />

pneumonia<br />

587 Bulk Milk Tank Staphylococcus aureus UCF Count as<br />

Predictive Datum for Bovine Herd’s Infection Level<br />

G. Varisco 1 , L. Bertocchi 1 , N. Contessi 2 , M. Tranquillo 1 , G. Bolzoni 1<br />

1 Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia<br />

Romagna Brescia, Brescia, Italy<br />

2 Centro Miglioramento Latte, Brescia, Italy<br />

Aim of this work is to verify the predictivity of bulk milk tank<br />

(BMT) analysis as simple tool for epidemiological surveillance system on<br />

herd’s Staphylococcus aureus infection level. The comparison between<br />

BMT analysis results <strong>and</strong> single cow milk (SCM) analysis results was<br />

investigated to verify the relationship between BMT Staph. aureus UFC<br />

value <strong>and</strong> dairy herds infection rate. During 2005, 47.208 cow milk<br />

samples were analysed (7.166 positive, 38.530 negative <strong>and</strong> 1.512 to<br />

much polluted)) of 31 dairy herds (approximately 4.000 cows were<br />

checked). Staph. aureus count in BMT (UFC) was found to<br />

decrease during time, due to dairy Staph. aureus infection rate reduction<br />

at cow level. Statistical analysis shows that within herds the prevalence is<br />

1.28 grater (exp 0.25) every one UFC log unit (p<br />

7 log) were removed by the bedding treatment.<br />

Conclusions: The results indicate that applying DryMaxx bedding<br />

treatment to the bacterial suspension facilitated the removal of the bacterial<br />

suspension from the cow mat. We suggest applying DeLaval bedding<br />

treatment to DeLaval cow mats, letting the bedding treatment remain on<br />

the cow mat for at least 30 minutes, <strong>and</strong> then sweeping off the bedding<br />

treatment <strong>and</strong> removing it from potential future contact with the cow.<br />

589 Evaluating the Teat Condition Performances of a New Teat<br />

Spray in an Automatic Milking Environment<br />

X. Goossens 1 , L. Bommelé 2 , W. Ingalls 3 , T. Hemling 3<br />

1<br />

DeLaval, PP Milk Quality & Animal Health, Gent, Belgium<br />

2<br />

Ghent University, Biocenter Agri-Vet, Melle, Belgium<br />

3<br />

DeLaval, PP Milk Quality & Animal Health, Kansas City, United<br />

States<br />

Automatic milking is a growing practice in dairy farming. In an automatic<br />

milking system the cows are milked more frequently <strong>and</strong> the variation in<br />

the interval between milkings is greater than with conventional milking.<br />

As the automatic milking creates a new <strong>and</strong> different management <strong>and</strong><br />

environment with different challenges for the teats, new teat dips with the<br />

objective to maintain good teat condition when sprayed in an automatic<br />

milking system should be tested in the system itself. Results abstracted<br />

from conventional milking parlours cannot be the only basis to evaluate<br />

teat dips in this new way of milking. Teat condition studies done in<br />

conventional milking parlours, use a split udder or split herd design. In<br />

the conventional milking parlours this dipping or spraying is done by<br />

human interaction <strong>and</strong> can be done selectively with the right product on<br />

the correct teats. In automatic milking systems, teat spraying is done<br />

automatically. Technically it is not possible to spray the teats of<br />

different cows with different products. And because of the spraying it<br />

is not possible to spray only two teats of each cow without hitting the<br />

two control teats. This makes it impossible to do a side by side control<br />

trial in a robotic system. The objective of this study was to evaluate a<br />

new teat spray for an automatic milking system in a commercial dairy<br />

farm environment with a positive control. To evaluate teat condition<br />

performance of a new peroxide teat spray (DeLaval Prima) in an<br />

automatic milking system we made use of a switch back design<br />

(Rasmussen et al. 2001). Control product was Delaval Proactive Plus, a<br />

teat dip with well know teat condition performance. Before the start of<br />

the trial, the teats had already been sprayed for a long period with the<br />

control product. During the trial period of 18 weeks, the experimental<br />

product <strong>and</strong> the control product were switched every 6 weeks. At the start<br />

<strong>and</strong> every two weeks during the trial period, teat skin condition, teat end<br />

condition <strong>and</strong> orifice thickness were scored by the same trained person<br />

using an ordinal scale. Every two weeks individual SCCs were recorded.<br />

Weather data were gathered throughout the trial. The switch back design<br />

should help to evaluate the teat condition performances of a new teat<br />

spray in an automatic milking environment with one robot.<br />

590 Prevalence of Intramammary Infection in Pregnant Heifers<br />

M. Vasil, J. Bires<br />

University of Veterinary Medicine, Animal Husb<strong>and</strong>ry <strong>and</strong> Veterinary<br />

Science, Kosice, Slovakia<br />

Examination carried out in 3 farms on 110 heifers. Clinical<br />

examination of the udder <strong>and</strong> bacteriological tests of quarter secretion

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