Oral and Poster Abstracts

767 Effect of Zinc from Zinc Sulfate on Ewes’ Weight, Milk Yield,
Zn Concentrations in Serum and Serum Alkaline Phosphates
Activity of Varamini Ewes
Ab. Zali, Ha. Amanlou, Al. Nik khah, Ah. Ahmad, Ka. Rezayazdi,
Ma. Ganjkhanlou
Animal Sciences Group, Animal Science, Karaj, Iran
This experiment was conducted to investigate the effect of feeding
supplemental zinc (zinc sulfate) in different levels (0, 15 and 30 mg/kg)
on ewes weight, milk production, Zn concentrations in serum and
serum alkaline phosphates activity. Thirty lactating Varaminni ewes
were assigned to three experimental groups according to their live body
weights, milk production and lambs sex in a completely randomized
design. Ewes were fed a basal diet containing alfalfa, wheat straw,
cottonseed meal, barley grain, wheat bran, cracked corn and vitaminmineral
supplements at 3.2% of BW to meet NRC requirements for
protein, energy, macro minerals and micro minerals. The basal diet
contained 15 mg/kg Zn and Zinc sulfate was added to the basal diet to
supply 30 or 45 mg/kg of dietary zinc. Milk yielded, milk composition
and ewes’ weight was recorded at 7 and 21 days intervals respectively.
Samples of the blood were taken three times (0, 35 and 70) for
determination of Concentration of Zn, Cu and Fe, Na, K, Ca in serum.
Also serum alkaline phosphates concentration of ewes was measured.
Milk yield, milk composition and ewes’ weight of ewes were not
affected by supplemental zinc (p>0.05). Alkaline phosphatase
concentration was increased with supplemental zinc linearly and this
increase was significant (p0.05).
Key words: supplemental zinc, zinc sulfate, varaminni ewes
768 Microscopic Comparison among Different Treatments Effects
for Second Intention Skin Healing in Sheeps
R. Godoy, JE. Barroso, FH. Ximenes, M. Castro, V. Mustafa, C.
Leite, C. Pereira, L. Gouvea, L. Gontijo, JR. Borges
Universidade de Brasília, FAV, Brasília-DF Brazil, Brazil
Objective: Compare the effect of Stryphnodendron adstringens
solution, Dakin’s solution, povidone-iodine and water for second
intention skin healing in sheeps.
Materials and methods: Four cutaneous wounds were made on both sides
of thorax in 6 sheeps, with a circular “punch” of 0,8 cm of diameter,
similar to the procedure for obtaining biopsies. Treatment began after 15
hours and was maintained once daily. It was used sequentially, the
treatments: T1. Water; T2. Stryphnodendron adstringens solution at
10%, T3. Dakin’s solution (NaClO 0.5%) and T4. Povidone-iodine
(PVPI) 0.1%. The biopsies, performed in the right and left sides, on days
7 (D7) and 14 (D14), respectively, after the production of wounds, were
sent for histopathologic analysis that evaluated swelling, exudate of
polymorphonuclear (PMN), vascularization, mononuclear infiltrate,
fibrosis, ulcer and reepitelization. The measurement standard of the
factors cited were: Degree 0 = Absence; Degree 1 = Mild, Degree 2 =
Moderate, Degree 3 = Intense.
Results: The biopsies performed in D7 showed that the wounds treated
with Dakin’s solution and Barbatiman’s solution showed degree 1 of
swelling, the water treated, degree 2, followed by mild to moderate in
the treatment with PVPI. Regarding fibrosis, T1 and T2 showed degree
1 and in the others treatments mild to moderate degree were observed.
The others parameters evaluated did not differ between treatments,
with degree 3 to PMN exudate and ulcer, degree 1 to vascularization
and absence to mononuclear infiltrate. In D14, the degree of swelling in
the T1 and T3 was 0, in the T4 ranged from absence to mild and
resulted in degree 1 in T2. Regarding PMN exudate, T3 resulted in
degrre 0, in T1 and T4 was predominantly degree 1 and in T2 degree 3
were measured. The vascularization in T2 showed degree 1 and in the
others treatments were observed degree 2. The mononuclear infiltration
was evalueted as degree 2 in T1 and as degree 1 in the others
treatments. In T2, the degree of fibrosis observed was 1, in the T1 and
T4 it were similar, presenting degree 2, and T3 ranged from moderate
to intense. With regard to ulcer it obtained degree 0 in T3 and T4,
degree 1 in T1 and degree 2 in T2. For reepitelization, T3 and T4
resulted in degree 3, followed by moderate to intense degree to T1 and
T2 with degree 1. Conclusion: Second intention skin healing in sheeps
was more efficient in water and Dakin’s solution treatments.
Key words: Stryphnodendron adstringens, histopathology, ruminant,
wound
769 Echodopplercardiographic Evaluation in Santa Ines Sheeps
JE. Barroso, M. Mendes, CE. Silva, R. Godoy, JR. Borges
Universidade de Brasília, FAV, Brasília-DF Brazil, Brazil
Objective: Evaluate the cardiac size and function in Santa Ines adult
sheeps to establish physiologic reference values.
Materials and Methods: 30 sheeps not pregnant, aged between one
and five years and average weight of 34.8 ± 7.41 kg were used. In right
lateral decubitus and with a 2-4 MHz bandwidth microconvex
transducer, in M-Mode, it was measured in cm: in diastole, the
thickness of right ventricle wall (RVWd), of interventricular septum
(VSd), of left ventricular wall (LVWd), diameter of right ventricle
(RVd) and of left ventricle (LVd), and in systole, thickness of the
interventricular septum (VSs), of left ventricle wall (LVWs) and left
ventricle diameter (LVs). Diameters of systolic left atrium (LA) and
diastolic aorta (AO) were measured in cm and the left atrium/aorta ratio
(LA/AO) was established. Shortening fraction (SF%), was calculated
with the formula (LVd-LVs/LVd)x100. Pulmonary (VPul) and aortic
(VAO) velocity flow were measured in m/s and pulmonary (PGPul)
and aortic (PGAO) pressure gradients in mmHg. It was evaluated
pulmonary and aortic regurgitation by color flow mapping. Right
parasternal long-axis left ventricular outflow view was used to obtain
RVWd, RVd, LVd, LVs, LVWs, LVWd, VSs and VSd values. The
same view was used on the heart base to get LA and AO values. VPul
and VAO were obtained, with pulsed Doppler, in the right parasternal
oblique left ventricular right ventricular outflow view and the left
parasternal apical view, respectively.
Results: The average values found for RVd and LVd were 1.34±0.46
and 3.28±0.38, respectively, maintaining a 1 to 3 approximately ratio.
RVWd (0.39±0.09) was half of LVWd value (0.62±0.12) and the
LVWs, LVs, VSd and VSs average dimensions were 1.06±0.2,
2,19±0.29, 0.92±0.12 and 1.18±0.15 respectively. FS found was
32.96±6.19. The LA diameter (2.78±0.29) was similar to AO
(2.44±0.27), and LA/AO ratio was equal to 1.15. VAO (0.85±0.12) and
VPul (0.85±0.1) values were similar, as well as and PGPul (2.95±0.71)
and PGAO (2.93±0,82). Discreet aortic and pulmonary regurgitation
with low velocity and short duration were observed in 13% and 50% of
the examined animals, respectively.
Conclusion: This study establishes echodopplercardiographycs
reference values to Santa Ines sheeps. Others parameters, as well as
different breeds, should be evaluated.
Key words: ovine, cardiology, cardiac size, cardiac function
770 Aspects Regarding Maedi Visna Diseases Incidence and
Prevalence in Covasna County
R. Popescu1 , C. Horhogea2 , B. Siko1 1 Regional Veterinary Laboratory, Virology, Sfintu Gheorghe,
Romania
2 University of Agricol Sciences and Veterinary Medicine, Veterinary
Medicine, Iasi, Romania
Objectives of study: The researches were made to establish the
incidence and prevalence of Maedi Visna infection in Covasna County.
Materials and Methods: The researches were made on 8300 samples
from Covasna County sheeps, with different owners during 2003-2007.
For the serological exams the folowing ELISA kits were used:
CHEKIT CAEV/MVV Screening, IDEXX Lab., Caprine Arthritis-
Encephalitis Virus Antibody Test Kit, VMRD Inc., ELISA MAEDI-
VISNA/CAEV Serum, Pourquier Institut, Serological Diagnosis of
Maedi-Visna By testing for Gp135 antibodies in serum using the agargel
immunodiffusion method, Pourquire Insitut.
Results: In 2003 were tested 2019 serum samples through ELISA, 405
being positive. In 2004 were tested 830 samples revealing the fact that
193 were positive, representing a prevalence of 23%.
In 2005 were tested 1545 samples, 390 being positive, and revealing a
prevalence of 25%. In 2006 were tested 1140 serum samples,843 being
negative and 297 positive showing a prevalence of 25%.The tests
revealed the fact that in 2006 the diseases was extended in all 38
circumscriptions of the Covasna County. In 2007 the serological tests
executed on 1582 samples showed that 1107 samples were negative
and 475 were positive, indicating a prevalence of 30%.
Conclusions: The researches made between 2003-2007 showed
variations of the Maedi Visna infections incidence and prevalence. It
was observed that the diseases incidence in 2004 was of 84 positive
cases, in 2005 was revealed an increasing till 194 positive cases, in
Sheep, Goat and Buffaloes 125