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(cELISA) only to definitively confirm results obtained in the aforementioned test. It performed a trial study to assess the status of brucellosis in the state of Lara, Venezuela. Initially, milk ring tests were performed in 320 farms, with 30% of them testing positive. Serum samples were collected from all animals of farms that tested positive to ring test. A total of 13,351 serum samples were collected and tested with RBT and cELISA assays. Positive index observed with RBT was lower when compared to cELISA (0.51 % vs 6.36 %, respectively). Furthermore, both tests confirm that positive index in the state of Lara is very low. However, relative sensitivity observed in RBT is also very low when compared to cELISA. This apparent low sensitivity could result in a high number of false negative animals that, consequently, will not be eliminated from the herds. Thus, this would constitute a constant source of infection to other animals and people. Key words: brucellosis, Rose Bengal test, competitive ELISA 708 In vitro Evaluation of Different Antibiotic Combinations used for Treatment of Pasteurellaceae from Bovine Respiratory Disease (BRD) J. Ganiere 1 , L. Denuault 1 , O. Bidaud 2 , J. Swinkels 3 , F. Payot 2 1 Nantes Veterinary School, Research Unit of Infectious Diseases, Nantes, France 2 Intervet Schering-Plough, Research Unit of Infectious Diseases, Angers, France 3 Intervet Schering-Plough, Int. Marketing, Boxmeer, The Netherlands Objectives: In the field, cattle with BRD may receive different antibiotics simultaneously or successively. The objective of this study was to describe the interaction of cefquinome with other antibiotics frequently used to treat BRD. Materials and Methods: The effect of combinations of cefquinome with oxytetracyclin, marbofloxacin, florfenicol, tilmicosin and tulathromycin against the Pasteurellaceae involved in BRD: M. haemolytica, P. multocida and H. somni was tested in vitro. One strain of each bacterium isolated from clinical cases was used to assess the interaction of the combinations, determined by the Fractional Inhibitory Concentration Index (FICI) and confirmed by time-kill kinetics. Four interactions were concerned: FICI
lesions in other organs. Young animals most frequently become infected by ingestion of infected milk. We present a case report of tuberculous meningoencephalitis in a dairy calf that was less than one year old, who presented nervous signs (recumbency, irritability and aggression, weakness, dizziness), which progressed to death in three days. At necropsy, white nodules were found in the liver, lungs and diaphragm. The clinically suspected diagnosis was rabies, but results were negative for direct immunofluorescence and intracerebral inoculation in mice. Brain samples and spinal cord were preserved in 10% formalin for histopathological analysis (HE staining). There was severe granulomatous meningomyelitis with Langhans’ giant cells. In cerebral neuropile there were some granulomas with a central area of necrosis, perivascular cuffing with mononuclear cells, gliosis and hyperemia. The lung had many caseated granulomas, a typical finding in bovine tuberculosis, with central necrosis, surrounded by epithelioid macrophages, lymphocytes, plasma cells, Langhans’ giant cells and fibrosis at the periphery. There was mild calcification of the pulmonary granulomas. Samples of lung and brain were decontaminated by the Petroff method and inoculated into Petragnani and Stonebrink media and M. bovis was isolated. This case report shows that bovine tuberculosis should be included in the differential diagnosis of bovine neurological disorders. According to Brazilian regulation, sanitary measures in the heard infected (test-andslaughter) should be adopted. FAPESP Key words: tuberculosis, Mycobacterium bovis, meningoencephalitis, calf 712 A Study on Diagnosis, Pathogenesis of Pseudomonas aeruginosa Isolated from Different Cases of Human and Animals in Mosul City - Iraq D. Ali 1 , B. Ismaeel 2 1 College of Veterinary Medicine, University of Mosul, Iraq, Department of Microbiology, Mosul, Iraq 2 College of Veterinary Medicine, University of Dohuk, Department of Microbiology, Duhok, Iraq The current study tackles the isolation and identification of Pseudomonas aeruginosa from different human infection cases (wounds, otitis media, urine, stool, and burns) in Al-Zahrawi, Al-Salam and Ibn-Al-Atheer hospitals at Mosul city. From animals (sheep, cows, goats) samples were also collected (wounds, otitis, urine, feces and mastitis) cases for P. aeruginosa isolation and identification. The morphological, cultural and biochemical tests confirmed 43 (17.4%) isolates as P. aeruginosa from 250 human samples, and 17 (10.3%) from 165 animal samples. P. aeruginosa human and animals’ isolates virulence factors represented by enzymes (protease, lipease, lecithinase, b-lactamase gelatinase, coagulase, haemolysin, toxins) and capsule production were studied; 80% of human isolates showed capsule presence, while all animal isolates (100%) contained capsule. The pigments production especially pyocyanin, which differentiates P. aeruginosa from other microorganisms, was detected in 80% of the human isolates and in 100% of the animal isolates. The study showed similarity between P. aeruginosa of human and animal isolates in their virulence factors production ability. The study results revealed that 15 pathogenic isolates from 43 tested human isolates and 10 pathogenic isolates from 17 tested animal isolates produced positive results for an exotoxin A production in rabbit skin reaction test, through inducing hypersensitivity reactions of type I (early or immediate) and of delayed type (Type IV). Baby suckling mice test and rabbit ileal loop test confirmed that both human and animal P. aeruginosa isolates produced an enterotoxin. The histopathological study revealed different changes represented by elongation and blunted villi with necrosis. 713 Bovine Paralityc Rabies: Prevention and Control in Mexico M. Manzano 1,2 , S. Vázquez-Morón 2 , R. Ramirez 1 , M. Orozco 3 , J. Echevarría 2 , A. De Vega 1 1 Comite de Fomento y Salud Animal, Campana de Rabia, Puebla, México, Mexico 2 Instituto de Salud Carlos III, Servicio de Microbiologia Diagnostica, Madrid, Spain 3 Unión Ganadera Regional Sierra Oriente, Gerencia, Teziutlan, Mexico Bovine paralytic rabies is a contagious and fatal disease that affects a large number of productive animal species and also humans, creating a risk both to human and animal health. In America this involves a serious problem. One of the determining factors to prevent cases of rabies in animals and in humans is, without any doubt, the diffusion and training on measures of prevention and control of the disease. A DVD on this kind of measures was recorded in the City of Puebla, Mexico, by the Committee of Promotion and Animal Health and the Foundation “PRODUCE”, Puebla. First, the DVD has been presented all over the country, and, on a subsequent occasion, it was presented in the RITA (The international meeting on research advances in rabies control in the Americas) meetings of Brazil and Mexico, in coordination with the Panamerican Health Organization. The DVD was distributed to all American countries through these meetings.The mentioned DVD shows two aspects of this problem: in the first part a case of an outbreak of paralytic rabies is narrated in the colloquial language of the cattle farmers from the northern mountains belonging to the Puebla State; while the second part includes recommendations about the cold chain, antirabies vaccination, sending specimens to the laboratory, classification of bats and control of hematophagous bats.Furthermore, molecular studies are currently being realized in the Instituto de Salud Carlos III (Madrid, Spain) to complement the field work made in Mexico. For all the reasons exposed it is of great interest to share this experience with European Buiatric groups. 714 Effect of Cow Vaccination against BRSV and PI3 on Immune Status ante Partum and the Transfer of Colostral Antibodies to Calves B. Makoschey 1 , R. Brunner 2 , M. Koenig 3 , C. Gumpert 4 , H. Heckert 4 1 Intervet Schering-Plough, Int. Marketing, Boxmeer, The Netherlands 2 Intervet Schering-Plough, Businessunit Cattle, Unterschleissheim, Germany 3 Justus-Liebig-Universität Gießen, Faculty of Veterinary Medicine, Institute of Virology, Giessen, Germany 4 Freie Universität Berlin, Clinic for Ruminants and Pigs, Berlin, Germany Objectives: The effect of immunization of pregnant cows with respiratory pathogens (Bovine Respiratory Syncytial Virus (BRSV) and Parainfluenza Type 3 (PI3)) on the antibody levels in the serum and colostrum as well the transfer of the colostral antibodies to the newborn calves should be determined. Material and Methods: The study was performed on a dairy farm where the dams and their calves are housed at two different locations. A commercial vaccine containing inactivated antigens of BRSV, PI3 and Mannheimia haemolytica (M. haemolytica) (Bovipast ® RSP / Bovigrip RSP plus, Intervet) was used. Twenty-six dams (n=26) were vaccinated twice (6-8 and 2-4 weeks before the anticipated date of calving). A group of 19 cows was not vaccinated with the respiratory vaccine. In addition, all cows (n=45) were routinely immunized with a neonatal calf diarrhea containing inactivated bovine rotavirus, bovine coronavirus and E. coli at 6 weeks before calving. Colostrum was collected from each cow and fed to the respective calf at a volume of at least 4 liter within the first 12 hours of life. Blood samples of the dams were taken at the time of the first vaccination and around calving. Moreover, samples of the first milkings were collected. Blood samples of the calves were taken two days after birth. Neutralising antibody titers against BRSV and PI3 were measured in the serum and colostrum samples. Results: The interpretation of results regarding BRSV was troubled by high pre-vaccination antibody levels in a certain proportion of animals. Significantly higher antibody titres against BRSV were found in dams from the vaccinate group after adjustment of measured titres by subtracting individual pre-vaccination values. Moreover, there was a correlation between the BRSV antibody titer in the cows and the titers measured in the calves. With regard to PI3, vaccination of dams resulted in a booster of neutralizing antibodies and a transfer of the antibodies to their newborn calves via colostrum regardless the pre-vaccination titer against PI3. Moreover, the PI3 antibody titers in the cows correlated well with the titers measured in the two days old newborns. Conclusions: Cow vaccination against BRSV and PI3 could be a strategy to achieve more homogeneous antibody levels in the serum of the dams and their calves and can therefore complement the active immunisation program in the calves. 715 Detection of Prevalence of Rotaviral and Corona Viral Diarrhea in Newborn Calves B. Shojaee 1 , F. Hemmatzadeh 1 , H. Momtaz 1 , M. Ahmadi bozorg 1 , B. Khoramian 2 , M. Eslampour 2 Infectious and Zoonotic Deseases (Public Health) 105
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130. évfolyam Különszám II. Vol
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The World Buiatrics Congress (WBC)
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lood and liver tissue. Absolute vit
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RNS causes increased lipid peroxida
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An experiment was conducted to stud
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2 Hungarian Institute of Agricultur
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and plasma HCO 3 - increased from H
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Materials and Methods: Serum sample
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vitamin A stores in liver, the hypo
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andomly assigned to one of five tre
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observed in group II of the animals
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The aim of the monitoring was to co
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health group (HG) categories for an
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lactating cows is sparse. Lotan and
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491 Effect of Acarbose on Milk Yiel
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499 Secondary Hepatogenous Photosen
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they all died in a two to three day
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and HCO 3 level. The concentration
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amount of saponins was observed in
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Water was offered ad-libitum. Feed
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Se, I and Co) more than 500 blood s
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541 Variability of Blood Profile an
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evaluated, highly significantly gre
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we measured sensitivity of the enti
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were 0.17; 0.13 °C (Max.: 0.73). D
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calving pregnancy period were the b
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2 University of Nottingham, Departm
Page 56 and 57: animals) was treated with 5 mg enro
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Page 60 and 61: Somatic cell count (SCC) is one of
Page 62 and 63: This investigation was run in some
Page 64 and 65: eproductive data showed that cows f
Page 66 and 67: (3.6%) and others (Bacillus spp., N
Page 68 and 69: 602 Coagulase Gene Polymorphism of
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Page 76 and 77: incubated for one hour at 37 oC. Af
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Page 80 and 81: Conclusion: It can be concluded tha
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Page 84 and 85: disease. Thus losses were estimated
Page 86 and 87: 635 Prevalence of the Pulmonary Les
Page 88 and 89: Conclusions The sanitation program
Page 90 and 91: Italy. After the sample examination
Page 92 and 93: Results: The cows sera were collect
Page 94 and 95: (Mb) as co-infecting agent is becom
Page 96 and 97: distributed over 13 different fatte
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Page 100 and 101: 686 Characterisation of Cattle Orig
Page 102 and 103: using EPFC. The intradermal tubercu
Page 104 and 105: three cattle farms each. Leptospiro
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Page 110 and 111: Financial support: CNPq, CAPES, FIN
Page 112 and 113: 731 Detection and Investigation on
Page 114 and 115: necropsied, tissue samples were tak
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Page 120 and 121: decrease in animal aggregation in p
Page 122 and 123: 747 Effect of Dietary Supplementati
Page 124 and 125: 755 Study of Pulmonary Adenomatosis
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Page 128 and 129: 2006 was observed a small decreasin
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Page 132 and 133: 2 University of Veterinary Medicine
Page 134 and 135: 794 Serum Haptoglobin and Fibrinoge
Page 136 and 137: complete random design (CRD). The r
Page 138 and 139: - Ca, Mg, K, and Ca:P ratio were lo
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Page 142 and 143: concentrations of the goat milk, in
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Page 146 and 147: cm long and 1/48 cm diameter. Middl
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Page 150 and 151: 847 Llama Anesthesia for Orthopedic
Page 152 and 153: 28 Preliminary Results of a General
Page 154 and 155: Israeli commercial dairy herds cons
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109 Association between Johne’s D
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195 Changes in Plasma Beta-Carotene
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In the last year 2006, in the perio
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serious economic concern in the fat
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was a cumulative incidence rate of
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The level of antibodies produced in
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1096 Determination of Risk Indicato
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ParaCheck, CSL/Biocor, Australia (C
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1111 The Prevalence of Hock Lesions
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Objectives: To evaluate two differe
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1126 Relationships between Paratube
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1134 A Comparison between Two Calf
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The present work aimed to investiga
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University College Dublin, School o
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214 Cystic Ovarian Diseases in Dair
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inseminator were tested in regressi
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University of Nottingham, Animal He
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tissue (8,6 cm 2 ) was twice as lar
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libido in unmotivated males. Simila
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groups according to their diameter
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improve embryonic development are m
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in each sample). On day 0, 104 cows
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1 Warsaw University of Life Science
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and durable spermatozoa. Within the
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motility and CM integrity (p=0.869
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the median number of attempted moun
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909 The effect of “GnRH” and
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3 COOPEVOLIA, Centre Zootechnique,
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923 Production of Bovine Blackleg V
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Institute of Physiology and Sanocre
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2 University College Dublin, Animal
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(I.M.) 1 mg of estradiol cipionate
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Lameness results in major economic
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methods; ANOVA (with binary classif
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Initially two radiographs were obta
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number of positive faecal samples d
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Pfizer Animal Health, Veterinary Me
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Key words: calf, active immunity, p
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1 University of Camerino, Veterinar
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vaccinated (Group II) with Pastobov
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# Antigens Association in diarrhea
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1015 Prevalence of Eimeria Infectio
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group showed higher serum lactate c
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thymus in calves and heifers with c
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concentrations of calves with umbil
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The goal of this paper is to expose
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187 Cooling Strategies to Improve M
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inspections, carried out by specifi
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The origin of Hungarian Grey cattle
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University of Warmia and Mazury in
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POSTER ABSTRACTS 1225 The Effect of
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multiple fracture (n=1, 1.3%). The
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position. A fusiform skin incision
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Conclusions: It is the first presen
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1 Faculty of Veterinary Medicine, A
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of 101.55±4.94 mmHg. Afterwards th
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In Mexico, there is a wide variety
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Cardiovascular disorders are common
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Conclusion: The total contamination
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Material and methods: The trial inc
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detection of M. wenyonii infection.
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390 Study on Attenuation Mechanisms
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POSTER ABSTRACTS 1152 Detection of
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digestion. It works only on Apicomp
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determined. In addition the surface
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Africa and Europe, which causes mor
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Results: On D0, the animals include
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addition of mineral salt, during 12
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digestion could be improved with im
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Ltd., Blandford Forum, UK). The air
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cattle ocular squamous cell carcino
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pharmacokinetic/pharmacodynamic (PK
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Bovine Immunodeficiency Virus (BIV)
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significantly lower in groups vacci
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5.00+0.67 and 4.94+0.15 mEq/l in th
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potable water and food, avoiding st
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equal distributions at 08:00 and 16
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electrochemical properties of the a
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Selenium was discovered as an essen
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growth by measuring BW at 15-day in
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influence of different risk factors
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performances of primiparous cows ar
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anaerobes. Aerobic bacteria isolate
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P4 12 to 24 hours before calving is
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exists about Mb prevalence in dairy
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frequency of animals with BoHV anti
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esults, supported by a decrease in
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international drivers of change. Th
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The performance of two groups of no
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spasm is accompanied by kyphosis, w
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occurrence of a crisis of stiffness
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Notices 336 XXV. Jubilee World Buia
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